Issue 19, 2017

Nucleic acid detection using BRET-beacons based on bioluminescent protein–DNA hybrids

Abstract

Bioluminescent molecular beacons have been developed using a modular design approach that relies on BRET between the bright luciferase NanoLuc and a Cy3 acceptor. While classical molecular beacons are hampered by background fluorescence and scattering, these BRET-beacons allow detection of low pM concentrations of nucleic acids directly in complex media.

Graphical abstract: Nucleic acid detection using BRET-beacons based on bioluminescent protein–DNA hybrids

Supplementary files

Article information

Article type
Communication
Submitted
16 12 2016
Accepted
15 2 2017
First published
15 2 2017
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2017,53, 2862-2865

Nucleic acid detection using BRET-beacons based on bioluminescent protein–DNA hybrids

W. Engelen, K. M. van de Wiel, L. H. H. Meijer, B. Saha and M. Merkx, Chem. Commun., 2017, 53, 2862 DOI: 10.1039/C6CC10032E

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements