Issue 40, 2014

Tracking chemical reactions on the surface of filamentous phage using mass spectrometry

Abstract

Chemical modification of phage libraries has allowed the in vitro evolution of ligands having properties not provided by natural polypeptides. The development of novel and more diverse chemical reactions on phage was hampered by the lack of analytical methods to efficiently monitor the reaction products on the more than 10 000 kDa large filamentous phage particles. Herein, we present a strategy to detect chemically modified peptides on phage based on enzymatic release of peptide from phage and mass spectrometry analysis.

Graphical abstract: Tracking chemical reactions on the surface of filamentous phage using mass spectrometry

Supplementary files

Article information

Article type
Communication
Submitted
30 9 2013
Accepted
14 11 2013
First published
14 11 2013

Chem. Commun., 2014,50, 5267-5269

Tracking chemical reactions on the surface of filamentous phage using mass spectrometry

S. Chen, J. Touati and C. Heinis, Chem. Commun., 2014, 50, 5267 DOI: 10.1039/C3CC47496H

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