Issue 36, 2013
From the journal:

Chemical Communications

Quantitative detection of single base mutation by combining PNA hybridization and MALDI-TOF mass analysis†

Youn Jee Yeo,‡a   Kyoungmin Roh,‡b   Joo Young Bang,a   Eun Hee Lee,a   Hyung Soon Park*a  and  Dong-Eun Kim*b  

* Corresponding authors

a R&D Center, Diatech Korea Co. Ltd., Seoul 138-200, Republic of Korea

b Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea
E-mail: kimde@konkuk.ac.kr, hspark@diatech.co.kr
Fax: +82-2-3436-6062
Tel: +82-2-2049-6062

Abstract

Peptide nucleic acid (PNA) probes were designed to bind to the internal reference sequence and the single base mutation sequence within PCR-amplified DNA templates. PNAs hybridized to the target sequences on DNA were analyzed using MALDI-TOF mass spectrometry. Accurate quantification of the relative amount of mutant DNA was reproducibly demonstrated.

Graphical abstract: Quantitative detection of single base mutation by combining PNA hybridization and MALDI-TOF mass analysis

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Article information

DOI
https://doi.org/10.1039/C3CC00070B
Article type
Communication
Submitted
04 1 2013
Accepted
18 3 2013
First published
18 3 2013

Chem. Commun., 2013,49, 3754-3756

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Quantitative detection of single base mutation by combining PNA hybridization and MALDI-TOF mass analysis

Y. J. Yeo, K. Roh, J. Y. Bang, E. H. Lee, H. S. Park and D. Kim, Chem. Commun., 2013, 49, 3754 DOI: 10.1039/C3CC00070B

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