Current trends in colorimetric biosensors using nanozymes for detecting biotoxins (bacterial food toxins, mycotoxins, and marine toxins)

Abstract

Biotoxins, predominantly bacterial food toxins, mycotoxins, and marine toxins, have emerged as major threats in the fields of seafood, other foods, feeds, and medicine. They have potential teratogenic, mutagenic, and carcinogenic effects on humans, occasionally triggering high morbidity and mortality. One of the apparent concerns relates to the increasing consumption of fast food resulting in the demand for processed food without adequate consideration of the toxins they may contain. Therefore, developing improved methods for detecting biotoxins is of paramount significance. Nanozymes, a type of nanomaterials exhibiting enzyme-like activity, are increasingly being recognized as viable alternatives to natural enzymes owing to their benefits, such as customizable design, controlled catalytic performance, excellent biocompatibility, and superior stability. The remarkable catalytic activity of nanozymes has led to their broad utilization in the development of colorimetric biosensors. This has emerged as a potent and efficient approach for rapid detection, enabling the creation of innovative colorimetric sensing methodologies through the integration of nanozymes with colorimetric sensors. In this review, recent development in nanozyme research and their application in colorimetric biosensing of biotoxins are examined with an emphasis on their characteristics and performance. The study particularly focuses on the peroxidase (POD) activity, oxidase (OXD) activity, superoxide dismutase (SOD), and catalase (CAT) activity of nanozymes in colorimetric biosensors. Ultimately, the challenges and future prospects of these assays are explored.