High-throughput dental biofilm growth analysis for multiparametric microenvironmental biochemical conditions using microfluidics

Raymond H. W. Lam, Xin Cui, Weijin Guo and Todd Thorsen

Lab Chip, 2016, 16, 1652
DOI: 10.1039/C6LC00072J

Through thick and thin: a microfluidic approach for continuous measurements of biofilm viscosity and the effect of ionic strength

F. Paquet-Mercier, M. Parvinzadeh Gashti, J. Bellavance, S. M. Taghavi and J. Greener

Lab Chip, 2016, 16, 4710
DOI: 10.1039/C6LC01101B

Thinking outside the shell: novel sensors designed from plasmon-enhanced fluorescent concentric nanoparticles

Nicolas Fontaine, Audrey Picard-Lafond, Jérémie Asselin and Denis Boudreau

Analyst, 2020, 145, 5965
DOI: 10.1039/D0AN01092H

A microfluidic approach to micromembrane synthesis for complex release profiles of nanocarriers

Nan Jia, Erica Rosella, Estelle Juère, Roxane Pouliot, Freddy Kleitz and Jesse Greener

Lab Chip, 2020, 20, 1066
DOI: 10.1039/D0LC00039F

Advances in modeling periodontal host–microbe interactions: insights from organotypic and organ-on-chip systems

Hardik Makkar and Gopu Sriram

Lab Chip, 2025, 25, 1342
DOI: 10.1039/D4LC00871E

A ratiometric nanoarchitecture for the simultaneous detection of pH and halide ions using UV plasmon-enhanced fluorescence

Jérémie Asselin, Marie-Pier Lambert, Nicolas Fontaine and Denis Boudreau

Chem. Commun., 2017, 53, 755
DOI: 10.1039/C6CC08693D

SpectIR-fluidics: completely customizable microfluidic cartridges for high sensitivity on-chip infrared spectroscopy with point-of-application studies on bacterial biofilms

Nan Jia, Arthur Daignault-Bouchard, Tianyang Deng, Thomas G. Mayerhöfer, André Bégin-Drolet and Jesse Greener

Lab Chip, 2023, 23, 3561
DOI: 10.1039/D3LC00388D

Electrochemical pH regulation in droplet microfluidics

Logan M. Wilder, Jonathan R. Thompson and Richard M. Crooks

Lab Chip, 2022, 22, 632
DOI: 10.1039/D1LC00952D

Rapid microfluidics prototyping through variotherm desktop injection molding for multiplex diagnostics

Gianmarco D. Suarez, Steevanson Bayer, Yuki Yu Kiu Tang, Domenick A. Suarez, Peter Pak-Hang Cheung and Stefan Nagl

Lab Chip, 2023, 23, 3850
DOI: 10.1039/D3LC00391D

Advancing sustainable energy solutions with microfluidic porous media

Wenhai Lei, Yuankai Yang, Shuo Yang, Ge Zhang, Jenna Poonoosamy, Anne Juel, Yves Méheust, Shervin Bagheri and Moran Wang

Lab Chip, 2025, 25, 3374
DOI: 10.1039/D5LC00206K

Microfluidic bioanalytical flow cells for biofilm studies: a review

Mohammad Pousti, Mir Pouyan Zarabadi, Mehran Abbaszadeh Amirdehi, François Paquet-Mercier and Jesse Greener

Analyst, 2019, 144, 68
DOI: 10.1039/C8AN01526K

A Systematic Review on Organ-on-a-Chip in PDMS or Hydrogel in Dentistry: An Update of the Literature

Tania Vanessa Pierfelice, Emira D’Amico, Morena Petrini, Mario Romano, Camillo D’Arcangelo, Ludovico Sbordone, Antonio Barone, Roberto Plebani and Giovanna Iezzi

Gels, 2024, 10, 102
DOI: 10.3390/gels10020102

A review of chemosensors and biosensors for monitoring biofilm dynamics

Samuel C. Saccomano, Megan P. Jewell and Kevin J. Cash

Sensors and Actuators Reports, 2021, 3, 100043
DOI: 10.1016/j.snr.2021.100043

A fluorescein sodium wide-area pH optical sensor based on amplification characteristics

Jianping Shen, Wei Wang, Siwei Zhang, Jinxin Gao, Ruidong Xia, Xin Huang, Songtao Jiang and Huiyin Wang

Optics Communications, 2021, 493, 127030
DOI: 10.1016/j.optcom.2021.127030

Development of a flow chamber system for the reproducible in vitro analysis of biofilm formation on implant materials

Henryke Rath, Sascha Nico Stumpp, Meike Stiesch and Sompop Bencharit

PLoS ONE, 2017, 12, e0172095
DOI: 10.1371/journal.pone.0172095

Multiplexed detection of respiratory virus RNA using optical pH sensors and injection-molded centrifugal microfluidics

Gianmarco Domenico Suarez, Yuki Yu Kiu Tang, Steevanson Bayer, Peter Pak-Hang Cheung and Stefan Nagl

Microchim Acta, 2025, 192
DOI: 10.1007/s00604-025-06996-3

Fluorescent pH nanosensors: Design strategies and applications

Mojtaba Shamsipur, Ali Barati and Ziba Nematifar

Journal of Photochemistry and Photobiology C: Photochemistry Reviews, 2019, 39, 76
DOI: 10.1016/j.jphotochemrev.2019.03.001

In Situ Live Monitoring of Extracellular Acidosis near Cancer Cells Using Digital Microfluidics with an Integrated Optical pH Sensor Film

Wenting Qiu, Xuyan Lin and Stefan Nagl

Anal. Chem., 2024, 96, 14456
DOI: 10.1021/acs.analchem.4c02319

Multistimuli-Responsive Camouflage Swimmers

Emil Karshalev, Rajan Kumar, Itthipon Jeerapan, Roxanne Castillo, Isaac Campos and Joseph Wang

Chem. Mater., 2018, 30, 1593
DOI: 10.1021/acs.chemmater.7b04792

Microfluidic Platform for Examination of Effect of Chewing Xylitol Gum on Salivary pH, O2, and CO2

Ivana Podunavac, Stevan Hinić, Sanja Kojić, Nina Jelenčiakova, Vasa Radonić, Bojan Petrović and Goran M. Stojanović

Applied Sciences, 2021, 11, 2049
DOI: 10.3390/app11052049

Emerging microfluidic technologies for microbiome research

Yue Yu, Hui Wen, Sihong Li, Haojie Cao, Xuefei Li, Zhixin Ma, Xiaoyi She, Lei Zhou and Shuqiang Huang

Front. Microbiol., 2022, 13
DOI: 10.3389/fmicb.2022.906979

In vitro model systems for exploring oral biofilms: From single‐species populations to complex multi‐species communities

Ting L. Luo, Michael E. Vanek, Carlos Gonzalez‐Cabezas, Carl F. Marrs, Betsy Foxman and Alexander H. Rickard

J of Applied Microbiology, 2022, 132, 855
DOI: 10.1111/jam.15200

Photoluminescent Nanoparticles for Chemical and Biological Analysis and Imaging

W. Russ Algar, Melissa Massey, Kelly Rees, Rehan Higgins, Katherine D. Krause, Ghinwa H. Darwish, William J. Peveler, Zhujun Xiao, Hsin-Yun Tsai, Rupsa Gupta, Kelsi Lix, Michael V. Tran and Hyungki Kim

Chem. Rev., 2021, 121, 9243
DOI: 10.1021/acs.chemrev.0c01176

Altered biofilm formation at plasma bonded surfaces in microchannels studied by attenuated total reflection infrared spectroscopy

Mohammad Pousti and Jesse Greener

Surface Science, 2018, 676, 56
DOI: 10.1016/j.susc.2018.01.004

Inlaid microfluidic optics: absorbance cells in clear devices applied to nitrite and phosphate detection

Edward A Luy, Sean C Morgan, Joshua J Creelman, Benjamin J Murphy and Vincent J Sieben

J. Micromech. Microeng., 2020, 30, 095001
DOI: 10.1088/1361-6439/ab9202

In-situ and continuous monitoring of pyocyanin in the formation process of Pseudomonas aeruginosa biofilms by an electrochemical biosensor chip

Lulu Liu, Xujing Cao, Wenrui Ma, Li Chen, Shunbo Li, Baoshan Hu and Yi Xu

Sensors and Actuators B: Chemical, 2021, 327, 128945
DOI: 10.1016/j.snb.2020.128945

Detection and Characterization of Bacterial Biofilms and Biofilm-Based Sensors

Riccardo Funari and Amy Q. Shen

ACS Sens., 2022, 7, 347
DOI: 10.1021/acssensors.1c02722

Flow‐Based Deacidification of Geobacter sulfurreducens Biofilms Depends on Nutrient Conditions: a Microfluidic Bioelectrochemical Study

Mir Pouyan Zarabadi, Steve J. Charette and Jesse Greener

ChemElectroChem, 2018, 5, 3645
DOI: 10.1002/celc.201800968

Sensor Micro and Nanoparticles for Microfluidic Application

Raminta Mazetyte-Stasinskiene and Johann Michael Köhler

Applied Sciences, 2020, 10, 8353
DOI: 10.3390/app10238353

Microfluidic devices for studying bacterial taxis, drug testing and biofilm formation

Sandra Pérez‐Rodríguez, José Manuel García‐Aznar and Jesús Gonzalo‐Asensio

Microbial Biotechnology, 2022, 15, 395
DOI: 10.1111/1751-7915.13775

Biofilm three-dimensional architecture influences in situ pH distribution pattern on the human enamel surface

Jin Xiao, Anderson T Hara, Dongyeop Kim, Domenick T Zero, Hyun Koo and Geelsu Hwang

Int J Oral Sci, 2017, 9, 74
DOI: 10.1038/ijos.2017.8

Advancements in Microfluidic Organ-on-a-chip for Oral Medicine

Sizheng Fan, Yongcheng Ge, Bingdao Li, Pengyuan Liu and Xiaoqiu Liu

International Dental Journal, 2025, 75, 100925
DOI: 10.1016/j.identj.2025.100925

A Microfluidic Organ-On-A-Chip: Into the Next Decade of Bone Tissue Engineering Applied in Dentistry

Muhammad Hidayat Syahruddin, Rahmi Anggraeni and Ika Dewi Ana

Future Sci. OA, 2023, 9
DOI: 10.2144/fsoa-2023-0061

Magnetotactic T-Budbots to Kill-n-Clean Biofilms

Tamanna Bhuyan, Anitha T. Simon, Surjendu Maity, Amit Kumar Singh, Siddhartha Sankar Ghosh and Dipankar Bandyopadhyay

ACS Appl. Mater. Interfaces, 2020, 12, 43352
DOI: 10.1021/acsami.0c08444

The Application of Organs-on-a-Chip in Dental, Oral, and Craniofacial Research

C. Huang, F. Sanaei, W.P.R. Verdurmen, F. Yang, W. Ji and X. F. Walboomers

J Dent Res, 2023, 102, 364
DOI: 10.1177/00220345221145555

Scanning Aperture Approach for Spatially Selective ATR-FTIR Spectroscopy: Application to Microfluidics

Maxime Joly, Tianyang Deng, Tyler A. Morhart, Garth Wells, Sven Achenbach, André Bégin-Drolet and Jesse Greener

Anal. Chem., 2021, 93, 14076
DOI: 10.1021/acs.analchem.1c01614

A video imaging method for time-dependent measurements of molecular mass transfer and biofilm dynamics in microchannels

M. Parvinzadeh Gashti, M. Zarabadi and J. Greener

MRS Advances, 2016, 1, 2099
DOI: 10.1557/adv.2016.359

Micro free‐flow isoelectric focusing with integrated optical pH sensors

Stefan Nagl

Engineering in Life Sciences, 2018, 18, 114
DOI: 10.1002/elsc.201700035

A new look at bubbles during biofilm inoculation reveals pronounced effects on growth and patterning

Farnaz Asayesh, Mir Pouyan Zarabadi and Jesse Greener

Biomicrofluidics, 2017, 11
DOI: 10.1063/1.5005932

Metal-Enhanced Hg2+-Responsive Fluorescent Nanoprobes: From Morphological Design to Application to Natural Waters

Audrey Picard-Lafond, Dominic Larivière and Denis Boudreau

ACS Omega, 2022, 7, 22944
DOI: 10.1021/acsomega.2c02985

Functionalized Mouth‐Conformable Interfaces for pH Evaluation of the Oral Cavity

Giusy Matzeu, Gili R. S. Naveh, Siddhart Agarwal, Jeffery A. Roshko, Nicholas A. Ostrovsky‐Snider, Bradley S. Napier and Fiorenzo G. Omenetto

Advanced Science, 2021, 8
DOI: 10.1002/advs.202003416

Microfluidic organ-on-chip systems for periodontal research: advances and future directions

Gopu Sriram and Hardik Makkar

Front. Bioeng. Biotechnol., 2025, 12
DOI: 10.3389/fbioe.2024.1490453

Highly pH-responsive sensor based on amplified spontaneous emission coupled to colorimetry

Qi Zhang, Jose R. Castro Smirnov, Ruidong Xia, Jose M. Pedrosa, Isabel Rodriguez, Juan Cabanillas-Gonzalez and Wei Huang

Sci Rep, 2017, 7
DOI: 10.1038/srep46265

Emerging supramolecular and living materials in oral medicine

Nicholas G. Fischer, Tsung-Yi Lin, Yuanhui Xiang, Ting Sang and Zhou Ye

Trends in Biotechnology, 2025, 43, 1876
DOI: 10.1016/j.tibtech.2025.03.006

Microfluidics for Biofilm Studies

Lu Yuan, Hervé Straub, Liubov Shishaeva and Qun Ren

Annual Rev. Anal. Chem., 2023, 16, 139
DOI: 10.1146/annurev-anchem-091522-103827

Salivary theranostics in pediatric and special care dentistry

Bojan Petrović

Balkan J Dent Med, 2019, 23, 113
DOI: 10.2478/bjdm-2019-0021

Nano-Enabled Approaches to Chemical Imaging in Biosystems

Scott T. Retterer, Jennifer L. Morrell-Falvey and Mitchel J. Doktycz

Annual Rev. Anal. Chem., 2018, 11, 351
DOI: 10.1146/annurev-anchem-061417-125635

A 3D‐Printed Customizable Platform for Multiplex Dynamic Biofilm Studies

Atul Dhall, Ravikiran Ramjee, Min Jun Oh, Kevin Tao and Geelsu Hwang

Adv Materials Technologies, 2022, 7
DOI: 10.1002/admt.202200138

Emerging Biorecognition and Transduction Schemes for Rapid Detection of Pathogenic Bacteria in Food

Diana C. Vanegas, Carmen L. Gomes, Nicholas D. Cavallaro, Daniel Giraldo‐Escobar and Eric S. McLamore

Comp Rev Food Sci Food Safe, 2017, 16, 1188
DOI: 10.1111/1541-4337.12294

Dual fluorescence ratiometric technique for micromixing characterization

David C. Bedding and Carlos H. Hidrovo

Exp Fluids, 2018, 59
DOI: 10.1007/s00348-018-2618-9

Construction of dentin-on-a-chip based on microfluidic technology and tissue engineering

Hexuan Zhang, Lingjun Li, Shujing Wang, Xiaoqiang Sun, Chunxiong Luo and Benxiang Hou

Journal of Dentistry, 2024, 146, 105028
DOI: 10.1016/j.jdent.2024.105028

A Microfluidic Platform with Nanoparticle-Based Metal-Enhanced Fluorescence for pH Mapping Acidified Aqueous Solutions by CO2 Microbubbles

Jérémie Asselin, Mazeyar Parvinzadeh Gashti, Denis Boudreau and Jesse Greener

MRS Advances, 2016, 1, 2037
DOI: 10.1557/adv.2016.288

Recent Progress in Electrochemical pH-Sensing Materials and Configurations for Biomedical Applications

M. T. Ghoneim, A. Nguyen, N. Dereje, J. Huang, G. C. Moore, P. J. Murzynowski and C. Dagdeviren

Chem. Rev., 2019, 119, 5248
DOI: 10.1021/acs.chemrev.8b00655

Biofilm interfacial acidity evaluation by pH-Responsive luminescent nanoparticle films

Padryk Merkl, Marie-Stephanie Aschtgen, Birgitta Henriques-Normark and Georgios A. Sotiriou

Biosensors and Bioelectronics, 2021, 171, 112732
DOI: 10.1016/j.bios.2020.112732

Fluorescein Derivatives as Fluorescent Probes for pH Monitoring along Recent Biological Applications

Florent Le Guern, Vanessa Mussard, Anne Gaucher, Martin Rottman and Damien Prim

IJMS, 2020, 21, 9217
DOI: 10.3390/ijms21239217

Critical shear stresses of Pseudomonas aeruginosa biofilms from dental unit waterlines studied using microfluidics and additional magnesium ions

Jesse Greener, William Y. Harvey, Cynthia Gagné-Thivierge, Sepideh Fakhari, Seyed Mohammad Taghavi, Jean Barbeau and Steve J. Charette

Physics of Fluids, 2022, 34
DOI: 10.1063/5.0076737

Hydrodynamic Effects on Biofilms at the Biointerface Using a Microfluidic Electrochemical Cell: Case Study of Pseudomonas sp.

Mir Pouyan Zarabadi, François Paquet-Mercier, Steve J. Charette and Jesse Greener

Langmuir, 2017, 33, 2041
DOI: 10.1021/acs.langmuir.6b03889