Diversify your bonds! The reactivity of preactivated cycloalkynes in the retro-Cope elimination reaction was quantum chemically investigated. Based on our finding, we rationally designed a suite of next-generation reagents.
Cis-fusion of cyclopropane to dibenzocyclooctyne (DMBO) results in high cycloaddition reactivity via enforcing a unique tub-like structure in the transition state that allows tetrazines and azides to approach DMBO from an unhindered face trajectory.
The Nicholas cyclisation was demonstrated to be an alternative synthetic route for bioorthogonal cyclooctyne-sulfamate reagents.
This article provides a comprehensive overview of recent advances in the development of bioorthogonally activated probes for labelling and precise in situ fluorescence imaging both in vitro and in vivo.
The fundamentals of bio-orthogonal click chemistry are investigated, while exploring mechanistic intricacies, demonstrating the adaptability and promise of this methodology.