Stereoselective chemoenzymatic phytate transformations provide access to diverse inositol phosphate derivatives

Georg Markus Häner, Guizhen Liu, Esther Lange, Nikolaus Jork, Klaus Ditrich, Ralf Greiner, Gabriel Schaaf and Henning J. Jessen

Chem. Sci., 2025, 16, 13459
DOI: 10.1039/D5SC02844B

Semi-enzymatic synthesis and application of 13C-isotopically labelled inositol-(1,4,5)-trisphosphate

Atharva Patharkar, Meike Amma, Jaime Isern, Zoé Chaudron, Angélique Besson-Bard, Valérie Nicolas-Francès, Claire Rosnoblet, David Wendehenne, Peter Schmieder and Dorothea Fiedler

RSC Chem. Biol., 2025, 6, 1426
DOI: 10.1039/D5CB00139K

Inositol pyrophosphate catabolism by three families of phosphatases regulates plant growth and development

Florian Laurent, Simon M. Bartsch, Anuj Shukla, Felix Rico-Resendiz, Daniel Couto, Christelle Fuchs, Joël Nicolet, Sylvain Loubéry, Henning J. Jessen, Dorothea Fiedler, Michael Hothorn and Caroline Gutjahr

PLoS Genet, 2024, 20, e1011468
DOI: 10.1371/journal.pgen.1011468

Biochemical and biophysical characterization of inositol-tetrakisphosphate 1-kinase inhibitors

Martin Y. Ng, Huanchen Wang, Haibo Zhang, Isabel Prucker, Lalith Perera, Ekaterina Goncharova, Antony Wamiru, Henning J. Jessen, Robin E. Stanley, Stephen B. Shears, Ji Luo, Barry R. O'Keefe and Brice A.P. Wilson

Journal of Biological Chemistry, 2025, 301, 108274
DOI: 10.1016/j.jbc.2025.108274

Generation of inositol polyphosphates through a phospholipase C-independent pathway involving carbohydrate and sphingolipid metabolism in Trypanosoma cruzi

Mayara S. Bertolini, Sabrina E. Cline, Miguel A. Chiurillo, Brian S. Mantilla, Aharon Eidex, Logan P. Crowe, Danye Qiu, Henning J. Jessen, Adolfo Saiardi, Roberto Docampo, L. David Sibley and Igor C. Almeida

mBio, 2025, 16
DOI: 10.1128/mbio.03318-24

Analytical methods and tools for studying inositol phosphates

Masatoshi Ito, Shinichi Koguchi, Adolfo Saiardi and Eiichiro Nagata

FEBS Letters, 2025
DOI: 10.1002/1873-3468.70246

The wheat VIH2-3B, a functional PPIP5K controls the localization of fasciclin-like arabinogalactan protein

Anuj Shukla, Reshma Gopal, Riya Ghosh, Ankur Chaudhuri, Kanupriya Agrwal, Rahul Tanwar, Henning Jacob Jessen, Debabrata Laha and Ajay Kumar Pandey

Advances in Biological Regulation, 2025, 97, 101103
DOI: 10.1016/j.jbior.2025.101103

Expanding the Inositol Pyrophosphate Toolbox: Stereoselective Synthesis and Application of PP‐InsP4 Isomers in Plant Signaling

Kevin Ritter, Anne‐Sophie C. Braun, Guizhen Liu, Mengsi Lu, Verena Gaugler, Gabriel Schaaf and Henning Jacob Jessen

Angew Chem Int Ed, 2025, 64
DOI: 10.1002/anie.202507058

Phytate metabolism is mediated by microbial cross-feeding in the gut microbiota

Willem M. De Vos, Minh Nguyen Trung, Mark Davids, Guizhen Liu, Melany Rios-Morales, Henning Jessen, Dorothea Fiedler, Max Nieuwdorp and Thi Phuong Nam Bui

Nat Microbiol, 2024, 9, 1812
DOI: 10.1038/s41564-024-01698-7

Synthesis of a New Purine Analogue Class with Antifungal Activity and Improved Potency against Fungal IP3–4K

Desmarini Desmarini, Daniel Truong, Pooja Sethiya, Guizhen Liu, Bethany Bowring, Henning Jessen, Hue Dinh, Amy K. Cain, Philip E. Thompson and Julianne T. Djordjevic

ACS Infect. Dis., 2025, 11, 940
DOI: 10.1021/acsinfecdis.4c00975

Establishing 18O-Labeled Inositol Phosphates for Quantitative Capillary Electrophoresis-Mass Spectrometry: Fragmentation Pathways and Comparison with 13C-Labeled Analogs

Guizhen Liu, Tobias Dürr-Mayer, Mengsi Lu and Henning J. Jessen

Anal. Chem., 2025, 97, 25282
DOI: 10.1021/acs.analchem.5c05114

Assigning the Absolute Configuration of Inositol Poly- and Pyrophosphates by NMR Using a Single Chiral Solvating Agent

Kevin Ritter, Nikolaus Jork, Anne-Sophie Unmüßig, Maja Köhn and Henning J. Jessen

Biomolecules, 2023, 13, 1150
DOI: 10.3390/biom13071150

The inositol phosphate signalling network in physiology and disease

Seyun Kim, Rashna Bhandari, Charles A. Brearley and Adolfo Saiardi

Trends in Biochemical Sciences, 2024, 49, 969
DOI: 10.1016/j.tibs.2024.08.005

Arg1 from Cryptococcus neoformans lacks PI3 kinase activity and conveys virulence roles via its IP 3-4 kinase activity

Desmarini Desmarini, Guizhen Liu, Henning Jessen, Bethany Bowring, Angela Connolly, Ben Crossett, Julianne Teresa Djordjevic and J. Andrew Alspaugh

mBio, 2024, 15
DOI: 10.1128/mbio.00608-24

CDS2 expression regulates de novo phosphatidic acid synthesis

Daniel M. Collins, Vishnu Janardan, David Barneda, Karen E. Anderson, Izabella Niewczas, Diane Taylor, Danye Qiu, Henning J. Jessen, Andrea F. Lopez-Clavijo, Simon Walker, Padinjat Raghu, Jonathan Clark, Len R. Stephens and Phillip T. Hawkins

Biochemical Journal, 2024, 481, 1449
DOI: 10.1042/BCJ20240456

Conservation of heat stress acclimation by the IPK2-type kinases that control the synthesis of the inositol pyrophosphate 4/6-InsP7 in land plants

Ranjana Yadav, Guizhen Liu, Priyanshi Rana, Naga Jyothi Pullagurla, Danye Qiu, Henning J. Jessen, Debabrata Laha and Bao-Cai Tan

PLoS Genet, 2025, 21, e1011838
DOI: 10.1371/journal.pgen.1011838

Effect of Phytate (InsP6) and Other Inositol-Phosphates (InsP5, InsP4, InsP3, InsP2) on Crystallization of Calcium Oxalate, Brushite, and Hydroxyapatite

Paula Calvó, Antònia Costa-Bauza and Felix Grases

Biomolecules, 2023, 13, 1061
DOI: 10.3390/biom13071061

Phosphorus-specific, liquid chromatography inductively coupled plasma mass spectrometry for analysis of inositol phosphate and inositol pyrophosphate metabolism

Colleen Sprigg, Hayley L. Whitfield, Philip T. Leftwich, Hui-Fen Kuo, Tzyy-Jen Chiou, Adolfo Saiardi, Megan L. Shipton, Andrew M. Riley, Barry V.L. Potter, Dawn Scholey, Emily Burton, Mike R. Bedford and Charles A. Brearley

Biochemical Journal, 2025, 482, 1627
DOI: 10.1042/BCJ20253151

Orchestration of phosphate homeostasis by the ITPK1-type inositol phosphate kinase in the liverwort Marchantia polymorpha

Naga Jyothi Pullagurla, Supritam Shome, Guizhen Liu, Henning J Jessen and Debabrata Laha

Plant Physiology, 2025, 197
DOI: 10.1093/plphys/kiae454

The expanding landscape of inositol phosphate signaling network in land plants

Riya Ghosh, Ranjana Yadav, Naga Jyothi Pullagurla, Priyanshi Rana and Debabrata Laha

FEBS Letters, 2025
DOI: 10.1002/1873-3468.70236

Pools of Independently Cycling Inositol Phosphates Revealed by Pulse Labeling with 18O-Water

Geun-Don Kim, Guizhen Liu, Danye Qiu, Maria Giovanna De Leo, Navin Gopaldass, Jacques Hermes, Jens Timmer, Adolfo Saiardi, Andreas Mayer and Henning Jacob Jessen

J. Am. Chem. Soc., 2025, 147, 17626
DOI: 10.1021/jacs.4c16206

Neue Werkzeuge für die Inositolpyrophosphat‐Forschung: Stereoselektive Synthese und Einsatz von PP‐InsP₄‐Isomeren in der Pflanzenforschung

Kevin Ritter, Anne‐Sophie C. Braun, Guizhen Liu, Mengsi Lu, Verena Gaugler, Gabriel Schaaf and Henning Jacob Jessen

Angewandte Chemie, 2025, 137
DOI: 10.1002/ange.202507058

Bacterial Pathogen Infection Triggers Magic Spot Nucleotide Signaling in Arabidopsis thaliana Chloroplasts through Specific RelA/SpoT Homologues

Danye Qiu, Esther Lange, Thomas M. Haas, Isabel Prucker, Shinji Masuda, Yan L. Wang, Georg Felix, Gabriel Schaaf and Henning J. Jessen

J. Am. Chem. Soc., 2023, 145, 16081
DOI: 10.1021/jacs.3c04445