Issue 1, 2018

Fluorescent probes for the simultaneous detection of multiple analytes in biology

Abstract

Many of the key questions facing cellular biology concern the location and concentration of chemical species, from signalling molecules to metabolites to exogenous toxins. Fluorescent sensors (probes) have revolutionised the understanding of biological systems through their exquisite sensitivity to specific analytes. Probe design has focussed on selective sensors for individual analytes, but many of the most pertinent biological questions are related to the interaction of more than one chemical species. While it is possible to simultaneously use multiple sensors for such applications, data interpretation will be confounded by the fact that sensors will have different uptake, localisation and metabolism profiles. An alternative solution is to instead use a single probe that responds to two analytes, termed a dual-responsive probe. Recent progress in this field has yielded exciting probes, some of which have demonstrated biological application. Here we review work that has been carried out to date, and suggest future research directions that will harness the considerable potential of dual-responsive fluorescent probes.

Graphical abstract: Fluorescent probes for the simultaneous detection of multiple analytes in biology

Article information

Article type
Review Article
Submitted
19 7 2017
First published
09 11 2017

Chem. Soc. Rev., 2018,47, 195-208

Fluorescent probes for the simultaneous detection of multiple analytes in biology

J. L. Kolanowski, F. Liu and E. J. New, Chem. Soc. Rev., 2018, 47, 195 DOI: 10.1039/C7CS00528H

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