Issue 1, 2018

Single-cell analysis by ICP-MS/MS as a fast tool for cellular bioavailability studies of arsenite

Abstract

Single-cell inductively coupled plasma mass spectrometry (SC-ICP-MS) has become a powerful and fast tool to evaluate the elemental composition at a single-cell level. In this study, the cellular bioavailability of arsenite (incubation of 25 and 50 μM for 0–48 h) has been successfully assessed by SC-ICP-MS/MS for the first time directly after re-suspending the cells in water. This procedure avoids the normally arising cell membrane permeabilization caused by cell fixation methods (e.g. methanol fixation). The reliability and feasibility of this SC-ICP-MS/MS approach with a limit of detection of 0.35 fg per cell was validated by conventional bulk ICP-MS/MS analysis after cell digestion and parallel measurement of sulfur and phosphorus.

Graphical abstract: Single-cell analysis by ICP-MS/MS as a fast tool for cellular bioavailability studies of arsenite

Supplementary files

Article information

Article type
Communication
Submitted
09 10 2017
Accepted
13 12 2017
First published
13 12 2017
This article is Open Access
Creative Commons BY license

Metallomics, 2018,10, 73-76

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