Integrated biosensors for monitoring microphysiological systems

Lei Mou, Kalpana Mandal, Marvin Magan Mecwan, Ana Lopez Hernandez, Surjendu Maity, Saurabh Sharma, Rondinelli Donizetti Herculano, Satoru Kawakita, Vadim Jucaud, Mehmet Remzi Dokmeci and Ali Khademhosseini

Lab Chip, 2022, 22, 3801
DOI: 10.1039/D2LC00262K

Dynamic measurement of airway surface liquid volume with an ex vivo trachea-chip

Michael Scott, Lei Lei, Kaleb C. Bierstedt, Paul B. McCray and Yuliang Xie

Lab Chip, 2024, 24, 3093
DOI: 10.1039/D4LC00134F

Microfluidic organotypic device to test intestinal mucosal barrier permeability ex vivo

Amanda E. Cherwin, Hayley N. Templeton, Alexis T. Ehrlich, Brielle H. Patlin, Charles S. Henry and Stuart A. Tobet

Lab Chip, 2023, 23, 4126
DOI: 10.1039/D3LC00615H

Development of a microfluidic device to observe dynamic flow around the villi generated by deformation of small intestinal tissue

Satoru Kuriu, Naoyuki Yamamoto and Tadashi Ishida

Lab Chip, 2023, 23, 2729
DOI: 10.1039/D3LC00172E

Lab-on-chip technologies for exploring the gut–immune axis in metabolic disease

Alexandra E. Wheeler, Verena Stoeger and Róisín M. Owens

Lab Chip, 2024, 24, 1266
DOI: 10.1039/D3LC00877K

Advances in Microfluidics: Technical Innovations and Applications in Diagnostics and Therapeutics

Guillaume Aubry, Hyun Jee Lee and Hang Lu

Anal. Chem., 2023, 95, 444
DOI: 10.1021/acs.analchem.2c04562

Advanced epithelial lung and gut barrier models demonstrate passage of microplastic particles

Joanne M. Donkers, Elena M. Höppener, Ilya Grigoriev, Lena Will, Barbro N. Melgert, Bas van der Zaan, Evita van de Steeg and Ingeborg M. Kooter

Micropl.&Nanopl., 2022, 2
DOI: 10.1186/s43591-021-00024-w

Head-to-Head Comparison of Caco-2 Transwell and Gut-on-a-Chip Models for Assessing Oral Peptide Formulations

John P. Gleeson, Stephanie Y. Zhang, Natalia Subelzu, Jing Ling, Becky Nissley, Whitney Ong, Rebecca Nofsinger and Filippos Kesisoglou

Mol. Pharmaceutics, 2024, 21, 3880
DOI: 10.1021/acs.molpharmaceut.4c00210

A hollow fiber membrane-based liver organoid-on-a-chip model for examining drug metabolism and transport

Adam Myszczyszyn, Anna Muench, Vivian Lehmann, Theo Sinnige, Frank G van Steenbeek, Manon Bouwmeester, Roos-Anne Samsom, Marit Keuper-Navis, Thomas K van der Made, Daniel Kogan, Sarah Braem, Luc J W van der Laan, Hossein Eslami Amirabadi, Evita van de Steeg, Rosalinde Masereeuw and Bart Spee

Biofabrication, 2025, 17, 025035
DOI: 10.1088/1758-5090/adc3ce

Challenges in Permeability Assessment for Oral Drug Product Development

Mirko Koziolek, Patrick Augustijns, Constantin Berger, Rodrigo Cristofoletti, David Dahlgren, Janneke Keemink, Pär Matsson, Fiona McCartney, Marco Metzger, Mario Mezler, Janis Niessen, James E. Polli, Maria Vertzoni, Werner Weitschies and Jennifer Dressman

Pharmaceutics, 2023, 15, 2397
DOI: 10.3390/pharmaceutics15102397

A host-microbial metabolite interaction gut-on-a-chip model of the adult human intestine demonstrates beneficial effects upon inulin treatment of gut microbiome

Joanne M. Donkers, Maria Wiese, Tim J. van den Broek, Esmée Wierenga, Valeria Agamennone, Frank Schuren and Evita van de Steeg

Microbiome Res Rep, 2024, 3
DOI: 10.20517/mrr.2023.79

Emerging microfluidic gut-on-a-chip systems for drug development

Xueqi Wang, Yuzhuo Zhu, Zhaoming Cheng, Chuanjun Zhang, Yumeng Liao, Boshi Liu, Di Zhang, Zheng Li and Yuxin Fang

Acta Biomaterialia, 2024, 188, 48
DOI: 10.1016/j.actbio.2024.09.012

Recent Advances in Gut‐ and Gut–Organ‐Axis‐on‐a‐Chip Models

Raehyun Kim and Jong Hwan Sung

Adv Healthcare Materials, 2024, 13
DOI: 10.1002/adhm.202302777

The application of organ-on-chip models for the prediction of human pharmacokinetic profiles during drug development

Marit Keuper-Navis, Markus Walles, Birk Poller, Adam Myszczyszyn, Thomas K. van der Made, Joanne Donkers, Hossein Eslami Amirabadi, Martijn J. Wilmer, Saskia Aan, Bart Spee, Rosalinde Masereeuw and Evita van de Steeg

Pharmacological Research, 2023, 195, 106853
DOI: 10.1016/j.phrs.2023.106853

The application of explants, crypts, and organoids as models in intestinal barrier research

Snezhanna Medvedeva, Kseniya Achasova, Lidiya Boldyreva, Anna Ogienko and Elena Kozhevnikova

Tissue Barriers, 2024
DOI: 10.1080/21688370.2024.2423137

A Linkable, Polycarbonate Gut Microbiome‐Distal Tumor Chip Platform for Interrogating Cancer Promoting Mechanisms

Danielle S.K. Brasino, Sean D. Speese, Kevin Schilling, Carolyn E. Schutt and Michelle C. Barton

Advanced Science, 2024, 11
DOI: 10.1002/advs.202309220

A Critical Review on In Vitro and Ex Vivo Models of the Intestinal Epithelium of Humans and Monogastric Animals

Célia Maria Costa, Nelson Mota de Carvalho, Diana Luazi de Oliveira and Ana Raquel Madureira

Gastrointestinal Disorders, 2024, 6, 337
DOI: 10.3390/gidisord6010023

Validation of a Caco-2 microfluidic Chip model for predicting intestinal absorption of BCS Class I-IV drugs

Stephanie Y. Zhang, Whitney S.Y. Ong, Natalia Subelzu and John P. Gleeson

International Journal of Pharmaceutics, 2024, 656, 124089
DOI: 10.1016/j.ijpharm.2024.124089

Clinical outcomes of best practices for the treatment of distal ulcerative colitis

Nzubechukwu G. Okeke, Eunice K. Omeludike, Eunice T. Aregbesola, Chukwuka Elendu, Babajide T. Obidigbo, Ibukunoluwa E. Oshobu, Papa K.A. Beeko, Ebunoluwa G. Adenuga, Oreoluwa A. Sowunmi, Stephen D. Adetoye, Linda S. Mensah and Richard O. Adetoye

International Journal of Surgery: Global Health, 2025, 8
DOI: 10.1097/GH9.0000000000000523

Microphysiological systems to advance human pathophysiology and translational medicine

Anicca D. Harriot, Christopher W. Ward and Deok-Ho Kim

Journal of Applied Physiology, 2024, 137, 1494
DOI: 10.1152/japplphysiol.00087.2024

Augmented 3D Printing for Multiscale Microphysiological Systems

Kyeong Seob Hwang, Jiyoung Song, Hyun Wook Kang, Jongbaeg Kim, Cheol Woo Ha, Nakwon Choi, Seokyoung Bang and Hong Nam Kim

Small, 2025
DOI: 10.1002/smll.202504750

Modeling of chitosan modified PLGA atorvastatin-curcumin conjugate (AT-CU) nanoparticles, overcoming the barriers associated with PLGA: An approach for better management of atherosclerosis

Rasmita Dash, Madhulika Yadav, Jyotirmaya Biswal, Anshuman Chandra, Vijay Kumar Goel, Tripti Sharma, Shakti Ketan Prusty and Sujata Mohapatra

International Journal of Pharmaceutics, 2023, 640, 123009
DOI: 10.1016/j.ijpharm.2023.123009

Gut-on-a-Chip Research for Drug Development: Implications of Chip Design on Preclinical Oral Bioavailability or Intestinal Disease Studies

Joanne M. Donkers, Jamie I. van der Vaart and Evita van de Steeg

Biomimetics, 2023, 8, 226
DOI: 10.3390/biomimetics8020226

Biomedical Applications of Microfluidic Devices: A Review

Ghazaleh Gharib, İsmail Bütün, Zülâl Muganlı, Gül Kozalak, İlayda Namlı, Seyedali Seyedmirzaei Sarraf, Vahid Ebrahimpour Ahmadi, Erçil Toyran, Andre J. van Wijnen and Ali Koşar

Biosensors, 2022, 12, 1023
DOI: 10.3390/bios12111023

Exploring Toxoplasma gondii´s Biology within the Intestinal Epithelium: intestinal-derived models to unravel sexual differentiation

Florencia Sena, Saira Cancela, Mariela Bollati-Fogolín, Romina Pagotto and María E. Francia

Front. Cell. Infect. Microbiol., 2023, 13
DOI: 10.3389/fcimb.2023.1134471

Microfluidic technologies for ex vivo tissue biopsies: A review

F.C. Garcia-Garcia, P.L. Candarlioglu, J.D. Porter, D.E. Davies, E.J. Swindle and H. Morgan

Organs-on-a-Chip, 2022, 4, 100020
DOI: 10.1016/j.ooc.2022.100020

Intestinal organ chips for disease modelling and personalized medicine

Alican Özkan, Nina Teresa LoGrande, Jessica F. Feitor, Girija Goyal and Donald E. Ingber

Nat Rev Gastroenterol Hepatol, 2024, 21, 751
DOI: 10.1038/s41575-024-00968-3

A microphysiological system for studying barrier health of live tissues in real time

Ryan Way, Hayley Templeton, Daniel Ball, Ming-Hao Cheng, Stuart A. Tobet and Thomas Chen

Commun Eng, 2024, 3
DOI: 10.1038/s44172-024-00285-2

Elevated risk of adverse effects from foodborne contaminants and drugs in inflammatory bowel disease: a review

Tom Walraven, Mathias Busch, Jingxuan Wang, Joanne M. Donkers, Marjolijn Duijvestein, Evita van de Steeg, Nynke I. Kramer and Hans Bouwmeester

Arch Toxicol, 2024, 98, 3519
DOI: 10.1007/s00204-024-03844-w

Human organotypic colon in vitro microtissue: unveiling a new window into colonic drug disposition

Pedro G.M. Canhão, Jan Snoeys, Suzy Geerinckx, Marjolein van Heerden, An Van den Bergh, Camden Holm, Jan Markus, Seyoum Ayehunie, Mario Monshouwer, Raymond Evers, Patrick Augustijns and Stephanie Kourula

European Journal of Pharmaceutical Sciences, 2025, 209, 107025
DOI: 10.1016/j.ejps.2025.107025

Intestinal Cells-on-Chip for Permeability Studies

Marit Keuper-Navis, Hossein Eslami Amirabadi, Joanne Donkers, Markus Walles, Birk Poller, Bo Heming, Lisanne Pieters, Bjorn de Wagenaar, Adam Myszczyszyn, Theo Sinnige, Bart Spee, Rosalinde Masereeuw and Evita van de Steeg

Micromachines, 2024, 15, 1464
DOI: 10.3390/mi15121464