Themed collection Most popular 2018-2019 analytical chemistry articles

The dynamic fluctuations of bioactive species in living cells are associated with numerous physiological and pathological phenomena. The emergence of organelle-targeted fluorescent probes has significantly facilitated our understanding on the biological functions of these species. This review describes the design, applications, challenges and potential directions of organelle-targeted bioactive species probes.

Although there have been substantial improvements in glycan analysis over the past decade, the lack of both high-resolution and high-throughput methods hampers progress in glycomics.

This minireview summarizes the recent achievements of electrochemical imaging platforms to map cellular functions in biological specimens using electrochemical scanning nano/micro-probe microscopy and 2D chips containing microelectrode arrays.

A review of recent advances in near-infrared II fluorophores for multifunctional biomedical imaging.

Two endoplasmic reticulum (ER) targeting probes were developed to image mobile Zn²⁺ to help understand Zn²⁺ related biological processes in the ER.

A “double-locked” and enzyme-activated molecular fluorescent probe for accurate bioimaging and hepatopathy differentiation.

Theranostic nanoprobes (SYL NPs) prepared from a newly designed NIR-II fluorophore and FDA approved DSPE-mPEG5000 were well tolerated and exhibited multifunctional potential in diagnosis (photoacoustic & fluorescence imaging) and phototherapy in animal models.

5BMF is a new fluorescent mitochondria-accumulating delocalized lipophilic cations [DLC] that boasts significantly increased anti-cancer effects and low toxicity in comparison to previous DLCs, addressing current hurdles in DLC clinical translation.

A molecular design strategy is established to access diverse enzyme-activated probes that are excitable and emit in the NIR (I and II) region with favorable Stokes shifts, enabling targeted cancer imaging by real-time monitoring enzyme activities.

Diketopyrrolopyrrole-based fluorescent probes have been developed for the detection of low pH and Zn²⁺ in an AND logic fashion. They can discriminate between cancerous and healthy prostate tissue.

Probe C7 for the first time presents different modes of fluorescence signals to both nanomolar and micromolar concentrations of HOCl.

Telomerase and poly(ADP-ribose) polymerase-1 (PARP-1) are two potential cancer biomarkers and are closely related to tumor initiation and malignant progression.

The first demonstration of direct sequencing of 2′-deoxy-2′-fluoroarabinonucleic acid (FANA) using Nanopore-Induced Phase-Shift Sequencing (NIPSS).

The Unified Compendium is an online interactive tool that utilizes ion mobility collision cross sections to annotate biochemical molecules.

We describe an enzyme-regulated liberation strategy to in situ generate AIEgen nanoaggregates for on-site sensing and long-term tracking of β-galactosidase in ovarian cancer cells.

A super-contrast NIR-II fluorophore IR-BEMC6P with enhanced quantum yield is developed and the excretion mechanism is identified.

This article presents the first work to fundamentally study the effect of steric hindrance in Pdot systems.

Formyl peptide receptors play important biological and therapeutic roles in wound repair and inflammatory diseases.

A series of chiral fluorescent macrocycles display a remarkable combination of both +/– ECD and strong on/off CPL reversible switching upon cation binding and displacement.

Development of molecular probes for the detection of reactive oxygen and nitrogen species (RONS) is important for the pathology and diagnosis of keloid diseases.

The laminated structure of graphene oxide (GO) confers unique interactions with water molecules which may be utilised in a range of applications that require materials with tuneable hygroscopic properties.

A novel target-responsive smart thermometer for translating molecular detection into a temperature test has been developed.

Reactive oxygen species (ROS) scavenging Mn₃O₄ nanozymes effectively protected live mice from ROS-induced ear-inflammation in vivo.

Binary polymer brush microstructures incorporating ratiometric fluorescent pH indicators enable in situ studies of light-activated transmembrane proton transport by proteorhodopsin.

The first small-molecule based αvβ₃-targeted NIR-II/PET dual-modal probes via base-catalyzed thiol-addition chemistry were concisely assembled and evaluated.

We report the high throughput analysis of reaction mixture arrays using methods and data handling routines that were originally developed for biological tissue imaging.

A long-lived complex 13 could selectively bind to dopamine receptors (D1R/D2R) and monitor their internalization in living cells.

We demonstrate the simultaneous detection of human 8-oxoguanine DNA glycosylase 1 and human alkyladenine DNA glycosylase at the single-molecule level.

We report a near-infrared emissive probe which can detect HOCl in vivo by both fluorescence imaging and the naked eye.

The concatenated hybridization chain reaction (C-HCR) was constructed as a versatile and robust tool for signal amplification and intracellular imaging, which was attributed to the synergistic amplification effect between HCR-1 and HCR-2.