Nucleus-targeted protein delivery via lysine dendron conjugation

Abstract

This study introduces lysine dendron (LD)-mediated protein conjugation for nucleus-targeted delivery. Using click chemistry, multivalent LD–protein architectures enhance cellular uptake and nuclear accumulation without extensive surface engineering. LD-modified RNase A shows superior nuclear delivery and antitumor efficacy in vitro/in vivo. This bioreversible strategy overcomes traditional limitations, offering a versatile platform for next-generation protein therapeutics.