Issue 6, 2011

2D IRphoton echo of azido-probes for biomolecular dynamics

Abstract

The vibrations in the azido-, N3, asymmetric stretching region of 2′-azido-2′-deoxyuridine (N3dU) are examined by two-dimensional infrared spectroscopy. In water and tetrahydrofuran (THF), the spectra display a single sharp diagonal peak that shows solvent sensitivity. The frequency–frequency correlation time in water is 1.5 ps, consistent with H-bond making and breaking dynamics. The 2D IR spectrum is reproduced for N3dU in water based on a model correlation function and known linear response functions. Its large extinction coefficient, vibrational frequency outside the protein and nucleic acid IR absorption, and sensitivity to water dynamics render –N3 a very useful probe for 2D IR and other nonlinear IR studies: its signal is ca. 100 times that of nitriles.

Graphical abstract: 2D IR photon echo of azido-probes for biomolecular dynamics

Supplementary files

Article information

Article type
Paper
Submitted
27 8 2010
Accepted
03 11 2010
First published
02 12 2010

Phys. Chem. Chem. Phys., 2011,13, 2237-2241

2D IR photon echo of azido-probes for biomolecular dynamics

M. J. Tucker, X. S. Gai, E. E. Fenlon, S. H. Brewer and R. M. Hochstrasser, Phys. Chem. Chem. Phys., 2011, 13, 2237 DOI: 10.1039/C0CP01625J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements