AIE-nanoparticle assisted ultra-deep three-photon microscopy in the in vivo mouse brain under 1300 nm excitation

Dongyu Li; Hequn Zhang; Lina L. Streich; Yalun Wang; Ping Lu; Ling Wang; Robert Prevedel; Jun Qian

doi:10.1039/D1QM00243K

AIE-nanoparticle assisted ultra-deep three-photon microscopy in the in vivo mouse brain under 1300 nm excitation

Dongyu Li,

†^abcd Hequn Zhang,†^be Lina L. Streich,^cf Yalun Wang,^g Ping Lu,

^h Ling Wang,^c Robert Prevedel

*^c and Jun Qian

*^b

Author affiliations

* Corresponding authors

^a Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, Hubei, China

^b State Key Laboratory of Modern Optical Instrumentations, Centre for Optical and Electromagnetic Research, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou, China
E-mail: qianjun@zju.edu.cn

^c Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Heidelberg 69126, Germany
E-mail: prevedel@embl.de

^d MoE Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, Wuhan, Hubei, China

^e Zhejiang University Interdisciplinary Institute of Neuroscience and Technology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China

^f Candidate for Joint PhD Degree from EMBL and Heidelberg University, Faculty of Biosciences, Heidelberg 69126, Germany

^g Zhejiang Gongshang Univ, Sch Informat & Elect Engn, Hangzhou 310018, Zhejiang, China

^h State Key Lab of Supramolecular Structure and Materials, Jilin University, Changchun 130012, China

Abstract

Aggregation-induced emission nanoparticles serve as promising fluorescence probes for multi-photon excitation microscopy due to the large absorption cross-section in the NIR-IIb region. Here we present organic AIE nanoparticles that feature a high absorption cross-section under three-photon excitation. We show that these enable ultra-deep NIR-IIa excited three-photon imaging in the in vivo mouse brain.

This article is part of the themed collection: FOCUS: Recent progress on aggregation-induced emission

Article information

https://doi.org/10.1039/D1QM00243K

Article type

Research Article

Submitted

10 feb. 2021

Accepted

25 feb. 2021

First published

25 feb. 2021

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Mater. Chem. Front., 2021,5, 3201-3208

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AIE-nanoparticle assisted ultra-deep three-photon microscopy in the in vivo mouse brain under 1300 nm excitation

D. Li, H. Zhang, L. L. Streich, Y. Wang, P. Lu, L. Wang, R. Prevedel and J. Qian, Mater. Chem. Front., 2021, 5, 3201 DOI: 10.1039/D1QM00243K

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Materials Chemistry Frontiers

AIE-nanoparticle assisted ultra-deep three-photon microscopy in the in vivo mouse brain under 1300 nm excitation

Abstract

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AIE-nanoparticle assisted ultra-deep three-photon microscopy in the in vivo mouse brain under 1300 nm excitation

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