Large scale applications of immobilized enzymes call for sustainable and inexpensive solutions: rice husks as renewable alternatives to fossil-based organic resins

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RSC Adv., 2016, 6, 63256
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Engineering single-atom catalysts toward biomedical applications

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Redesigning the synthesis of vidarabine via a multienzymatic reaction catalyzed by immobilized nucleoside phosphorylases

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RSC Adv., 2015, 5, 23569
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Immobilization of horseradish peroxidase on UiO-66-NH2 for colorimetric and fluorometric sensing of nitrite

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Nanoscale, 2025, 17, 5328
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Development of simple protocols to solve the problems of enzyme coimmobilization. Application to coimmobilize a lipase and a β-galactosidase

Sara Peirce, Jose J. Virgen-Ortíz, Veymar G. Tacias-Pascacio, Nazzoly Rueda, Rocio Bartolome-Cabrero, Laura Fernandez-Lopez, Maria Elena Russo, Antonio Marzocchella and Roberto Fernandez-Lafuente

RSC Adv., 2016, 6, 61707
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Inactivation of immobilized trypsin under dissimilar conditions produces trypsin molecules with different structures

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RSC Adv., 2016, 6, 27329
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Enlarging the tools for efficient enzymatic polycondensation: structural and catalytic features of cutinase 1 from Thermobifida cellulosilytica

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Catal. Sci. Technol., 2016, 6, 3430
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The continuous oxidation of HMF to FDCA and the immobilisation and stabilisation of periplasmic aldehyde oxidase (PaoABC)

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Green Chem., 2017, 19, 4660
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Highly active, stable and self-antimicrobial enzyme catalysts prepared by biomimetic mineralization of copper hydroxysulfate

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Nanoscale, 2016, 8, 17440
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Synthesis of Fe3O4/P(St-AA) nanoparticles for enhancement of stability of the immobilized lipases

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RSC Adv., 2016, 6, 108583
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Efficient asymmetric biosynthesis of (R)-(−)-epinephrine in hydrophilic ionic liquid-containing systems

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RSC Adv., 2016, 6, 102292
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Co-immobilization of enzymes with the help of a dendronized polymer and mesoporous silica nanoparticles

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J. Mater. Chem. B, 2015, 3, 6174
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2,5-Furandicarboxaldehyde as a bio-based crosslinking agent replacing glutaraldehyde for covalent enzyme immobilization

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RSC Adv., 2022, 12, 35676
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Microfluidic immobilized enzyme reactors for continuous biocatalysis

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Temperature-responsive nanobiocatalysts with an upper critical solution temperature for high performance biotransformation and easy catalyst recycling: efficient hydrolysis of cellulose to glucose

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Green Chem., 2015, 17, 1194
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Biocatalytic ketone reductions using Biobeads for miniaturized high throughput experimentation

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Amine-functionalized magnetic nanocomposite particles for efficient immobilization of lipase: effects of functional molecule size on properties of the immobilized lipase

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RSC Adv., 2015, 5, 33313
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Modification of PEGylated enzyme with glutaraldehyde can enhance stability while avoiding intermolecular crosslinking

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Glutaraldehyde in bio-catalysts design: a useful crosslinker and a versatile tool in enzyme immobilization

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RSC Adv., 2014, 4, 1583
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Facile preparation of MOF-derived MHCo3O4&Co/C with a hierarchical porous structure for entrapping enzymes: having both high stability and catalytic activity

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Stabilization of β-Gal-3 ATCC 31382 on agarose gels: synthesis of β-(1→3) galactosides under sustainable conditions

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Pectin cross-linked enzyme aggregates (pectin-CLEAs) of glucoamylase

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Hierarchically porous magnetic Fe3O4/Fe-MOF used as an effective platform for enzyme immobilization: a kinetic and thermodynamic study of structure–activity

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The rise of continuous flow biocatalysis – fundamentals, very recent developments and future perspectives

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Sustainable synthesis of N-acetyllactosamine using an immobilized β-galactosidase on a tailor made porous polymer

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Open-mouthed hybrid microcapsules with elevated enzyme loading and enhanced catalytic activity

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Characterization of lipase fromCandida rugosaentrapped in alginate beads to enhance its thermal stability and recyclability

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Immobilisation and stabilisation of glycosylated enzymes on boronic acid-functionalised silica nanoparticles

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Cellulose as an efficient matrix for lipase and transaminase immobilization

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Preparation of a magnetic responsive immobilized lipase–cellulose microgel catalyst system: role of the surface properties of the magnetic cellulose microgel

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RSC Adv., 2016, 6, 7339
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Immobilized enzymes in inorganic hybrid nanoflowers for biocatalytic and biosensing applications

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Marine chitin upcycling with immobilized chitinolytic enzymes: current state and prospects

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Green Chem., 2023, 25, 467
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Enzyme immobilization on silicate glass through simple adsorption of dendronized polymer–enzyme conjugates for localized enzymatic cascade reactions

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Synthesis of papain–polyacrylamide hydrogel microspheres and their catalytic application

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Lipase immobilization towards improved productivity on kinetic resolutions by a continuous-flow process

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RSC Adv., 2015, 5, 102409
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Nanocapsule engineering endows aldolase with robust organic-solvent tolerance and enhanced catalytic efficiency

Can Cui, Xuerui Jin and Yunzi Luo

Catal. Sci. Technol., 2026, 16, 2481
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Towards feasible and scalable solvent-free enzymatic polycondensations: integrating robust biocatalysts with thin film reactions

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Green Chem., 2015, 17, 1756
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Facile fabrication of a recyclable nanobiocatalyst: immobilization of Burkholderia cepacia lipase on carbon nanofibers for the kinetic resolution of a racemic atenolol intermediate

Surbhi Soni, Bharat Prasad Dwivedee and Uttam Chand Banerjee

RSC Adv., 2018, 8, 27763
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Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization

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Chem. Soc. Rev., 2022, 51, 6251
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Recent developments in microreactor technology for biocatalysis applications

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Amine dehydrogenases: efficient biocatalysts for the reductive amination of carbonyl compounds

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Green Chem., 2017, 19, 453
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Increase of stability of oleate hydratase by appropriate immobilization technique and conditions

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Journal of Molecular Catalysis B: Enzymatic, 2015, 119, 40
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Functionalized Magnetic Bacterial Cellulose Beads as Carrier for Lecitase® Ultra Immobilization

Radosław Drozd, Magdalena Szymańska, Rafał Rakoczy, Adam Junka, Patrycja Szymczyk and Karol Fijałkowski

Appl Biochem Biotechnol, 2019, 187, 176
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l-Amino Acid Production by a Immobilized Double-Racemase Hydantoinase Process: Improvement and Comparison with a Free Protein System

María Rodríguez-Alonso, Felipe Rodríguez-Vico, Francisco Las Heras-Vázquez and Josefa Clemente-Jiménez

Catalysts, 2017, 7, 192
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Desorption of Lipases Immobilized on Octyl-Agarose Beads and Coated with Ionic Polymers after Thermal Inactivation. Stronger Adsorption of Polymers/Unfolded Protein Composites

Jose Virgen-Ortíz, Sara Pedrero, Laura Fernandez-Lopez, Nerea Lopez-Carrobles, Beatriz Gorines, Cristina Otero and Roberto Fernandez-Lafuente

Molecules, 2017, 22, 91
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Efficient Biosynthesis of Phosphatidylserine in a Biphasic System through Parameter Optimization

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Processes, 2023, 11, 2368
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An Enzyme Net Coating the Surface of Nanoparticles: A Simple and Efficient Method for the Immobilization of Phospholipase D

Binglin Li, Jiao Wang, Xiaoli Zhang and Binxia Zhao

Ind. Eng. Chem. Res., 2016, 55, 10555
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Immobilization of γ‐Glutamyl Transpeptidase from Equine Kidney for the Synthesis of kokumi Compounds

Margherita Bruni, Marina S. Robescu, Daniela Ubiali, Giorgio Marrubini, Renzo Vanna, Carlo Morasso, Ilaria Benucci, Giovanna Speranza and Teodora Bavaro

ChemCatChem, 2020, 12, 210
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Study of poly (butylene adipate-co-terephthalate)/beta-cyclodextrin/polyvinyl alcohol, doped Mg2+, coated recycled Fe3O4 particle carriers for enhancing immobilization penicillin G acylase performance

Monier Alhadi Abdelrahman Mohammed, Zhenbin Chen, Xueyan Du, Bin Li, Jinhan Zhang and Tai Kang

Colloid Polym Sci, 2025, 303, 937
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Nature Inspired Solutions for Polymers: Will Cutinase Enzymes Make Polyesters and Polyamides Greener?

Valerio Ferrario, Alessandro Pellis, Marco Cespugli, Georg Guebitz and Lucia Gardossi

Catalysts, 2016, 6, 205
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Immobilization of horseradish peroxidase on hierarchically porous magnetic metal-organic frameworks for visual detection and efficient degradation of 2,4-dichlorophenol in simulated wastewater

Xia Gao, Huibin Pan, Ke Yang, Jiamei Dang, Chengfang Qiao, Yongliang Liu and Chunsheng Zhou

Biochemical Engineering Journal, 2023, 190, 108760
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On the relationship between structure and catalytic effectiveness in solid surface-immobilized enzymes: Advances in methodology and the quest for a single-molecule perspective

Juan M. Bolivar and Bernd Nidetzky

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2020, 1868, 140333
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Functionalization of Porous Cellulose with Glyoxyl Groups as a Carrier for Enzyme Immobilization and Stabilization

Sandro Martins de Oliveira, Susana Velasco-Lozano, Alejandro H. Orrego, Javier Rocha-Martín, Sonia Moreno-Pérez, José M. Fraile, Fernando López-Gallego and Jose Manuel Guisán

Biomacromolecules, 2021, 22, 927
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Recycling of hyoscyamine 6β-hydroxylase for the in vitro production of anisodamine and scopolamine

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Single‐Particle Studies to Advance the Characterization of Heterogeneous Biocatalysts

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ChemCatChem, 2018, 10, 654
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Chemical Modification in the Design of Immobilized Enzyme Biocatalysts: Drawbacks and Opportunities

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The Chemical Record, 2016, 16, 1436
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Modelling of methylene blue adsorption using peroxidase immobilized functionalized Buckypaper/polyvinyl alcohol membrane via ant colony optimization

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Environmental Pollution, 2020, 259, 113940
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Multicatalytic Hybrid Materials for Biocatalytic and Chemoenzymatic Cascades—Strategies for Multicatalyst (Enzyme) Co-Immobilization

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Catalysts, 2021, 11, 936
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Chemical amination of lipases improves their immobilization on octyl-glyoxyl agarose beads

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Catalysis Today, 2016, 259, 107
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Optimization of the coating of octyl-CALB with ionic polymers to improve stability and decrease enzyme leakage

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Catalysts, 2020, 10, 697
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Nanozymes-engineered metal–organic frameworks for enhanced microwave thermodynamic therapy in PDX of hepatic carcinoma

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Chemical Engineering Journal, 2022, 450, 138092
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A Comprehensive Guide to Enzyme Immobilization: All You Need to Know

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Molecules, 2025, 30, 939
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Region-Directed Enzyme Immobilization through Engineering Protein Surface with Histidine Clusters

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ACS Appl. Mater. Interfaces, 2024, 16, 833
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Evaluation of different lipase biocatalysts in the production of biodiesel from used cooking oil: Critical role of the immobilization support

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Fuel, 2017, 200, 1
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Nitrile hydratase mediated green synthesis of lactamide by immobilizing Rhodococcus pyridinivorans NIT-36 cells on N, N′-Methylene bis-acrylamide activated chitosan

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International Journal of Biological Macromolecules, 2020, 161, 168
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Challenges and perspectives of the β-galactosidase enzyme

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Preparative Isolation of N‐Glycans from Natural Sources Mediated by a Deglycosylating Heterogeneous Biocatalyst in Flow

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ChemSusChem, 2025, 18
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Electrospun nanofibrous membranes containing epoxy groups and hydrophilic polyethylene oxide chain for highly active and stable covalent immobilization of lipase

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From Protein Features to Sensing Surfaces

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Optimal spacer arm microenvironment for the immobilization of recombinant Protein A on heterofunctional amino-epoxy agarose supports

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Use of Immobilized Amine Transaminase from Vibrio fluvialis under Flow Conditions for the Synthesis of (S)‐1‐(5‐Fluoropyrimidin‐2‐yl)‐ethanamine

Riccardo Semproli, Gianmarco Vaccaro, Erica E. Ferrandi, Marta Vanoni, Teodora Bavaro, Giorgio Marrubini, Francesca Annunziata, Paola Conti, Giovanna Speranza, Daniela Monti, Lucia Tamborini and Daniela Ubiali

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Improvement of Trehalose Production by Immobilized Trehalose Synthase from Thermus thermophilus HB27

Jing Sun, Shizeng Wang, Wenna Li, Ruimin Li, Sheng Chen, Hyon Il Ri, Tae Mun Kim, Myong Su Kang, Lu Sun, Xinxiao Sun and Qipeng Yuan

Molecules, 2018, 23, 1087
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Incorporation and influence of natural gums in an alginate matrix for Serratia plymuthica immobilization and isomaltulose production

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Construction of Novel Enzyme–Graphene Oxide Catalytic Interface with Improved Enzymatic Performance and Its Assembly Mechanism

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Sol–gel immobilization of haloalkane dehalogenase from Bradyrhizobium japonicum for the remediation 1,2-dibromoethane

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Immobilization of endoglucanase on kaolin by adsorption and covalent bonding

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Advances in cofactor immobilization for enhanced continuous-flow biocatalysis

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Parallel Interconnected Kinetic Asymmetric Transformation (PIKAT) with an Immobilized ω-Transaminase in Neat Organic Solvent

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Reversible Supramolecular Surface Attachment of Enzyme–Polymer Conjugates for the Design of Biocatalytic Filtration Membranes

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Enzymatic degradation of ginkgolic acid by laccase immobilized on novel electrospun nanofiber mat

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Biobased, Internally pH-Sensitive Materials: Immobilized Yellow Fluorescent Protein as an Optical Sensor for Spatiotemporal Mapping of pH Inside Porous Matrices

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An ideal enzyme immobilization carrier: a hierarchically porous cellulose monolith fabricated by phase separation method

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Encapsulated NOLA™ Fit 5500 Lactase—An Economically Beneficial Way to Obtain Lactose-Free Milk at Low Temperature

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An Insight into Enzymatic Immobilization Techniques on the Saccharification of Lignocellulosic Biomass

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A General Overview of Support Materials for Enzyme Immobilization: Characteristics, Properties, Practical Utility

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Carrier-Free Immobilization of Lipase from Candida rugosa with Polyethyleneimines by Carboxyl-Activated Cross-Linking

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Immobilized Dehydrogenases for the Biosynthesis of Phenylacetic Acids

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Stimuli‐Responsive Manganese Single‐Atom Nanozyme for Tumor Therapy via Integrated Cascade Reactions

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Comparative Study of Immobilized Biolipasa‐R for Second Generation Biodiesel Production from an Acid Oil

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Immobilization of Aspergillus oryzae β-galactosidase in cation functionalized agarose matrix and its application in the synthesis of lactulose

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Immobilization of a multi‐enzyme system for L‐amino acids production

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Interfacial Polymerization of Dopamine in a Pickering Emulsion: Synthesis of Cross-Linkable Colloidosomes and Enzyme Immobilization at Oil/Water Interfaces

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Immobilization/Stabilization of Ficin Extract on Glutaraldehyde-Activated Agarose Beads. Variables That Control the Final Stability and Activity in Protein Hydrolyses

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Understanding Potentials and Restrictions of Solvent‐Free Enzymatic Polycondensation of Itaconic Acid: An Experimental and Computational Analysis

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Developing a Novel Enzyme Immobilization Process by Activation of Epoxy Carriers with Glucosamine for Pharmaceutical and Food Applications

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Self Assembly through Sonication: An Expeditious and Green Approach for the Synthesis of Organic‐Inorganic Hybrid Nanopetals and their Application as Biocatalyst

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Reticular framework materials-enhanced biomimetic cascade catalysis systems for boosting advanced biosensing

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Library of Norcoclaurine Synthases and Their Immobilization for Biocatalytic Transformations

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Challenges and Opportunities: Porous Supports in Carbonic Anhydrase Immobilization

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Tosylated cloisite as a new heterofunctional carrier for covalent immobilization of lipase and its utilization for production of biodiesel from waste frying oil

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Novel polymeric supports for lipase immobilization and their application in the transesterification of soybean oil for biodiesel production

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Facile preparation of Fe3O4@MOF core-shell microspheres for lipase immobilization

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Asymmetric Reduction of Prochiral Ketones by Using Self‐Sufficient Heterogeneous Biocatalysts Based on NADPH‐Dependent Ketoreductases

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Single‐Particle and Single‐Molecule Characterization of Immobilized Enzymes: A Multiscale Path toward Optimizing Heterogeneous Biocatalysts

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Enzyme Mimics for Engineered Biomimetic Cascade Nanoreactors: Mechanism, Applications, and Prospects

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Immobilization of α-Amylase from Anoxybacillus sp. SK3-4 on ReliZyme and Immobead Supports

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Molecules, 2016, 21, 1196
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Sequential optimization strategy for the immobilization of Erwinia sp. D12 cells and the production of isomaltulose with high stability and prebiotic potential

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Synergistic Enhancement of Enzyme Performance and Resilience via Orthogonal Peptide–Protein Chemistry Enabled Multilayer Construction

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Immobilization of Neutral Protease from Bacillus subtilis for Regioselective Hydrolysis of Acetylated Nucleosides: Application to Capecitabine Synthesis

Teodora Bavaro, Giulia Cattaneo, Immacolata Serra, Ilaria Benucci, Massimo Pregnolato and Marco Terreni

Molecules, 2016, 21, 1621
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Encapsulation and biochemical characterization of Lipase on a silica-coated@ZIF-8@/graphene oxide (GSLZIF) and its application on the biodiesel production

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Immobilization of Arabidopsis thaliana Hydroxynitrile Lyase (AtHNL) on EziG Opal

José Coloma, Tim Lugtenburg, Muhammad Afendi, Mattia Lazzarotto, Paula Bracco, Peter-Leon Hagedoorn, Lucia Gardossi and Ulf Hanefeld

Catalysts, 2020, 10, 899
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Sequential application of waste whey as a medium component for Kluyveromyces lactis cultivation and a co-feeder for lipase immobilization by CLEA method

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Appl Microbiol Biotechnol, 2017, 101, 3617
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