Issue 32, 2015

An enzyme-activatable probe with a self-immolative linker for rapid and sensitive alkaline phosphatase detection and cell imaging through a cascade reaction

Abstract

We report the design and synthesis of a novel probe (1) for ALP assay by incorporating a self-immolative linker between a phosphate moiety and resorufin. Because of its good biocompatibility and rapid cell internalization, this probe also exhibited great potential for real-time monitoring of endogenous phosphatase activity in living cells.

Graphical abstract: An enzyme-activatable probe with a self-immolative linker for rapid and sensitive alkaline phosphatase detection and cell imaging through a cascade reaction

Supplementary files

Article information

Article type
Communication
Submitted
03 2 2015
Accepted
18 3 2015
First published
18 3 2015

Chem. Commun., 2015,51, 7031-7034

An enzyme-activatable probe with a self-immolative linker for rapid and sensitive alkaline phosphatase detection and cell imaging through a cascade reaction

H. Zhang, C. Xu, J. Liu, X. Li, L. Guo and X. Li, Chem. Commun., 2015, 51, 7031 DOI: 10.1039/C5CC01005E

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