Issue 46, 2014
From the journal:

Chemical Communications

Hoechst tagging: a modular strategy to design synthetic fluorescent probes for live-cell nucleus imaging†‡

Akinobu Nakamura,ab   Kazumasa Takigawa,a   Yasutaka Kurishita,c   Keiko Kuwata,d   Manabu Ishida,e   Yasushi Shimoda,a   Itaru Hamachi*cf  and  Shinya Tsukiji*ae  

* Corresponding authors

a Department of Bioengineering, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata 940-2188, Japan
E-mail: tsukiji@vos.nagaokaut.ac.jp
Fax: +81-258-47-9400
Tel: +81-258-47-9451

b Department of Electrical Engineering, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata 940-2188, Japan

c Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Kyoto 615-8510, Japan
E-mail: ihamachi@sbchem.kyoto-u.ac.jp
Fax: +81-75-383-2759
Tel: +81-75-383-2754

d Institute of Transformative Bio-Molecules (ITbM), Nagoya University, Chikusa, Nagoya 464-8602, Japan

e Top Runner Incubation Center for Academia-Industry Fusion, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata 940-2188, Japan

f Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency, 5 Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan

Abstract

We report a general strategy to create small-molecule fluorescent probes for the nucleus in living cells. Our strategy is based on the attachment of the DNA-binding Hoechst compound to a fluorophore of interest. Using this approach, simple fluorescein, BODIPY, and rhodamine dyes were readily converted to novel turn-on fluorescent nucleus-imaging probes.

Graphical abstract: Hoechst tagging: a modular strategy to design synthetic fluorescent probes for live-cell nucleus imaging

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Article information

DOI
https://doi.org/10.1039/C4CC01753F
Article type
Communication
Submitted
08 3 2014
Accepted
16 4 2014
First published
28 4 2014

Chem. Commun., 2014,50, 6149-6152

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Hoechst tagging: a modular strategy to design synthetic fluorescent probes for live-cell nucleus imaging

A. Nakamura, K. Takigawa, Y. Kurishita, K. Kuwata, M. Ishida, Y. Shimoda, I. Hamachi and S. Tsukiji, Chem. Commun., 2014, 50, 6149 DOI: 10.1039/C4CC01753F

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