Issue 13, 2021

Metabolic labeling probes for interrogation of the host–pathogen interaction

Abstract

Bacterial infections are still one of the leading causes of death worldwide; despite the near-ubiquitous availability of antibiotics. With antibiotic resistance on the rise, there is an urgent need for novel classes of antibiotic drugs. One particularly troublesome class of bacteria are those that have evolved highly efficacious mechanisms for surviving inside the host. These contribute to their virulence by immune evasion, and make them harder to treat with antibiotics due to their residence inside intracellular membrane-limited compartments. This has sparked the development of new chemical reporter molecules and bioorthogonal probes that can be metabolically incorporated into bacteria to provide insights into their activity status. In this review, we provide an overview of several classes of metabolic labeling probes capable of targeting either the peptidoglycan cell wall, the mycomembrane of mycobacteria and corynebacteria, or specific bacterial proteins. In addition, we highlight several important insights that have been made using these metabolic labeling probes.

Graphical abstract: Metabolic labeling probes for interrogation of the host–pathogen interaction

Article information

Article type
Review Article
Submitted
17 12 2020
Accepted
01 3 2021
First published
16 3 2021
This article is Open Access
Creative Commons BY-NC license

Org. Biomol. Chem., 2021,19, 2856-2870

Metabolic labeling probes for interrogation of the host–pathogen interaction

B. J. Ignacio, T. Bakkum, K. M. Bonger, N. I. Martin and S. I. van Kasteren, Org. Biomol. Chem., 2021, 19, 2856 DOI: 10.1039/D0OB02517H

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