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Site-selective incorporation and ligation of protein aldehydes

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Designing logical codon reassignment – Expanding the chemistry in biology

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Expanding the chemical diversity of lasso peptide MccJ25 with genetically encoded noncanonical amino acids

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Chem. Commun., 2015, 51, 409
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A genetically encoded aldehyde for rapid protein labelling

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Chem. Commun., 2014, 50, 7424
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Genetically encoded fluorophenylalanines enable insights into the recognition of lysine trimethylation by an epigenetic reader

Yan-Jiun Lee, M. J. Schmidt, Jeffery M. Tharp, Annemarie Weber, Amber L. Koenig, Hong Zheng, Jianmin Gao, Marcey L. Waters, Daniel Summerer and Wenshe R. Liu

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Side-chain-to-tail cyclization of ribosomally derived peptides promoted by aryl and alkyl amino-functionalized unnatural amino acids

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Org. Biomol. Chem., 2016, 14, 5803
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Engineering of enzymes using non-natural amino acids

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Construction of Aryl Azide‐ and Triazole‐Based Unnatural Amino Acid Motifs via the Pd(II)‐Catalyzed C(sp3)−H Arylation

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Azidoethoxyphenylalanine as a Vibrational Reporter and Click Chemistry Partner in Proteins

Elise M. Tookmanian, Christine M. Phillips‐Piro, Edward E. Fenlon and Scott H. Brewer

Chemistry A European J, 2015, 21, 19096
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Engineering Pyrrolysine Systems for Genetic Code Expansion and Reprogramming

Daniel L. Dunkelmann and Jason W. Chin

Chem. Rev., 2024, 124, 11008
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Probing the Catalytic Charge-Relay System in Alanine Racemase with Genetically Encoded Histidine Mimetics

Vangmayee Sharma, Yane-Shih Wang and Wenshe R. Liu

ACS Chem. Biol., 2016, 11, 3305
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Efficient Reassignment of a Frequent Serine Codon in Wild-Type Escherichia coli

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ACS Synth. Biol., 2016, 5, 163
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Design, synthesis and biochemical evaluation of novel 2-amino-3-(7-methoxybenzo[d][1,3]dioxol-5-yl)propanoic acid using Horseradish peroxidase (HRP) activity, cellular ROS inhibition and molecular docking study

Koffi Sénam Etsè, Albert Demonceau, Guillermo Zaragoza, Didier Serteyn and Ange Mouithys-Mickalad

Journal of Molecular Structure, 2022, 1250, 131668
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Machine Learning Enables Prediction of Pyrrolysyl-tRNA Synthetase Substrate Specificity

Qunfeng Zhang, Wenlong Zheng, Zhongdi Song, Qiang Zhang, Lirong Yang, Jianping Wu, Jianping Lin, Gang Xu and Haoran Yu

ACS Synth. Biol., 2023, 12, 2403
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An evolved pyrrolysyl-tRNA synthetase with polysubstrate specificity expands the toolbox for engineering enzymes with incorporation of noncanonical amino acids

Ke Liu, Ling Jiang, Shuang Ma, Zhongdi Song, Lun Wang, Qunfeng Zhang, Renhao Xu, Lirong Yang, Jianping Wu and Haoran Yu

Bioresour. Bioprocess., 2023, 10
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Genetically expanded cell‐free protein synthesis using endogenous pyrrolysyl orthogonal translation system

Yonatan Chemla, Eden Ozer, Orr Schlesinger, Vincent Noireaux and Lital Alfonta

Biotech & Bioengineering, 2015, 112, 1663
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Genetic Code Expansion History and Modern Innovations

Alan Costello, Alexander A. Peterson, Pei-Hsin Chen, Rustam Bagirzadeh, David L. Lanster and Ahmed H. Badran

Chem. Rev., 2024, 124, 11962
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Noncanonical amino acids as doubly bio-orthogonal handles for one-pot preparation of protein multiconjugates

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Pyrrolysine Amber Stop‐Codon Suppression: Development and Applications

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ChemBioChem, 2017, 18, 1973
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Electron‐deficient p‐benzoyl‐l‐phenylalanine derivatives increase covalent chemical capture yields for protein–protein interactions

Cassandra M. Joiner, Meghan E. Breen and Anna K. Mapp

Protein Science, 2019, 28, 1163
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Ribosomal Incorporation of Thioxanthone as a Noncanonical Amino Acid Facilitates the Engineering of Photoenzymes

Marco Seifert, Martin Termathe, Luca Nardo and Matthias Höhne

ChemCatChem, 2025, 17
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Genetic Code Expansion: Recent Developments and Emerging Applications

Yujia Huang, Pan Zhang, Haoyu Wang, Yan Chen, Tao Liu and Xiaozhou Luo

Chem. Rev., 2025, 125, 523
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Sirtuin‐Derived Covalent Binder for the Selective Recognition of Protein Crotonylation

Yanli Ji, Lin Sun, Yao Chen, Hongqiang Qin and Weimin Xuan

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Double Nitroxide Labeling by Copper-Catalyzed Azide–Alkyne Cycloadditions with Noncanonical Amino Acids for Electron Paramagnetic Resonance Spectroscopy

Pia Widder, Frederic Berner, Daniel Summerer and Malte Drescher

ACS Chem. Biol., 2019, 14, 839
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Two Rapid Catalyst-Free Click Reactions for In Vivo Protein Labeling of Genetically Encoded Strained Alkene/Alkyne Functionalities

Yadagiri Kurra, Keturah A. Odoi, Yan-Jiun Lee, Yanyan Yang, Tongxiang Lu, Steven E. Wheeler, Jessica Torres-Kolbus, Alexander Deiters and Wenshe R. Liu

Bioconjugate Chem., 2014, 25, 1730
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A Designed, Highly Efficient Pyrrolysyl-tRNA Synthetase Mutant Binds o-Chlorophenylalanine Using Two Halogen Bonds

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Journal of Molecular Biology, 2022, 434, 167534
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Sirtuin‐Derived Covalent Binder for the Selective Recognition of Protein Crotonylation

Yanli Ji, Lin Sun, Yao Chen, Hongqiang Qin and Weimin Xuan

Angew Chem Int Ed, 2022, 61
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Pyrrolysyl-tRNA synthetase: An ordinary enzyme but an outstanding genetic code expansion tool

Wei Wan, Jeffery M. Tharp and Wenshe R. Liu

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2014, 1844, 1059
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Discovery and Optimization of Chromeno[2,3-c]pyrrol-9(2H)-ones as Novel Selective and Orally Bioavailable Phosphodiesterase 5 Inhibitors for the Treatment of Pulmonary Arterial Hypertension

Deyan Wu, Tianhua Zhang, Yiping Chen, Yadan Huang, Haiju Geng, Yanfa Yu, Chen Zhang, Zengwei Lai, Yinuo Wu, Xiaolei Guo, Jianwen Chen and Hai-Bin Luo

J. Med. Chem., 2017, 60, 6622
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