Advances in microfluidics-based experimental methods for neuroscience research

Jae Woo Park, Hyung Joon Kim, Myeong Woo Kang and Noo Li Jeon

Lab Chip, 2013, 13, 509
DOI: 10.1039/c2lc41081h

Manipulating biological agents and cells in micro-scale volumes for applications in medicine

Savas Tasoglu, Umut Atakan Gurkan, ShuQi Wang and Utkan Demirci

Chem. Soc. Rev., 2013, 42, 5788
DOI: 10.1039/c3cs60042d

One-photon and two-photon stimulation of neurons in a microfluidic culture system

Jae Myung Jang, Jeonghyeon Lee, Hyeongeun Kim, Noo Li Jeon and Woonggyu Jung

Lab Chip, 2016, 16, 1684
DOI: 10.1039/C6LC00065G

Microfluidic devices as tools for mimicking the in vivo environment

Karina Ziółkowska, Radosław Kwapiszewski and Zbigniew Brzózka

New J. Chem., 2011, 35, 979
DOI: 10.1039/c0nj00709a

Optimizing cell encapsulation condition in ECM-Collagen I hydrogels to support 3D neuronal cultures

Doris Lam, Heather A. Enright, Sandra K.G. Peters, Monica L. Moya, David A. Soscia, Jose Cadena, Javier A. Alvarado, Kristen S. Kulp, Elizabeth K. Wheeler and Nicholas O. Fischer

Journal of Neuroscience Methods, 2020, 329, 108460
DOI: 10.1016/j.jneumeth.2019.108460

MatriGrid® Based Biological Morphologies: Tools for 3D Cell Culturing

Patrick Mai, Jörg Hampl, Martin Baca, Dana Brauer, Sukhdeep Singh, Frank Weise, Justyna Borowiec, André Schmidt, Johanna Merle Küstner, Maren Klett, Michael Gebinoga, Insa S. Schroeder, Udo R. Markert, Felix Glahn, Berit Schumann, Diana Eckstein and Andreas Schober

Bioengineering, 2022, 9, 220
DOI: 10.3390/bioengineering9050220

Critical Considerations for the Design of Multi-Organ Microphysiological Systems (MPS)

Mridu Malik, Yang Yang, Parinaz Fathi, Gretchen J. Mahler and Mandy B. Esch

Front. Cell Dev. Biol., 2021, 9
DOI: 10.3389/fcell.2021.721338

Microphysiological Human Brain and Neural Systems-on-a-Chip: Potential Alternatives to Small Animal Models and Emerging Platforms for Drug Discovery and Personalized Medicine

Alexander P. Haring, Harald Sontheimer and Blake N. Johnson

Stem Cell Rev and Rep, 2017, 13, 381
DOI: 10.1007/s12015-017-9738-0

A Device for Long-Term Perfusion, Imaging, and Electrical Interfacing of Brain Tissue In vitro

Nathaniel J. Killian, Varadraj N. Vernekar, Steve M. Potter and Jelena Vukasinovic

Front. Neurosci., 2016, 10
DOI: 10.3389/fnins.2016.00135

Multi‐Sensor Origami Platform: A Customizable System for Obtaining Spatiotemporally Precise Functional Readouts in 3D Models

Noam Rahav, Denise Marrero, Adi Soffer, Emma Glickman, Megane Beldjilali‐Labro, Yakey Yaffe, Keshet Tadmor, Yael Leichtmann‐Bardoogo, Uri Ashery and Ben M. Maoz

Advanced Science, 2024, 11
DOI: 10.1002/advs.202305555

Probing function in 3D neuronal cultures: A survey of 3D multielectrode array advances

Doris Lam, Nicholas O. Fischer and Heather A. Enright

Current Opinion in Pharmacology, 2021, 60, 255
DOI: 10.1016/j.coph.2021.08.003

A Novel 3D Helical Microelectrode Array for In Vitro Extracellular Action Potential Recording

Negar Geramifard, Jennifer Lawson, Stuart F. Cogan and Bryan James Black

Micromachines, 2022, 13, 1692
DOI: 10.3390/mi13101692

Microdevice Platform for In Vitro Nervous System and Its Disease Model

Jin-Ha Choi, Hyeon-Yeol Cho and Jeong-Woo Choi

Bioengineering, 2017, 4, 77
DOI: 10.3390/bioengineering4030077

Neuron(s)-on-a-Chip: A Review of the Design and Use of Microfluidic Systems for Neural Tissue Culture

David Choy Buentello, Mariana García-Corral, Grissel Trujillo-de Santiago and Mario Moisés Alvarez

IEEE Rev. Biomed. Eng., 2024, 17, 243
DOI: 10.1109/RBME.2022.3217486

Multilayer microfluidic systems with indium-tin-oxide microelectrodes for studying biological cells

Hsiang-Chiu Wu, Jia-Bo Lyau, Min-Hsuan Lin, Yung-Jen Chuang and Hsin Chen

J. Micromech. Microeng., 2017, 27, 075013
DOI: 10.1088/1361-6439/aa71d6

From Cellular Cultures to Cellular Spheroids: Is Impedance Spectroscopy a Viable Tool for Monitoring Multicellular Spheroid (MCS) Drug Models?

Frank A. Alexander, Dorielle Tucker Price and Shekhar Bhansali

IEEE Rev. Biomed. Eng., 2013, 6, 63
DOI: 10.1109/RBME.2012.2222023

Emerging neurotechnology for antinoceptive mechanisms and therapeutics discovery

Bryan J. Black, Rahul Atmaramani, Sarah Plagens, Zachary T. Campbell, Gregory Dussor, Theodore J. Price and Joseph J. Pancrazio

Biosensors and Bioelectronics, 2019, 126, 679
DOI: 10.1016/j.bios.2018.11.015

Microelectrothermoforming (μETF): one-step versatile 3D shaping of flexible microelectronics for enhanced neural interfaces

Dong Hyeon Lee, Younghoon Park, Yoon Seo, Hannah Noh, Hyunbeen Jeong, Jongmo Seo, Min-Ho Seo, Kyungsik Eom and Joonsoo Jeong

npj Flex Electron, 2025, 9
DOI: 10.1038/s41528-024-00378-0

Hybrid multiphoton volumetric functional imaging of large-scale bioengineered neuronal networks

Hod Dana, Anat Marom, Shir Paluch, Roman Dvorkin, Inbar Brosh and Shy Shoham

Nat Commun, 2014, 5
DOI: 10.1038/ncomms4997

Spontaneous Activity Characteristics of 3D “Optonets”

Anat Marom, Erez Shor, Shulamit Levenberg and Shy Shoham

Front. Neurosci., 2017, 10
DOI: 10.3389/fnins.2016.00602

Experimental and Computational Methods for the Study of Cerebral Organoids: A Review

Daniele Poli, Chiara Magliaro and Arti Ahluwalia

Front. Neurosci., 2019, 13
DOI: 10.3389/fnins.2019.00162

Designing Neural Networks in Culture

B.C. Wheeler and G.J. Brewer

Proc. IEEE, 2010, 98, 398
DOI: 10.1109/JPROC.2009.2039029

3-D multi-electrode arrays detect early spontaneous electrophysiological activity in 3-D neuronal-astrocytic co-cultures

Varadraj N. Vernekar and Michelle C. LaPlaca

Biomed. Eng. Lett., 2020, 10, 579
DOI: 10.1007/s13534-020-00166-5

Chronic network stimulation enhances evoked action potentials

A N Ide, A Andruska, M Boehler, B C Wheeler and G J Brewer

J. Neural Eng., 2010, 7, 016008
DOI: 10.1088/1741-2560/7/1/016008

Autonomous beating rate adaptation in human stem cell-derived cardiomyocytes

George Eng, Benjamin W. Lee, Lev Protas, Mark Gagliardi, Kristy Brown, Robert S. Kass, Gordon Keller, Richard B. Robinson and Gordana Vunjak-Novakovic

Nat Commun, 2016, 7
DOI: 10.1038/ncomms10312

The Need for Physiological Micro-Nanofluidic Systems of the Brain

Jean-Philippe Frimat and Regina Luttge

Front. Bioeng. Biotechnol., 2019, 7
DOI: 10.3389/fbioe.2019.00100

Microfabricated porous SU-8 membranes as innervation interfaces for hiPSC-neurons in microfluidic devices

T Salpavaara, T Joki, A Skogberg, M T Calejo, J Lekkala, S Narkilahti and P Kallio

J. Phys. Commun., 2021, 5, 115003
DOI: 10.1088/2399-6528/ac314d

A three-dimensional micro-electrode array for in-vitro neuronal interfacing

Andrea Spanu, Nicolò Colistra, Pasqualina Farisello, Alexander Friz, Noel Arellano, Charles T Rettner, Annalisa Bonfiglio, Luisa Bozano and Sergio Martinoia

J. Neural Eng., 2020, 17, 036033
DOI: 10.1088/1741-2552/ab9844

Microfluidic 3D Cell Culture: Potential Application For Tissue-Based Bioassays

XiuJun (James) Li, Alejandra V Valadez, Peng Zuo and Zhihong Nie

Bioanalysis, 2012, 4, 1509
DOI: 10.4155/bio.12.133

Electrical monitoring approaches in 3-dimensional cell culture systems: Toward label-free, high spatiotemporal resolution, and high-content data collection in vitro

Yagmur Demircan Yalcin and Regina Luttge

Organs-on-a-Chip, 2021, 3, 100006
DOI: 10.1016/j.ooc.2021.100006

Recent advances in three-dimensional microelectrode array technologies for in vitro and in vivo cardiac and neuronal interfaces

Jong Seob Choi, Heon Joon Lee, Swaminathan Rajaraman and Deok-Ho Kim

Biosensors and Bioelectronics, 2021, 171, 112687
DOI: 10.1016/j.bios.2020.112687

Therapeutic Translation of iPSCs for Treating Neurological Disease

Diana X. Yu, Maria C. Marchetto and Fred H. Gage

Cell Stem Cell, 2013, 12, 678
DOI: 10.1016/j.stem.2013.05.018

360° size-adjustable microelectrode array system for electrophysiological monitoring of cerebral organoids

Takashi Ozaki, Norikazu Ohta, Jiaju Ma and Minoru Hirano

Front. Bioeng. Biotechnol., 2025, 13
DOI: 10.3389/fbioe.2025.1596009

Advancing a MEMS-Based 3D Cell Culture System for in vitro Neuro-Electrophysiological Recordings

Alex J. Bastiaens, Jean-Philippe Frimat, Teun van Nunen, Bart Schurink, Erik F. G. A. Homburg and Regina Luttge

Front. Mech. Eng., 2018, 4
DOI: 10.3389/fmech.2018.00021

Microfluidic Brain-on-a-Chip: Perspectives for Mimicking Neural System Disorders

Mirza Ali Mofazzal Jahromi, Amir Abdoli, Mohammad Rahmanian, Hassan Bardania, Mehrdad Bayandori, Seyed Masoud Moosavi Basri, Alireza Kalbasi, Amir Reza Aref, Mahdi Karimi and Michael R Hamblin

Mol Neurobiol, 2019, 56, 8489
DOI: 10.1007/s12035-019-01653-2

Instrumented Microphysiological Systems for Real-Time Measurement and Manipulation of Cellular Electrochemical Processes

Jonathan R. Soucy, Adam J. Bindas, Abigail N. Koppes and Ryan A. Koppes

iScience, 2019, 21, 521
DOI: 10.1016/j.isci.2019.10.052

Design and fabrication of a scalable liver-lobule-on-a-chip microphysiological platform

Amin A Banaeiyan, Jannick Theobald, Jurgita Paukštyte, Stefan Wölfl, Caroline B Adiels and Mattias Goksör

Biofabrication, 2017, 9, 015014
DOI: 10.1088/1758-5090/9/1/015014

Development of neurons on micropatterns reveals that growth cone responds to a sharp change of concentration of laminin

Shige Xing, Wenwen Liu, Zhuo Huang, Long Chen, Kang Sun, Dong Han, Wei Zhang and Xingyu Jiang

Electrophoresis, 2010, 31, 3144
DOI: 10.1002/elps.201000133

Using Human‐Induced Pluripotent Stem Cell Derived Neurons on Microelectrode Arrays to Model Neurological Disease: A Review

Shiya Lv, Enhui He, Jinping Luo, Yaoyao Liu, Wei Liang, Shihong Xu, Kui Zhang, Yan Yang, Mixia Wang, Yilin Song, Yirong Wu and Xinxia Cai

Advanced Science, 2023, 10
DOI: 10.1002/advs.202301828

A mesh microelectrode array for non-invasive electrophysiology within neural organoids

Matthew McDonald, David Sebinger, Lisa Brauns, Laura Gonzalez-Cano, Yotam Menuchin-Lasowski, Michael Mierzejewski, Olympia-Ekaterini Psathaki, Angelika Stumpf, Jenny Wickham, Thomas Rauen, Hans Schöler and Peter D. Jones

Biosensors and Bioelectronics, 2023, 228, 115223
DOI: 10.1016/j.bios.2023.115223

Stem Cells in Neurotoxicology/Developmental Neurotoxicology: Current Scenario and Future Prospects

S. Singh, A. Srivastava, V. Kumar, A. Pandey, D. Kumar, C. S. Rajpurohit, V. K. Khanna, S. Yadav and A. B. Pant

Mol Neurobiol, 2016, 53, 6938
DOI: 10.1007/s12035-015-9615-2

Ferromagnetic Micropallets for Magnetic Capture of Single Adherent Cells

Nicholas M. Gunn, Ruth Chang, Trisha Westerhof, Guann-Pyng Li, Mark Bachman and Edward L. Nelson

Langmuir, 2010, 26, 17703
DOI: 10.1021/la101960v

Simple Precision Creation of Digitally Specified, Spatially Heterogeneous, Engineered Tissue Architectures

Umut Atakan Gurkan, Yantao Fan, Feng Xu, Burcu Erkmen, Emel Sokullu Urkac, Gunes Parlakgul, Jacob Bernstein, Wangli Xing, Edward S. Boyden and Utkan Demirci

Advanced Materials, 2013, 25, 1192
DOI: 10.1002/adma.201203261

Central Nervous System and its Disease Models on a Chip

YoonYoung Yi, JiSoo Park, Jaeho Lim, C. Justin Lee and Sang-Hoon Lee

Trends in Biotechnology, 2015, 33, 762
DOI: 10.1016/j.tibtech.2015.09.007

Material considerations forin vitroneural interface technology

Yoonkey Nam

MRS Bull., 2012, 37, 566
DOI: 10.1557/mrs.2012.98

Bundling of axons through a capillary alginate gel enhances the detection of axonal action potentials using microelectrode arrays

Dale S. George, Wesley A. Anderson, Frank Sommerhage, Alicia R. Willenberg, Robert B. Hines, Alexander J. Bosak, Bradley J. Willenberg and Stephen Lambert

J Tissue Eng Regen Med, 2019, 13, 385
DOI: 10.1002/term.2793

Mathematical Modeling of Neuronal Logic, Memory and Clocking Circuits

Stephen Lynch, Jon Borresen, Paul Roach, Mark Kotter and Mark Slevin

Int. J. Bifurcation Chaos, 2020, 30, 2050003
DOI: 10.1142/S0218127420500030