An innovative and efficient route to the synthesis of metal-based glycoconjugates: proof-of-concept and potential applications

Andrea Pettenuzzo, Diego Montagner, Patrick McArdle and Luca Ronconi

Dalton Trans., 2018, 47, 10721
DOI: 10.1039/C8DT01583J

Effective one-pot multienzyme (OPME) synthesis of monotreme milk oligosaccharides and other sialosides containing 4-O-acetyl sialic acid

Hai Yu, Jie Zeng, Yanhong Li, Vireak Thon, Baojun Shi and Xi Chen

Org. Biomol. Chem., 2016, 14, 8586
DOI: 10.1039/C6OB01706A

Deracemization of 1-phenylethanols in a one-pot process combining Mn-driven oxidation with enzymatic reduction utilizing a compartmentalization technique

Hirofumi Sato, Rei Yamada, Yomi Watanabe, Takaaki Kiryu, Shintaro Kawano, Motohiro Shizuma and Hideya Kawasaki

RSC Adv., 2022, 12, 10619
DOI: 10.1039/D2RA01326F

Identification of the binding roles of terminal and internal glycan epitopes using enzymatically synthesized N-glycans containing tandem epitopes

Zhigang Wu, Yunpeng Liu, Cheng Ma, Lei Li, Jing Bai, Lauren Byrd-Leotis, Yi Lasanajak, Yuxi Guo, Liuqing Wen, He Zhu, Jing Song, Yanhong Li, David A. Steinhauer, David F. Smith, Baohua Zhao, Xi Chen, Wanyi Guan and Peng George Wang

Org. Biomol. Chem., 2016, 14, 11106
DOI: 10.1039/C6OB01982J

Chemoenzymatic synthesis of para-nitrophenol (pNP)-tagged α2–8-sialosides and high-throughput substrate specificity studies of α2–8-sialidases

Nova Tasnima, Hai Yu, Yanhong Li, Abhishek Santra and Xi Chen

Org. Biomol. Chem., 2017, 15, 160
DOI: 10.1039/C6OB02240E

Recent advances in chemoenzymatic synthesis of oligosaccharides and polysaccharides

Tomonari Tanaka

Polym. Chem., 2026, 17, 125
DOI: 10.1039/D5PY00938C

Gram-scale production of sugar nucleotides and their derivatives

Shuang Li, Shuaishuai Wang, Yaqian Wang, Jingyao Qu, Xian-wei Liu, Peng George Wang and Junqiang Fang

Green Chem., 2021, 23, 2628
DOI: 10.1039/D1GC00711D

Converting Pasteurella multocida α2–3-sialyltransferase 1 (PmST1) to a regioselective α2–6-sialyltransferase by saturation mutagenesis and regioselective screening

John B. McArthur, Hai Yu, Jie Zeng and Xi Chen

Org. Biomol. Chem., 2017, 15, 1700
DOI: 10.1039/C6OB02702D

Acceptor-mediated regioselective enzyme catalyzed sialylation: chemoenzymatic synthesis of GAA-7 ganglioside glycan

Hsin-Kai Tseng, Yung-Yu Su, Ting-Wei Chang, Hsin-Chien Liu, Pei-Jhen Li, Pei-Yun Chiang and Chun-Cheng Lin

Chem. Commun., 2021, 57, 3468
DOI: 10.1039/D1CC00653C

ChemInform Abstract: One‐Pot Multienzyme (OPME) Systems for Chemoenzymatic Synthesis of Carbohydrates

Hai Yu and Xi Chen

ChemInform, 2016, 47
DOI: 10.1002/chin.201617251

Chemoenzymatic Synthesis of a Library of Human Milk Oligosaccharides

Zhongying Xiao, Yuxi Guo, Yunpeng Liu, Lei Li, Qing Zhang, Liuqing Wen, Xuan Wang, Shukkoor Muhammed Kondengaden, Zhigang Wu, Jun Zhou, Xuefeng Cao, Xu Li, Cheng Ma and Peng George Wang

J. Org. Chem., 2016, 81, 5851
DOI: 10.1021/acs.joc.6b00478

Chemical Synthesis and Immunological Evaluation of Helicobacter pylori Serotype O6 Tridecasaccharide O‐Antigen Containing a dd‐Heptoglycan

Guangzong Tian, Jing Hu, Chunjun Qin, Lingxin Li, Xiaopeng Zou, Juntao Cai, Peter H. Seeberger and Jian Yin

Angewandte Chemie, 2020, 132, 13464
DOI: 10.1002/ange.202004267

Tools for Studying Glycans: Recent Advances in Chemoenzymatic Glycan Labeling

Aime Lopez Aguilar, Jennie Grace Briard, Linette Yang, Ben Ovryn, Matthew Scott Macauley and Peng Wu

ACS Chem. Biol., 2017, 12, 611
DOI: 10.1021/acschembio.6b01089

Synthesis of Neuraminidase‐Resistant Sialyllactose Mimetics from N‐Acyl Mannosamines using Metabolically Engineered Escherichia coli

Paul Rivollier, Eric Samain, Sylvie Armand, Isabelle Jeacomine, Emeline Richard and Sébastien Fort

Chemistry A European J, 2023, 29
DOI: 10.1002/chem.202301555

A Redox‐Controlled Substrate Engineering Strategy for Site‐Specific Enzymatic Fucosylation

Na Lu, Yun Li, Hui Xia, Kan Zhong, Chenning Jia, Jinfeng Ye, Xianwei Liu, Chang‐Cheng Liu and Hongzhi Cao

Angewandte Chemie, 2022, 134
DOI: 10.1002/ange.202211032

Glyco-Engineering Cell Surfaces by Exo-Enzymatic Installation of GlcNAz and LacNAz Motifs

Fabiola V. De León González, Marie E. Boddington, Joshua M. Kofsky, Martha I. Prindl and Chantelle J. Capicciotti

ACS Chem. Biol., 2024, 19, 629
DOI: 10.1021/acschembio.3c00542

Leloir Glycosyltransferases as Biocatalysts for Chemical Production

Bernd Nidetzky, Alexander Gutmann and Chao Zhong

ACS Catal., 2018, 8, 6283
DOI: 10.1021/acscatal.8b00710

Exploring anomeric glycosylation of phosphoric acid: Optimisation and scope for non-native substrates

Laura Beswick, Sanaz Ahmadipour, Gert-Jan Hofman, Hannah Wootton, Eleni Dimitriou, Jóhannes Reynisson, Robert A. Field, Bruno Linclau and Gavin J. Miller

Carbohydrate Research, 2020, 488, 107896
DOI: 10.1016/j.carres.2019.107896

Toward Automated Enzymatic Synthesis of Oligosaccharides

Liuqing Wen, Garrett Edmunds, Christopher Gibbons, Jiabin Zhang, Madhusudhan Reddy Gadi, Hailiang Zhu, Junqiang Fang, Xianwei Liu, Yun Kong and Peng George Wang

Chem. Rev., 2018, 118, 8151
DOI: 10.1021/acs.chemrev.8b00066

A Kinase‐Independent One‐Pot Multienzyme Cascade for an Expedient Synthesis of Guanosine 5′‐Diphospho‐d‐mannose

Martin Pfeiffer, Dominik Bulfon, Hansjoerg Weber and Bernd Nidetzky

Adv Synth Catal, 2016, 358, 3809
DOI: 10.1002/adsc.201600761

Efficient and Cost-Effective Synthesis of N-Acetyllactosamine by Sequential Modular Enzymatic Cascade Reactions Involving NTP Regeneration

Runmiao Jiao, Liming Zhang, Ran You, Xinlv Peng, Caixia Pei, Bowen Jiang, Meirong Hu, Jianjun Li, Yuguang Du and Eika W. Qian

J. Agric. Food Chem., 2024, 72, 28060
DOI: 10.1021/acs.jafc.4c08638

Cell-Free Synthetic Glycobiology: Designing and Engineering Glycomolecules Outside of Living Cells

Thapakorn Jaroentomeechai, May N. Taw, Mingji Li, Alicia Aquino, Ninad Agashe, Sean Chung, Michael C. Jewett and Matthew P. DeLisa

Front. Chem., 2020, 8
DOI: 10.3389/fchem.2020.00645

Conjugation of Human N‐Glycans Improves the Drug Properties of Existing Peptides and Proteins

Yuji Nishiuchi, Sofia Elouali, Masato Noguchi and Hirofumi Ochiai

ChemBioChem, 2025, 26
DOI: 10.1002/cbic.202401066

Recent progress in fucosylated derivatives of lacto- N -tetraose and lacto- N -neotetraose

Longhao Yang, Yingying Zhu, Jiawei Meng, Wenli Zhang and Wanmeng Mu

Critical Reviews in Food Science and Nutrition, 2024, 64, 10384
DOI: 10.1080/10408398.2023.2224431

Functional design of glycan-conjugated molecules using a chemoenzymatic approach

Makoto Ogata

Bioscience, Biotechnology, and Biochemistry, 2021, 85, 1046
DOI: 10.1093/bbb/zbab024

Small tools for sweet challenges: advances in microfluidic technologies for glycan synthesis

Ferra Pinnock and Susan Daniel

Anal Bioanal Chem, 2022, 414, 5139
DOI: 10.1007/s00216-022-03948-1

Physiological effects, biosynthesis, and derivatization of key human milk tetrasaccharides, lacto-N-tetraose, and lacto-N-neotetraose

Yingying Zhu, Guocong Luo, Li Wan, Jiawei Meng, Sang Yup Lee and Wanmeng Mu

Critical Reviews in Biotechnology, 2021, 1
DOI: 10.1080/07388551.2021.1944973

Chemical, enzymatic and biological synthesis of hyaluronic acids

Jingmin Li, Meng Qiao, Yuan Ji, Lei Lin, Xing Zhang and Robert J. Linhardt

International Journal of Biological Macromolecules, 2020, 152, 199
DOI: 10.1016/j.ijbiomac.2020.02.214

Development of Diverse Range of Biologically Relevant Carbohydrate‐Containing Molecules: Twenty Years of Our Journey**

Vinod K. Tiwari

The Chemical Record, 2021, 21, 3029
DOI: 10.1002/tcr.202100058

Chemical Evolution of Enzyme-Catalyzed Glycosylation

Chang-Cheng Liu, Jinfeng Ye and Hongzhi Cao

Acc. Chem. Res., 2024
DOI: 10.1021/acs.accounts.3c00754

Chemoenzymatic Synthesis of DSGb5 and Sialylated Globo‐series Glycans

Pei‐Jhen Li, Szu‐Yu Huang, Pei‐Yun Chiang, Chen‐Yo Fan, Li‐Jhen Guo, Dung‐Yeh Wu, Takashi Angata and Chun‐Cheng Lin

Angew Chem Int Ed, 2019, 58, 11273
DOI: 10.1002/anie.201903943

Reprogramming the enzymatic assembly line for site-specific fucosylation

Jinfeng Ye, Hui Xia, Na Sun, Chang-Cheng Liu, Anran Sheng, Lianli Chi, Xian-Wei Liu, Guofeng Gu, Shu-Qi Wang, Jie Zhao, Ping Wang, Min Xiao, Fengshan Wang and Hongzhi Cao

Nat Catal, 2019, 2, 514
DOI: 10.1038/s41929-019-0281-z

Multi-enzyme systems and recombinant cells for synthesis of valuable saccharides: Advances and perspectives

Jiangang Yang, Tong Zhang, Chaoyu Tian, Yueming Zhu, Yan Zeng, Yan Men, Peng Chen, Yuanxia Sun and Yanhe Ma

Biotechnology Advances, 2019, 37, 107406
DOI: 10.1016/j.biotechadv.2019.06.005

Cell-based glycan arrays for probing glycan–glycan binding protein interactions

Jennie Grace Briard, Hao Jiang, Kelley W. Moremen, Matthew Scott Macauley and Peng Wu

Nat Commun, 2018, 9
DOI: 10.1038/s41467-018-03245-5

Facile Chemoenzymatic Synthesis of O‐Mannosyl Glycans

Shuaishuai Wang, Qing Zhang, CongCong Chen, Yuxi Guo, Madhusudhan Reddy Gadi, Jin Yu, Ulrika Westerlind, Yunpeng Liu, Xuefeng Cao, Peng G. Wang and Lei Li

Angewandte Chemie, 2018, 130, 9412
DOI: 10.1002/ange.201803536

Unleashing the Power of Domino Reactions on Carbohydrates: State of the Art

Giulia Antonini, Laura Petrosilli, Ilaria Giussani, Maria Gessica Ciulla, Sarah Mazzotta and Giuseppe D'Orazio

Eur J Org Chem, 2024, 27
DOI: 10.1002/ejoc.202400511

Galectin–Carbohydrate Interactions in Biomedicine and Biotechnology

Dominic Laaf, Pavla Bojarová, Lothar Elling and Vladimír Křen

Trends in Biotechnology, 2019, 37, 402
DOI: 10.1016/j.tibtech.2018.10.001

Pathophysiological roles and applications of glycosphingolipids in the diagnosis and treatment of cancer diseases

Xuefeng Jin and Guang-Yu Yang

Progress in Lipid Research, 2023, 91, 101241
DOI: 10.1016/j.plipres.2023.101241

Substrate and Process Engineering for Biocatalytic Synthesis and Facile Purification of Human Milk Oligosaccharides

Yuanyuan Bai, Xiaohong Yang, Hai Yu and Xi Chen

ChemSusChem, 2022, 15
DOI: 10.1002/cssc.202102539

Cascade synthesis of uridine-5′-diphosphate glucuronic acid by coupling multiple whole cells expressing hyperthermophilic enzymes

Dan-Hua Meng, Ran-Ran Du, Lu-Zhou Chen, Meng-Ting Li, Fei Liu, Jin Hou, Yi-Kang Shi, Feng-Shan Wang and Ju-Zheng Sheng

Microb Cell Fact, 2019, 18
DOI: 10.1186/s12934-019-1168-z

Recent development on stereoselective intramolecular O-glycosylation methodology

Anindra Sharma, Manoj K. Jaiswal, Mangal S. Yadav, Danish Ansari, Rama P. Tripathi and Vinod K. Tiwari

Carbohydrate Research, 2025, 552, 109415
DOI: 10.1016/j.carres.2025.109415

Production of Arabidopsis thaliana UDP-Sugar Pyrophosphorylase by Pichia pastoris and Its Application in Efficient UDP-Glucose and UDP-Glucuronic Acid Synthesis

L. X. Zang, R. R. Du, H. C. Zang, F. S. Wang and J. Z. Sheng

Appl Biochem Microbiol, 2019, 55, 631
DOI: 10.1134/S0003683819060152

Engineering analysis of multienzyme cascade reactions for 3ʹ‐sialyllactose synthesis

Sabine Schelch, Manuel Eibinger, Stefanie Gross Belduma, Barbara Petschacher, Jürgen Kuballa and Bernd Nidetzky

Biotech & Bioengineering, 2021, 118, 4290
DOI: 10.1002/bit.27898

Synthesis of α-Deoxymono and Difluorohexopyranosyl 1-Phosphates and Kinetic Evaluation with Thymidylyl- and Guanidylyltransferases

Jian-She Zhu, Nicole E. McCormick, Shannon C. Timmons and David L. Jakeman

J. Org. Chem., 2016, 81, 8816
DOI: 10.1021/acs.joc.6b01485

Chemoenzymatic Total Synthesis of Haemophilus ducreyi Lipooligosaccharide Core Octasaccharides Containing Natural and Unnatural Sialic Acids

Shiwei Luo, Yating Liu, Tianhui Hao, Wenjing Ma, Yawen Luo, Shasha Wang, Zhuojia Xu, Chaoyu Hu, Liuqing Wen and Tiehai Li

Org. Lett., 2023, 25, 2312
DOI: 10.1021/acs.orglett.3c00657

A Diversity-Oriented Strategy for Chemoenzymatic Synthesis of Glycosphingolipids and Related Derivatives

Qingjiang Li, Mohit Jaiswal, Rajendra S. Rohokale and Zhongwu Guo

Org. Lett., 2020, 22, 8245
DOI: 10.1021/acs.orglett.0c02847

Post-Glycosylation Diversification (PGD): An Approach for Assembling Collections of Glycosylated Small Molecules

Zachary Cannone, Ala M. Shaqra, Chris Lorenc, Liza Henowitz, Santosh Keshipeddy, Victoria L. Robinson, Adam Zweifach, Dennis Wright and Mark W. Peczuh

ACS Comb. Sci., 2019, 21, 192
DOI: 10.1021/acscombsci.8b00139

Oligosaccharide Synthesis and Translational Innovation

Larissa Krasnova and Chi-Huey Wong

J. Am. Chem. Soc., 2019, 141, 3735
DOI: 10.1021/jacs.8b11005

Cell‐Free Multi‐Enzyme Synthesis and Purification of Uridine Diphosphate Galactose

Reza Mahour, Ju Weon Lee, Pia Grimpe, Simon Boecker, Valerian Grote, Steffen Klamt, Andreas Seidel‐Morgenstern, Thomas F. T. Rexer and Udo Reichl

ChemBioChem, 2022, 23
DOI: 10.1002/cbic.202100361

Chemoenzymatic Synthesis of Globo‐series Glycosphingolipids and Evaluation of Their Immunosuppressive Activities

Pei‐Yun Chiang, Avijit K. Adak, Wei‐Lun Liang, Chen‐Yen Tsai, Hsin‐Kai Tseng, Jing‐Yan Cheng, Jih Ru Hwu, Alice L. Yu, Jung‐Tung Hung and Chun‐Cheng Lin

Chemistry An Asian Journal, 2022, 17
DOI: 10.1002/asia.202200403

Recent progress in the synthesis of glycosphingolipids

Hiromune Ando and Naoko Komura

Current Opinion in Chemical Biology, 2024, 78, 102423
DOI: 10.1016/j.cbpa.2023.102423

Glycosylation-on-a-Chip: A Flow-Based Microfluidic System for Cell-Free Glycoprotein Biosynthesis

Alicia K. Aquino, Zachary A. Manzer, Susan Daniel and Matthew P. DeLisa

Front. Mol. Biosci., 2021, 8
DOI: 10.3389/fmolb.2021.782905

Enzyme cascades for high-yield conversion of d-xylose into d-ribose by overcoming equilibrium constraints and enhancing selectivity

Ja Hyun Lee, Doyeon Kim, Yoonjoo Kim, Dong Hyun Kim, Yong-Cheol Park and Kyoung Heon Kim

Bioresource Technology, 2025, 428, 132435
DOI: 10.1016/j.biortech.2025.132435

Automated Glycan Assembly: A Perspective

Mónica Guberman and Peter H. Seeberger

J. Am. Chem. Soc., 2019, 141, 5581
DOI: 10.1021/jacs.9b00638

An Enzymatic N‐Acylation Step Enables the Biocatalytic Synthesis of Unnatural Sialosides

Pedro Laborda, Yong‐Mei Lyu, Fabio Parmeggiani, Ai‐Min Lu, Wen‐Jiao Wang, Ying‐Ying Huang, Kun Huang, Juan Guo, Li Liu, Sabine L. Flitsch and Josef Voglmeir

Angew Chem Int Ed, 2020, 59, 5308
DOI: 10.1002/anie.201914338

Recent Progress in Chemo-Enzymatic Methods for the Synthesis of N-Glycans

Qiang Chao, Yi Ding, Zheng-Hui Chen, Meng-Hai Xiang, Ning Wang and Xiao-Dong Gao

Front. Chem., 2020, 8
DOI: 10.3389/fchem.2020.00513

Biocatalytic Cascade of Polyphosphate Kinase and Sucrose Synthase for Synthesis of Nucleotide‐Activated Derivatives of Glucose

Sandra T. Kulmer, Alexander Gutmann, Martin Lemmerer and Bernd Nidetzky

Adv Synth Catal, 2017, 359, 292
DOI: 10.1002/adsc.201601078

Sugar Auxiliary Group Assisted Diversity‐Oriented Enzymatic Modular Synthesis of 0‐Series Ganglioside Glycans

Jinfeng Ye, Kan Zhong, Zhi‐Fei Hu, Chang‐Cheng Liu, Ming Li, Peng Wu and Hongzhi Cao

Angew Chem Int Ed, 2025, 64
DOI: 10.1002/anie.202418929

Sugar nucleotide regeneration system for the synthesis of Bi- and triantennary N-glycans and exploring their activities against siglecs

Mohammed Tarique Anwar, Sachin Kisan Kawade, Yi-Ren Huo, Avijit K. Adak, Deepa Sridharan, Yan-Ting Kuo, Chen-Yo Fan, Hsin-Ru Wu, Yun-Sheng Lee, Takashi Angata and Chun-Cheng Lin

European Journal of Medicinal Chemistry, 2022, 232, 114146
DOI: 10.1016/j.ejmech.2022.114146

Spatially Segregated MOF Bioreactor Enables Versatile Modular Glycoenzyme Assembly for Hierarchical Glycan Library Construction

Jie Zheng, Han Xu, Bingzhi Li, Muhammad Sohail, Jingjing Bi, Fuming Zhang, Robert J. Linhardt, He Huang and Xing Zhang

ACS Appl. Mater. Interfaces, 2023, 15, 19807
DOI: 10.1021/acsami.2c22094

A concise chemoenzymatic total synthesis of neutral Globo-series glycosphingolipids Globo A and Globo B, and Forssman and para-Forssman antigens

Yu-Ching Chiang, Chun-Yen Wu, Pei-Yun Chiang, Avijit K. Adak and Chun-Cheng Lin

Glycoconj J, 2023, 40, 551
DOI: 10.1007/s10719-023-10133-8

Facile Chemoenzymatic Synthesis of O‐Mannosyl Glycans

Shuaishuai Wang, Qing Zhang, CongCong Chen, Yuxi Guo, Madhusudhan Reddy Gadi, Jin Yu, Ulrika Westerlind, Yunpeng Liu, Xuefeng Cao, Peng G. Wang and Lei Li

Angew Chem Int Ed, 2018, 57, 9268
DOI: 10.1002/anie.201803536

Reprint of: Advanced glycosidases as ingenious biosynthetic instruments

Zuzana Mészáros, Pavlína Nekvasilová, Pavla Bojarová, Vladimír Křen and Kristýna Slámová

Biotechnology Advances, 2021, 51, 107820
DOI: 10.1016/j.biotechadv.2021.107820

Establishment of a five-enzyme cell-free cascade for the synthesis of uridine diphosphate N-acetylglucosamine

Reza Mahour, Jan Klapproth, Thomas F.T. Rexer, Anna Schildbach, Steffen Klamt, Markus Pietzsch, Erdmann Rapp and Udo Reichl

Journal of Biotechnology, 2018, 283, 120
DOI: 10.1016/j.jbiotec.2018.07.027

Sequential One-Pot Multienzyme Chemoenzymatic Synthesis of Glycosphingolipid Glycans

Hai Yu, Yanhong Li, Jie Zeng, Vireak Thon, Dung M. Nguyen, Thao Ly, Hui Yu Kuang, Alice Ngo and Xi Chen

J. Org. Chem., 2016, 81, 10809
DOI: 10.1021/acs.joc.6b01905

Tools for mammalian glycoscience research

Matthew E. Griffin and Linda C. Hsieh-Wilson

Cell, 2022, 185, 2657
DOI: 10.1016/j.cell.2022.06.016

An Automation Platform for the Chemoenzymatic Synthesis of Complex Sulfated and Branched Glycans

Saptashwa Chakraborty, Kyle Minder, Anthony Robert Prudden and Geert-Jan Boons

J. Am. Chem. Soc., 2026, 148, 11020
DOI: 10.1021/jacs.5c22181

Advanced glycosidases as ingenious biosynthetic instruments

Zuzana Mészáros, Pavlína Nekvasilová, Pavla Bojarová, Vladimír Křen and Kristýna Slámová

Biotechnology Advances, 2021, 49, 107733
DOI: 10.1016/j.biotechadv.2021.107733

The Shewanella woodyi galactokinase pool phosphorylates glucose at the 6-position

Louis Patrick Conway, Fang Fang Liu, Qian Li and Josef Voglmeir

Carbohydrate Research, 2018, 455, 39
DOI: 10.1016/j.carres.2017.10.018

Chemoenzymatic Synthesis of DSGb5 and Sialylated Globo‐series Glycans

Pei‐Jhen Li, Szu‐Yu Huang, Pei‐Yun Chiang, Chen‐Yo Fan, Li‐Jhen Guo, Dung‐Yeh Wu, Takashi Angata and Chun‐Cheng Lin

Angewandte Chemie, 2019, 131, 11395
DOI: 10.1002/ange.201903943

Analysis of Synthetic Monodisperse Polysaccharides by Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry

Simone Nicolardi, A. Abragam Joseph, Qian Zhu, Zhengnan Shen, Alonso Pardo-Vargas, Fabrizio Chiodo, Antonio Molinaro, Alba Silipo, Yuri E. M. van der Burgt, Biao Yu, Peter H. Seeberger and Manfred Wuhrer

Anal. Chem., 2021, 93, 4666
DOI: 10.1021/acs.analchem.1c00239

Biocatalytic Approaches to Building Blocks for Enzymatic and Chemical Glycan Synthesis

Jonathan P. Dolan, Sebastian C. Cosgrove and Gavin J. Miller

JACS Au, 2023, 3, 47
DOI: 10.1021/jacsau.2c00529

Sugar Auxiliary Group Assisted Diversity‐Oriented Enzymatic Modular Synthesis of 0‐Series Ganglioside Glycans

Jinfeng Ye, Kan Zhong, Zhi‐Fei Hu, Chang‐Cheng Liu, Ming Li, Peng Wu and Hongzhi Cao

Angewandte Chemie, 2025, 137
DOI: 10.1002/ange.202418929

Recent advances in chemoenzymatic synthesis of human ABO blood group antigens

Jing Liu, Xianwei Liu and Wenyuan Fang

Biotechnology Advances, 2025, 82, 108599
DOI: 10.1016/j.biotechadv.2025.108599

Chemoenzymatic synthesis and biological evaluation of ganglioside GM3 and lyso-GM3 as potential agents for cancer therapy

Tingshen Li, Xiaodan Wang, Peijie Dong, Peng Yu, Yongmin Zhang and Xin Meng

Carbohydrate Research, 2021, 509, 108431
DOI: 10.1016/j.carres.2021.108431

Enzymatic and chemoenzymatic synthesis of human milk oligosaccharides and derivatives

Jie Zheng, Han Xu, Junqiang Fang and Xing Zhang

Carbohydrate Polymers, 2022, 291, 119564
DOI: 10.1016/j.carbpol.2022.119564

A Redox‐Controlled Substrate Engineering Strategy for Site‐Specific Enzymatic Fucosylation

Na Lu, Yun Li, Hui Xia, Kan Zhong, Chenning Jia, Jinfeng Ye, Xianwei Liu, Chang‐Cheng Liu and Hongzhi Cao

Angew Chem Int Ed, 2022, 61
DOI: 10.1002/anie.202211032

An Enzymatic N‐Acylation Step Enables the Biocatalytic Synthesis of Unnatural Sialosides

Pedro Laborda, Yong‐Mei Lyu, Fabio Parmeggiani, Ai‐Min Lu, Wen‐Jiao Wang, Ying‐Ying Huang, Kun Huang, Juan Guo, Li Liu, Sabine L. Flitsch and Josef Voglmeir

Angewandte Chemie, 2020, 132, 5346
DOI: 10.1002/ange.201914338

Enzymatic synthesis of human blood group P1 pentasaccharide antigen

Dawa Tsering, Congcong Chen, Jinfeng Ye, Zhipeng Han, Bai-qian Jing, Xian-wei Liu, Xi Chen, Fengshan Wang, Peixue Ling and Hongzhi Cao

Carbohydrate Research, 2017, 438, 39
DOI: 10.1016/j.carres.2016.11.019

Enzyme cascades for the synthesis of nucleotide sugars: Updates to recent production strategies

Hannes Frohnmeyer and Lothar Elling

Carbohydrate Research, 2023, 523, 108727
DOI: 10.1016/j.carres.2022.108727

Facile and Stereo-Selective Synthesis of UDP-α-D-xylose and UDP-β-L-arabinose Using UDP-Sugar Pyrophosphorylase

JiaJia Wang, Harmon Greenway, Shanshan Li, Mohui Wei, Samuel J. Polizzi and Peng G. Wang

Front. Chem., 2018, 6
DOI: 10.3389/fchem.2018.00163

Production of functional mimics of human milk oligosaccharides by enzymatic glycosylation of bovine milk oligosaccharides

Valerie Weinborn, Yanhong Li, Ishita M. Shah, Hai Yu, David C. Dallas, J. Bruce German, David A. Mills, Xi Chen and Daniela Barile

International Dairy Journal, 2020, 102, 104583
DOI: 10.1016/j.idairyj.2019.104583

Chemical Synthesis and Immunological Evaluation of Helicobacter pylori Serotype O6 Tridecasaccharide O‐Antigen Containing a dd‐Heptoglycan

Guangzong Tian, Jing Hu, Chunjun Qin, Lingxin Li, Xiaopeng Zou, Juntao Cai, Peter H. Seeberger and Jian Yin

Angew Chem Int Ed, 2020, 59, 13362
DOI: 10.1002/anie.202004267

Bacterial sialyltransferases and their use in biocatalytic cascades for sialo-oligosaccharide production

Sabine Schelch, Chao Zhong, Barbara Petschacher and Bernd Nidetzky

Biotechnology Advances, 2020, 44, 107613
DOI: 10.1016/j.biotechadv.2020.107613

Acid-Catalyzed O-Glycosylation with Stable Thioglycoside Donors

Kristina D. Lacey, Rashanique D. Quarels, Shaofu Du, Ashley Fulton, Nicholas J. Reid, Austin Firesheets and Justin R. Ragains

Org. Lett., 2018, 20, 5181
DOI: 10.1021/acs.orglett.8b02125

Synthetic Carbohydrate Chemistry and Translational Medicine

Sachin S. Shivatare and Chi-Huey Wong

J. Org. Chem., 2020, 85, 15780
DOI: 10.1021/acs.joc.0c01834

Application of Biocatalysis to on‐DNA Carbohydrate Library Synthesis

Baptiste Thomas, Xiaojie Lu, William R. Birmingham, Kun Huang, Peter Both, Juana Elizabeth Reyes Martinez, Robert J. Young, Christopher P. Davie and Sabine L. Flitsch

ChemBioChem, 2017, 18, 858
DOI: 10.1002/cbic.201600678

Carbohydrate Kinases: A Conserved Mechanism Across Differing Folds

Sumita Roy, Mirella Vivoli Vega and Nicholas J. Harmer

Catalysts, 2019, 9, 29
DOI: 10.3390/catal9010029

Total Synthesis of Polysaccharides by Automated Glycan Assembly

A. Abragam Joseph, Alonso Pardo-Vargas and Peter H. Seeberger

J. Am. Chem. Soc., 2020, 142, 8561
DOI: 10.1021/jacs.0c00751

MPTGs: Thioglycoside Donors for Acid-Catalyzed O-Glycosylation and Latent-Active Synthetic Strategies

Shaofu Du and Justin R. Ragains

Org. Lett., 2019, 21, 980
DOI: 10.1021/acs.orglett.8b03958

Glycosyltransferases as versatile tools to study the biology of glycans

Joshua M Kofsky, Jonathan L Babulic, Marie E Boddington, Fabiola V De León González and Chantelle J Capicciotti

Glycobiology, 2023, 33, 888
DOI: 10.1093/glycob/cwad092

Fabricating approaches for synthesis of miktoarm star-shaped polymers having tailored biodegradability

Siddhi S. Panchal and Dilip V. Vasava

International Journal of Polymeric Materials and Polymeric Biomaterials, 2022, 71, 1407
DOI: 10.1080/00914037.2021.1981319

Synthetic Strategies for Modified Glycosphingolipids and Their Design as Probes

Carmanah D. Hunter, Tianlin Guo, Gour Daskhan, Michele R. Richards and Christopher W. Cairo

Chem. Rev., 2018, 118, 8188
DOI: 10.1021/acs.chemrev.8b00070

Strategies for chemoenzymatic synthesis of carbohydrates

Wanqing Li, John B. McArthur and Xi Chen

Carbohydrate Research, 2019, 472, 86
DOI: 10.1016/j.carres.2018.11.014

Discovery and Biotechnological Exploitation of Glycoside-Phosphorylases

Ao Li, Mounir Benkoulouche, Simon Ladeveze, Julien Durand, Gianluca Cioci, Elisabeth Laville and Gabrielle Potocki-Veronese

IJMS, 2022, 23, 3043
DOI: 10.3390/ijms23063043

Chemoenzymatic synthesis of tumor-associated antigen N3 minor octasaccharide

Zhipeng Han, Congcong Chen, Caicai Meng, Tian Gao, Peng Peng, Xi Chen, Fengshan Wang and Hongzhi Cao

Journal of Carbohydrate Chemistry, 2016, 35, 412
DOI: 10.1080/07328303.2017.1315123

Strategies for synthesizing human milk lacto-N-fucopentaoses oligosaccharides

Minjiao Zhang, Suyue Lu, Yihan Chen, Xue Wu, Zexu Zhao and Chao Zhao

Journal of Agriculture and Food Research, 2023, 14, 100724
DOI: 10.1016/j.jafr.2023.100724

A General Chemoenzymatic Strategy for the Synthesis of Glycosphingolipids

Yunpeng Liu, Liuqing Wen, Lei Li, Madhusudhan Reddy Gadi, Wanyi Guan, Kenneth Huang, Zhongying Xiao, Mohui Wei, Cheng Ma, Qing Zhang, Hai Yu, Xi Chen, Peng George Wang and Junqiang Fang

Eur J Org Chem, 2016, 2016, 4315
DOI: 10.1002/ejoc.201600950

Recent progress in synthesis of carbohydrates with sugar nucleotide-dependent glycosyltransferases

Lan Na, Riyao Li and Xi Chen

Current Opinion in Chemical Biology, 2021, 61, 81
DOI: 10.1016/j.cbpa.2020.10.007

Chemoenzymatic Synthesis and Facile Purification of Gangliosides

Yuanyuan Bai, Hai Yu and Xi Chen

Current Protocols, 2021, 1
DOI: 10.1002/cpz1.91

Digital microfluidics-engaged automated enzymatic degradation and synthesis of oligosaccharides

Yunze Sun, Yiran Wu, Dachuan Ma, Jian-Jun Li, Xianming Liu, Yuanjiang You, Jun Lu, Zhen Liu, Xin Cheng and Yuguang Du

Front. Bioeng. Biotechnol., 2023, 11
DOI: 10.3389/fbioe.2023.1201300

Editors’ Choice—Perspective—Deciphering the Glycan Kryptos by Solid-State Nanopore Single-Molecule Sensing: A Call for Integrated Advancements Across Glyco- and Nanopore Science

Megan E. Kizer and Jason R. Dwyer

ECS Sens. Plus, 2024, 3, 020604
DOI: 10.1149/2754-2726/ad49b0

Three‐Enzyme Phosphorylase Cascade Immobilized on Solid Support for Biocatalytic Synthesis of Cello−oligosaccharides

Chao Zhong, Božidar Duić, Juan M. Bolivar and Bernd Nidetzky

ChemCatChem, 2020, 12, 1350
DOI: 10.1002/cctc.201901964

Chemoenzymatic Approach for the Preparation of Asymmetric Bi-, Tri-, and Tetra-Antennary N-Glycans from a Common Precursor

Ivan A. Gagarinov, Tiehai Li, Javier Sastre Toraño, Tomislav Caval, Apoorva D. Srivastava, John A. W. Kruijtzer, Albert J. R. Heck and Geert-Jan Boons

J. Am. Chem. Soc., 2017, 139, 1011
DOI: 10.1021/jacs.6b12080

Redox-Controlled Site-Specific α2–6-Sialylation

Na Lu, Jinfeng Ye, Jiansong Cheng, Aniruddha Sasmal, Chang-Cheng Liu, Wenlong Yao, Jun Yan, Naazneen Khan, Wen Yi, Ajit Varki and Hongzhi Cao

J. Am. Chem. Soc., 2019, 141, 4547
DOI: 10.1021/jacs.9b00044

Liquid-Phase and Ultrahigh-Frequency-Acoustofluidics-Based Solid-Phase Synthesis of Biotin-Tagged 6′/3′-Sialyl-N-Acetylglucosamine by Sequential One-Pot Multienzyme System

Mengge Gong, Tiechuan Li, Lina Wu, Zhenxing Zhang, Lishi Ren, Xuexin Duan, Hongzhi Cao, Meishan Pei, Jian-Jun Li and Yuguang Du

Catalysts, 2020, 10, 1347
DOI: 10.3390/catal10111347

Two-Step Chemoenzymatic Detection of N-Acetylneuraminic Acid−α(2-3)-Galactose Glycans

Liuqing Wen, Yuan Zheng, Kuan Jiang, Mingzhen Zhang, Shukkoor Muhammed Kondengaden, Shanshan Li, Kenneth Huang, Jing Li, Jing Song and Peng George Wang

J. Am. Chem. Soc., 2016, 138, 11473
DOI: 10.1021/jacs.6b07132

Glycoprotein In Vitro N-Glycan Processing Using Enzymes Expressed in E. coli

Libo Zhang, Yanhong Li, Riyao Li, Xiaohong Yang, Zimin Zheng, Jingxin Fu, Hai Yu and Xi Chen

Molecules, 2023, 28, 2753
DOI: 10.3390/molecules28062753

A Hitchhiker’s Guide to Problem Selection in Carbohydrate Synthesis

Xavier S. Streety, Jennifer C. Obike and Steven D. Townsend

ACS Cent. Sci., 2023, 9, 1285
DOI: 10.1021/acscentsci.3c00507

Diversity-Oriented Enzymatic Modular Assembly of ABO Histo-blood Group Antigens

Jinfeng Ye, Xian-wei Liu, Peng Peng, Wen Yi, Xi Chen, Fengshan Wang and Hongzhi Cao

ACS Catal., 2016, 6, 8140
DOI: 10.1021/acscatal.6b02755

Novel Reversible Fluorescent Glycan Linker for Functional Glycomics

Mohui Wei, Tanya R. McKitrick, Akul Y. Mehta, Chao Gao, Nan Jia, Alyssa M. McQuillan, Jamie Heimburg-Molinaro, Lijun Sun and Richard D. Cummings

Bioconjugate Chem., 2019, 30, 2897
DOI: 10.1021/acs.bioconjchem.9b00613

EASyMap-Guided Stepwise One-Pot Multienzyme (StOPMe) Synthesis and Multiplex Assays Identify Functional Tetraose-Core-Human Milk Oligosaccharides

Yuanyuan Bai, Anand Kumar Agrahari, Libo Zhang, Hai Yu, Xiaoxiao Yang, Zimin Zheng, William Su, Jingxin Fu and Xi Chen

JACS Au, 2025, 5, 822
DOI: 10.1021/jacsau.4c01094

Biosynthesis of Raffinose and Stachyose from Sucrose via an In Vitro Multienzyme System

Chaoyu Tian, Jiangang Yang, Yan Zeng, Tong Zhang, Yingbiao Zhou, Yan Men, Chun You, Yueming Zhu, Yuanxia Sun and Ning-Yi Zhou

Appl Environ Microbiol, 2019, 85
DOI: 10.1128/AEM.02306-18

Glycosyltransferase Co‐Immobilization for Natural Product Glycosylation: Cascade Biosynthesis of the C‐Glucoside Nothofagin with Efficient Reuse of Enzymes

Hui Liu, Gregor Tegl and Bernd Nidetzky

Adv Synth Catal, 2021, 363, 2157
DOI: 10.1002/adsc.202001549

Strategies for Automated Enzymatic Glycan Synthesis (AEGS)

Kai Philip Hussnaetter, Philip Palm, Andrij Pich, Matthias Franzreb, Erdmann Rapp and Lothar Elling

Biotechnology Advances, 2023, 67, 108208
DOI: 10.1016/j.biotechadv.2023.108208

The Application of Dioxygenase‐Based Chemoenzymatic Processes to the Total Synthesis of Natural Products

Ping Lan, Sebastian Ye and Martin G. Banwell

Chemistry An Asian Journal, 2019, 14, 4001
DOI: 10.1002/asia.201900988

Functional analysis of anomeric sugar kinases

Louis P. Conway and Josef Voglmeir

Carbohydrate Research, 2016, 432, 23
DOI: 10.1016/j.carres.2016.06.001

Recent advances in the enzymatic synthesis of sugar-nucleotides using nucleotidylyltransferases and glycosyltransferases

Sanaz Ahmadipour, Laura Beswick and Gavin J. Miller

Carbohydrate Research, 2018, 469, 38
DOI: 10.1016/j.carres.2018.09.002

Growing impact of sialic acid-containing glycans in future drug discovery

Priyanka Bose, Manoj K. Jaiswal, Sumit K. Singh, Rakesh K. Singh and Vinod K. Tiwari

Carbohydrate Research, 2023, 527, 108804
DOI: 10.1016/j.carres.2023.108804

Recent progress on health effects and biosynthesis of two key sialylated human milk oligosaccharides, 3′-sialyllactose and 6′-sialyllactose

Yingying Zhu, Jiameng Zhang, Wenli Zhang and Wanmeng Mu

Biotechnology Advances, 2023, 62, 108058
DOI: 10.1016/j.biotechadv.2022.108058

Glycan Assembly Strategy: From Concept to Application

Mingli Liu, Xianjin Qin and Xin‐Shan Ye

The Chemical Record, 2021, 21, 3256
DOI: 10.1002/tcr.202100183

Controllable Enzymatic Synthesis of Natural Asymmetric Human Milk Oligosaccharides

Hsien-Wei Tseng, Hsin-Kai Tseng, Kai-Eng Ooi, Cheng-En You, Hung-Kai Wang, Wen-Hua Kuo, Chi-Kung Ni, Yoshiyuki Manabe and Chun-Cheng Lin

JACS Au, 2024, 4, 4496
DOI: 10.1021/jacsau.4c00830

Chemoenzymatic Synthesis of O-Mannose Glycans Containing Sulfated or Nonsulfated HNK-1 Epitope

Tian Gao, Jingyu Yan, Chang-Cheng Liu, Angelina S. Palma, Zhimou Guo, Min Xiao, Xi Chen, Xinmiao Liang, Wengang Chai and Hongzhi Cao

J. Am. Chem. Soc., 2019, 141, 19351
DOI: 10.1021/jacs.9b08964

Recent advances on the one-pot synthesis to assemble size-controlled glycans and glycoconjugates and polysaccharides

Han Xu, Baoxing Shen, Meng Qiao, Robert J. Linhardt and Xing Zhang

Carbohydrate Polymers, 2021, 258, 117672
DOI: 10.1016/j.carbpol.2021.117672

Functional properties, structural studies and chemo-enzymatic synthesis of oligosaccharides

Chao Zhao, Yijing Wu, Xiaoyan Liu, Bin Liu, Hui Cao, Hua Yu, Satyajit D. Sarker, Lutfun Nahar and Jianbo Xiao

Trends in Food Science & Technology, 2017, 66, 135
DOI: 10.1016/j.tifs.2017.06.008

Detection Strategies for Sialic Acid and Sialoglycoconjugates

Carmanah D. Hunter and Christopher W. Cairo

ChemBioChem, 2024, 25
DOI: 10.1002/cbic.202400402

Molecular mechanism of decision-making in glycosaminoglycan biosynthesis

Douglas Sammon, Anja Krueger, Marta Busse-Wicher, Rhodri Marc Morgan, Stuart M. Haslam, Benjamin Schumann, David C. Briggs and Erhard Hohenester

Nat Commun, 2023, 14
DOI: 10.1038/s41467-023-42236-z