Issue 8, 2021

Histidine orientation in artificial peroxidase regioisomers as determined by paramagnetic NMR shifts

Abstract

Fe-Mimochrome VI*a is a synthetic peroxidase and peroxygenase, featuring two different peptides that are covalently-linked to deuteroheme. To perform a systematic structure/function correlation, we purposely shortened the distance between the distal peptide and the heme, allowing for the separation and characterization of two regioisomers. They differ in both His axial-ligand orientation, as determined by paramagnetic NMR shifts, and activity. These findings highlight that synthetic metalloenzymes may provide an efficient tool for disentangling the role of axial ligand orientation over peroxidase activity.

Graphical abstract: Histidine orientation in artificial peroxidase regioisomers as determined by paramagnetic NMR shifts

Supplementary files

Article information

Article type
Communication
Submitted
06 okt. 2020
Accepted
22 des. 2020
First published
22 des. 2020

Chem. Commun., 2021,57, 990-993

Histidine orientation in artificial peroxidase regioisomers as determined by paramagnetic NMR shifts

O. Maglio, M. Chino, C. Vicari, V. Pavone, R. O. Louro and A. Lombardi, Chem. Commun., 2021, 57, 990 DOI: 10.1039/D0CC06676A

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