Issue 8, 2018

Fluorescent analysis of bioactive molecules in single cells based on microfluidic chips

Abstract

Single-cell analysis of bioactive molecules is an essential strategy for a better understanding of cell biology, exploring cell heterogeneity, and improvement of the ability to detect early diseases. In single-cell analysis, highly efficient single-cell manipulation techniques and high-sensitive detection schemes are in urgent need. The rapid development of fluorescent analysis techniques combined with microfluidic chips have offered a widely applicable solution. Thus, in this review, we mainly focus on the application of fluorescence methods in components analysis on microchips at a single-cell level. By targeting different types of biological molecules in cells such as nucleic acids, proteins, and active small molecules, we specially introduce and comment on their corresponding fluorescent probes, fluorescence labelling and sensing strategies, and different fluorescence detection instruments used in single-cell analysis on a microfluidic chip. We hope that through this review, readers will have a better understanding of single-cell fluorescence analysis, especially for single-cell component fluorescence analysis based on microfluidic chips.

Graphical abstract: Fluorescent analysis of bioactive molecules in single cells based on microfluidic chips

Article information

Article type
Tutorial Review
Submitted
13 12 2017
Accepted
27 2 2018
First published
07 3 2018

Lab Chip, 2018,18, 1151-1173

Fluorescent analysis of bioactive molecules in single cells based on microfluidic chips

Y. Fan, D. Dong, Q. Li, H. Si, H. Pei, L. Li and B. Tang, Lab Chip, 2018, 18, 1151 DOI: 10.1039/C7LC01333G

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