Issue 2, 2024

Influence of sensor composition on nanoparticle and protein interaction with supported lipid bilayers

Abstract

Supported lipid bilayers are often used as model systems for studying interactions of biological membranes with protein or nanoparticles. A supported lipid bilayer is a phospholipid bilayer built on a solid substrate. The latter is typically made of silica or a metal oxide due to the ease of its formation and range of compatible measurement techniques. Recently, a solvent-assisted method involving supported lipid bilayer formation has allowed the extension of compatible substrate materials to include noble metals such as gold. Here, we examine the influence of substrate composition (SiO2vs. Au) on the interactions between anionic ligand-coated Au nanoparticles or cytochrome c and zwitterionic supported lipid bilayers using quartz crystal microbalance with dissipation monitoring. We find that anionic nanoparticles and cytochrome c have higher adsorption to bilayers formed on Au relative to those on SiO2 substrates. We examine the substrate-dependence of nanoparticle adsorption with DLVO theory and all-atom simulations, and find that the stronger attractive van der Waals and weaker repulsive electrostatic forces between anionic nanoparticles and Au substrates vs. anionic nanoparticles and SiO2 substrates could be responsible for the change in adsorption observed. Our results also indicate that the underlying substrate material influences the degree to which nanoscale analytes interact with supported lipid bilayers. Therefore, interpretation of the supported lipid bilayer model system should be conducted with understanding of support properties.

Graphical abstract: Influence of sensor composition on nanoparticle and protein interaction with supported lipid bilayers

Supplementary files

Article information

Article type
Paper
Submitted
19 6 2023
Accepted
31 8 2023
First published
07 9 2023

Environ. Sci.: Nano, 2024,11, 561-577

Author version available

Influence of sensor composition on nanoparticle and protein interaction with supported lipid bilayers

C. A. Reardon-Lochbaum, R. D. Senanayake, R. Amaro Marquez, K. Trinh, K. N. L. Hoang, T. Rangel Guillen, C. J. Murphy, R. J. Hamers, J. A. Pedersen and R. Hernandez, Environ. Sci.: Nano, 2024, 11, 561 DOI: 10.1039/D3EN00406F

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