Immuno-capture of extracellular vesicles for individual multi-modal characterization using AFM, SEM and Raman spectroscopy

Pepijn Beekman, Agustin Enciso-Martinez, Hoon Suk Rho, Sidharam Pundlik Pujari, Aufried Lenferink, Han Zuilhof, Leon W. M. M. Terstappen, Cees Otto and Séverine Le Gac

Lab Chip, 2019, 19, 2526
DOI: 10.1039/C9LC00081J

Plasmonic nanobowtiefluidic device for sensitive detection of glioma extracellular vesicles by Raman spectrometry

Mahsa Jalali, Imman Isaac Hosseini, Tamer AbdelFatah, Laura Montermini, Sebastian Wachsmann Hogiu, Janusz Rak and Sara Mahshid

Lab Chip, 2021, 21, 855
DOI: 10.1039/D0LC00957A

Microfluidic platforms for extracellular vesicle isolation, analysis and therapy in cancer

Catarina M. Abreu, Bruno Costa-Silva, Rui L. Reis, Subhas C. Kundu and David Caballero

Lab Chip, 2022, 22, 1093
DOI: 10.1039/D2LC00006G

Advances in microfluidic extracellular vesicle analysis for cancer diagnostics

Shibo Cheng, Yutao Li, He Yan, Yunjie Wen, Xin Zhou, Lee Friedman and Yong Zeng

Lab Chip, 2021, 21, 3219
DOI: 10.1039/D1LC00443C

Metal–organic framework-interfaced ELISA probe enables ultrasensitive detection of extracellular vesicle biomarkers

Jingzhu Shi, Sharat Chandra Barman, Shibo Cheng and Yong Zeng

J. Mater. Chem. B, 2024, 12, 6342
DOI: 10.1039/D4TB00585F

A digitalized isothermal nucleic acid testing platform based on a pump-free open droplet array microfluidic chip

Ping Mao, Lei Cao, Zedong Li, Minli You, Bin Gao, Xianghong Xie, Zhenrui Xue, Ping Peng, Chunyan Yao and Feng Xu

Analyst, 2021, 146, 6960
DOI: 10.1039/D1AN01373D

Microfluidic systems for the analysis of blood‐derived molecular biomarkers

Oriana G. Chavez‐Pineda, Roberto Rodriguez‐Moncayo, Diana F. Cedillo‐Alcantar, Pablo E. Guevara‐Pantoja, Josue U. Amador‐Hernandez and Jose L. Garcia‐Cordero

Electrophoresis, 2022, 43, 1667
DOI: 10.1002/elps.202200067

Influence of microfabrication on digital PCR performance in bead-based microwell array assays

Bailey F. McCarthy Riley, Cassandra L. Ward and Thomas H. Linz

Anal Bioanal Chem, 2020, 412, 6917
DOI: 10.1007/s00216-020-02822-2

Latest advances in extracellular vesicles: from bench to bedside

Tomofumi Yamamoto, Nobuyoshi Kosaka and Takahiro Ochiya

Science and Technology of Advanced Materials, 2019, 20, 746
DOI: 10.1080/14686996.2019.1629835

Dodecaborate-Encapsulated Extracellular Vesicles with Modification of Cell-Penetrating Peptides for Enhancing Macropinocytotic Cellular Uptake and Biological Activity in Boron Neutron Capture Therapy

Shiori Hirase, Ayako Aoki, Yoshihide Hattori, Kenta Morimoto, Kosuke Noguchi, Ikuo Fujii, Tomoka Takatani-Nakase, Shiroh Futaki, Mitsunori Kirihata and Ikuhiko Nakase

Mol. Pharmaceutics, 2022, 19, 1135
DOI: 10.1021/acs.molpharmaceut.1c00882

Ultrasensitive detection of mRNA in extracellular vesicles using DNA tetrahedron-based thermophoretic assay

Ziwei Han, Fangning Wan, Jinqi Deng, Junxiang Zhao, Yike Li, Yunjie Yang, Qiao Jiang, Baoquan Ding, Chao Liu, Bo Dai and Jiashu Sun

Nano Today, 2021, 38, 101203
DOI: 10.1016/j.nantod.2021.101203

Circulating Extracellular Vesicle Protein Biomarkers for the Early Detection of High-Grade Serous Ovarian Cancer

Sagar Rayamajhi, Jared Sipes, Bidii Ngala, Amrita Mitra, Meizhang Li, Camille V. Trinidad, Wei Cui, Mohammod Mahmudur Rahman, Foyez Ahmmed, Leonidas E. Bantis, Mihaela E. Sardiu, Dennis W. Province, Harsh B. Pathak and Andrew K. Godwin

Molecular & Cellular Proteomics, 2026, 25, 101508
DOI: 10.1016/j.mcpro.2026.101508

Emerging methods in biomarker identification for extracellular vesicle‐based liquid biopsy

Yaxuan Liang, Brandon M. Lehrich, Siyang Zheng and Mengrou Lu

J of Extracellular Vesicle, 2021, 10
DOI: 10.1002/jev2.12090

Exosomes in sarcomas: Tiny messengers with broad implications in diagnosis, surveillance, prognosis and treatment

Christos Masaoutis, Penelope Korkolopoulou and Stamatios Theocharis

Cancer Letters, 2019, 449, 172
DOI: 10.1016/j.canlet.2019.02.025

Bioprobes-regulated precision biosensing of exosomes: From the nanovesicle surface to the inside

Xin-Xin Peng, Xiaoling Qin, You Qin, Yuanhang Xiang, Guo-Jun Zhang and Fan Yang

Coordination Chemistry Reviews, 2022, 463, 214538
DOI: 10.1016/j.ccr.2022.214538

Exosome-Based Liquid Biopsy Approaches in Bone and Soft Tissue Sarcomas: Review of the Literature, Prospectives, and Hopes for Clinical Application

Chiara Agnoletto, Ymera Pignochino, Chiara Caruso and Cecilia Garofalo

IJMS, 2023, 24, 5159
DOI: 10.3390/ijms24065159

A simple and sensitive direct mRNA multiplexed detection strategy for amoA-targeted monitoring of ammonia-oxidizing activity in water environment

Lan Wang, Jun Wang, Qing Chen, Jian Yang, Liangwei Deng and Zhen Huang

Microchemical Journal, 2021, 162, 105794
DOI: 10.1016/j.microc.2020.105794

Saliva Lab-on-a-chip biosensors: Recent novel ideas and applications in disease detection

Melika Haji Mohammadi, Sterre Mulder, Patricia Khashayar, Alireza Kalbasi, Mostafa Azimzadeh and Amir Reza Aref

Microchemical Journal, 2021, 168, 106506
DOI: 10.1016/j.microc.2021.106506

Microfluidic analysis of circulating tumor cells and tumor-derived extracellular vesicles

Fei Tian, Chao Liu, Ling Lin, Qinghua Chen and Jiashu Sun

TrAC Trends in Analytical Chemistry, 2019, 117, 128
DOI: 10.1016/j.trac.2019.05.013

Recent Advances in Microfluidics for Nucleic Acid Analysis of Small Extracellular Vesicles in Cancer

Sareh Zhand, Dale Mark Goss, Yuen Yee Cheng and Majid Ebrahimi Warkiani

Adv Healthcare Materials, 2025, 14
DOI: 10.1002/adhm.202401295

Beyond tumor mutational burden: potential and limitations in using exosomes to predict response to immunotherapy

Jennifer Crow, Glenson Samuel and Andrew K. Godwin

Expert Review of Molecular Diagnostics, 2019, 19, 1079
DOI: 10.1080/14737159.2020.1688144

The Landscape of Biomimetic Nanovesicles in Brain Diseases

Qing You, Fuming Liang, Gege Wu, Fangfang Cao, Jingyi Liu, Zhaohui He, Chen Wang, Ling Zhu, Xiaoyuan Chen and Yanlian Yang

Advanced Materials, 2024, 36
DOI: 10.1002/adma.202306583

Microfluidic devices for on-chip quantification of extracellular vesicles and associated biomarkers

Víctor Calero, Carlos Honrado, Alar Ainla, Daniela Macedo, Hugo Silva, Teresa Lage, Sara Abalde-Cela, Lorena Diéguez, María Carmen Blanco-López and Esther Serrano-Pertierra

Extracell Vesicles Circ Nucleic Acids., 2025, 6, 560
DOI: 10.20517/evcna.2025.10

MicroRNA Content of Ewing Sarcoma Derived Extracellular Vesicles Leads to Biomarker Potential and Identification of a Previously Undocumented EWS-FLI1 Translocation

Jennifer Crow, Glenson Samuel, Emily Farrow, Margaret Gibson, Jefferey Johnston, Erin Guest, Neil Miller, Dong Pei, Devin Koestler, Harsh Pathak, Xiaobo Liang, Cooper Mangels and Andrew K Godwin

Biomark�Insights, 2022, 17
DOI: 10.1177/11772719221132693

Microfluidics‐based molecular profiling of tumor‐derived exosomes for liquid biopsy

Yuqing Wang, Shurong Wang, Lanting Li, Yan Zou, Baohong Liu and Xiaoni Fang

VIEW, 2023, 4
DOI: 10.1002/VIW.20220048

Application of microfluidics in sports and tumor biomarker detection: Advancing the identification of physiological disorders

Xiaowei Chen, A. Ghorbani, Long Quan and D. Toghraie

Journal of Molecular Liquids, 2023, 387, 122706
DOI: 10.1016/j.molliq.2023.122706

Recent advances of emerging microfluidic chips for exosome mediated cancer diagnosis

Le Wang, Meng-Meng Pan, Li Xu, Xu Yu and Si-Yang Zheng

Smart Materials in Medicine, 2021, 2, 158
DOI: 10.1016/j.smaim.2021.06.001

Hydromechanical Modulation of Enzymatic Kinetics Using Microfluidically Configurable Nanoconfinement Arrays

Yunjie Wen, Yutao Li, Henry C. W. Chu, Shibo Cheng and Yong Zeng

ACS Cent. Sci., 2024, 10, 2059
DOI: 10.1021/acscentsci.4c01094

Ewing sarcoma family of tumors-derived small extracellular vesicle proteomics identify potential clinical biomarkers

Glenson Samuel, Jennifer Crow, Jon B. Klein, Michael L. Merchant, Emily Nissen, Devin C. Koestler, Kris Laurence, Xiaobo Liang, Kathleen Neville, Vincent Staggs, Atif Ahmed, Safinur Atay and Andrew K. Godwin

Oncotarget, 2020, 11, 2995
DOI: 10.18632/oncotarget.27678

Advances in Analytical Technologies for Extracellular Vesicles

He Yan, Yutao Li, Shibo Cheng and Yong Zeng

Anal. Chem., 2021, 93, 4739
DOI: 10.1021/acs.analchem.1c00693

Nanomaterials-Based Exosomes for the Diagnostics and Drug Deliveries of Central Nervous System Diseases

Izzati Haizan, Dong Hyeok Park, Min Yu Choi, Hyerim Lee and Jin-Ha Choi

BioChip J, 2023, 17, 293
DOI: 10.1007/s13206-023-00112-4

Tumor Extracellular Vesicle Digital Scoring Assays for Cancer Detection and Monitoring

Junseok Lee, Chen Zhao, Jacqueline Yang, Steven J. Jonas, Vatche Agopian, Sungyong You, Edwin Posadas, Ju Dong Yang, Yazhen Zhu and Hsian-Rong Tseng

Precision Chemistry, 2025, 3, 662
DOI: 10.1021/prechem.5c00073

Microfluidic systems for cancer diagnostics

Jose L Garcia-Cordero and Sebastian J Maerkl

Current Opinion in Biotechnology, 2020, 65, 37
DOI: 10.1016/j.copbio.2019.11.022

Extracellular Vesicles for Clinical Diagnostics: From Bulk Measurements to Single-Vesicle Analysis

Hai Linh Tran, Wenshu Zheng, David A. Issadore, Hyungsoon Im, Yoon-Kyoung Cho, Yuanqing Zhang, Dingbin Liu, Yang Liu, Bo Li, Fei Liu, David Tai Wai Wong, Jiashu Sun, Kun Qian, Mei He, Meihua Wan, Yong Zeng, Ke Cheng, Tony Jun Huang, Daniel T. Chiu, Luke P. Lee, Lei Zheng, Andrew K. Godwin, Raghu Kalluri, Steven A. Soper and Tony Y. Hu

ACS Nano, 2025, 19, 28021
DOI: 10.1021/acsnano.5c00706

Microfluidic Devices for Manufacture of Therapeutic Extracellular Vesicles: Advances and Opportunities

Amin Hassanzadeh‐Barforoushi, Xenia Sango, Ella L. Johnston, David Haylock and Yuling Wang

J of Extracellular Vesicle, 2025, 14
DOI: 10.1002/jev2.70132

Th4+ tuned aggregation-induced emission: A novel strategy for sequential ultrasensitive detection and separation of Th4+ and Hg2+

You-Ming Zhang, Wei Zhu, Qi Zhao, Wen-Juan Qu, Hong Yao, Tai-Bao Wei and Qi Lin

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2020, 229, 117926
DOI: 10.1016/j.saa.2019.117926

Measurement of mRNA Therapeutics: Method Development and Validation Challenges

Neil Henderson and Amanda Wilson

Bioanalysis, 2019, 11, 2003
DOI: 10.4155/bio-2019-0120

Microfluidic Strategies for Extracellular Vesicle Isolation: Towards Clinical Applications

Alessio Meggiolaro, Valentina Moccia, Paola Brun, Matteo Pierno, Giampaolo Mistura, Valentina Zappulli and Davide Ferraro

Biosensors, 2022, 13, 50
DOI: 10.3390/bios13010050

Ultrafast Detection of Exosomal RNAs via Cationic Lipoplex Nanoparticles in a Micromixer Biochip for Cancer Diagnosis

Yunchen Yang, Eric Kannisto, Santosh K. Patnaik, Mary E. Reid, Lei Li and Yun Wu

ACS Appl. Nano Mater., 2021, 4, 2806
DOI: 10.1021/acsanm.0c03426

Identification of small extracellular vesicle protein biomarkers for pediatric Ewing Sarcoma

Soumya M. Turaga, Mihaela E. Sardiu, Vikalp Vishwakarma, Amrita Mitra, Leonidas E. Bantis, Rashna Madan, Michael L. Merchant, Jon B. Klein, Glenson Samuel and Andrew K. Godwin

Front. Mol. Biosci., 2023, 10
DOI: 10.3389/fmolb.2023.1138594

Recent advances in exosomal RNAs analysis towards diagnostic and therapeutic applications

Shuo Yin, Aipeng Chen, Yue Ding, Jia Song, Rui Chen, Peng Zhang and Chaoyong Yang

TrAC Trends in Analytical Chemistry, 2023, 158, 116840
DOI: 10.1016/j.trac.2022.116840

Coupling Nanostructured Microchips with Covalent Chemistry Enables Purification of Sarcoma‐Derived Extracellular Vesicles for Downstream Functional Studies

Jiantong Dong, Ryan Y. Zhang, Na Sun, Junhui Hu, Matthew D. Smalley, Anqi Zhou, Yue Hua, Winston Rothermich, Mengxiang Chen, Jiayuan Chen, Jinglei Ye, Pai‐Chi Teng, Dongping Qi, Jeffrey A. Toretsky, James S. Tomlinson, Mengyuan Li, Paul S. Weiss, Steven J. Jonas, Noah Federman, Lily Wu, Meiping Zhao, Hsian‐Rong Tseng and Yazhen Zhu

Adv Funct Materials, 2020, 30
DOI: 10.1002/adfm.202003237

Partition-Less Digital Immunoassay Using Configurable Topographic Nanoarrays for Extracellular Vesicle Diagnosis of Ewing Sarcoma

Yunjie Wen, Yutao Li, Shibo Cheng, Jennifer Crow, Glenson Samuel, Vikalp Vishwakarma, Soumya M. Turaga, Leonidas Bantis, Andrew K. Godwin and Yong Zeng

ACS Nano, 2025, 19, 11973
DOI: 10.1021/acsnano.4c16904

Nanotechnology-Inspired Extracellular Vesicles Theranostics for Diagnosis and Therapy of Central Nervous System Diseases

Tian Tian, Shuya Qiao and Bakhos A. Tannous

ACS Appl. Mater. Interfaces, 2023, 15, 182
DOI: 10.1021/acsami.2c07981

Extracellular Vesicles: A New Source of Biomarkers in Pediatric Solid Tumors? A Systematic Review

Nathalie S. M. Lak, Elvera J. van der Kooi, Agustin Enciso-Martinez, Estefanía Lozano-Andrés, Cees Otto, Marca H. M. Wauben and Godelieve A. M. Tytgat

Front. Oncol., 2022, 12
DOI: 10.3389/fonc.2022.887210

Potential of on‐chip analysis and engineering techniques for extracellular vesicle bioproduction for therapeutics

Max Piffoux, Amanda K. A. Silva, Florence Gazeau and Hugo Salmon

VIEW, 2022, 3
DOI: 10.1002/VIW.20200175

Cancer-Derived Extracellular Vesicles: Their Role in Sarcoma

Anita Adib, Ruhi Sahu, Shivangi Mohta, Raphael Etomar Pollock and Lucia Casadei

Life, 2022, 12, 481
DOI: 10.3390/life12040481

Microfluidic systems in extracellular vesicles single analysis. A systematic review

Francisco G. Ortega-Sanchez, Valero Teresa, Thomas Widmann, Matías Regiart, María T. Jerez-Salcedo, Martín A. Fernández-Baldo and Diego de Miguel-Perez

TrAC Trends in Analytical Chemistry, 2023, 159, 116920
DOI: 10.1016/j.trac.2023.116920

Advanced Devices for Tumor Diagnosis and Therapy

Lude Wang, Chendong Dai, Lianfu Jiang, Gangling Tong, Yunhai Xiong, Karim Khan, Zhongmin Tang, Xiang Chen and Haibo Zeng

Small, 2021, 17
DOI: 10.1002/smll.202100003

Sensor-Integrated Microfluidic Approaches for Liquid Biopsies Applications in Early Detection of Cancer

Jessica Sierra, José Marrugo-Ramírez, Romen Rodriguez-Trujillo, Mònica Mir and Josep Samitier

Sensors, 2020, 20, 1317
DOI: 10.3390/s20051317