Issue 98, 2016
From the journal:

Chemical Communications

Ratiometric and photoconvertible fluorescent protein-based voltage indicator prototypes†

A. S. Abdelfattah,a   V. Rancic,b   B. Rawal,b   K. Ballanyib  and  R. E. Campbell*a  

* Corresponding authors

a Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada
E-mail: robert.e.campbell@ualberta.ca

b Department of Physiology, University of Alberta, Edmonton, Alberta, Canada

Abstract

To expand the toolbox of fluorescent protein-based voltage indicators, we explored two distinct protein design strategies. Using these design strategies, we created three new voltage indicators: a red intensiometric voltage indicator (tdFlicR Δ110AR), a green/red ratiometric voltage indicator (tdFlicR-VK-ASAP), and a green to red photoconvertible voltage indicator (FlicGR1).

Graphical abstract: Ratiometric and photoconvertible fluorescent protein-based voltage indicator prototypes

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Article information

DOI
https://doi.org/10.1039/C6CC06810C
Article type
Communication
Submitted
19 8 2016
Accepted
14 11 2016
First published
14 11 2016
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2016,52, 14153-14156

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Ratiometric and photoconvertible fluorescent protein-based voltage indicator prototypes

A. S. Abdelfattah, V. Rancic, B. Rawal, K. Ballanyi and R. E. Campbell, Chem. Commun., 2016, 52, 14153 DOI: 10.1039/C6CC06810C

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