Issue 22, 2023

Organelle-targeting ratiometric fluorescent probes: design principles, detection mechanisms, bio-applications, and challenges

Abstract

Biological species, including reactive oxygen species (ROS), reactive sulfur species (RSS), reactive nitrogen species (RNS), F, Pd2+, Cu2+, Hg2+, and others, are crucial for the healthy functioning of cells in living organisms. However, their aberrant concentration can result in various serious diseases. Therefore, it is essential to monitor biological species in cellular organelles such as the cell membrane, mitochondria, lysosome, endoplasmic reticulum, Golgi apparatus, and nucleus. Among various fluorescent probes for species detection within the organelles, ratiometric fluorescent probes have drawn special attention as a potential way to get beyond the drawbacks of intensity-based probes. This method depends on measuring the intensity change of two emission bands (caused by an analyte), which produces an efficient internal referencing that increases the detection's sensitivity. This review article discusses the literature publications (from 2015 to 2022) on organelle-targeting ratiometric fluorescent probes, the general strategies, the detecting mechanisms, the broad scope, and the challenges currently faced by fluorescent probes.

Graphical abstract: Organelle-targeting ratiometric fluorescent probes: design principles, detection mechanisms, bio-applications, and challenges

Article information

Article type
Review Article
Submitted
23 feb. 2023
Accepted
27 apr. 2023
First published
12 maí 2023
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2023,14, 5842-5871

Organelle-targeting ratiometric fluorescent probes: design principles, detection mechanisms, bio-applications, and challenges

M. K. Goshisht, N. Tripathi, G. K. Patra and M. Chaskar, Chem. Sci., 2023, 14, 5842 DOI: 10.1039/D3SC01036H

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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