Issue 16, 2020

Graphene–nucleic acid biointerface-engineered biosensors with tunable dynamic range

Abstract

Programmed biosensors with tunable quantification range and sensitivity would greatly broaden their application in medical diagnosis, food safety and environmental analysis. Herein, we proposed a graphene–nucleic acid biointerface-engineered biosensor, allowing target molecules to be detected with adjustable dynamic ranges and sensitivities. The biosensors were programmed by simply tuning the poly A tail of aptamer probes. The tuning of the poly A tail would allow the interaction between aptamer probes and graphene oxide (GO) to be modulated, in turn programing the competitive binding processes of aptamer probes to target molecules and GO. The biosensors, termed affinity-tunable aptasensors (atAptasensors) could be easily tuned with different dynamic ranges by using aptamer probes with different tail lengths, and the dynamic range could be extended to be over 3 orders by a combined use of multiple aptamer probes. Remarkably, the specificity of aptamer probes could be increased by increasing the interaction between aptamer probes and GO. Reliability of atAptasensor for ATP detection was tested in serum and milk samples, and we also applied atAptasensor for culture-independent analysis of microorganism pollution.

Graphical abstract: Graphene–nucleic acid biointerface-engineered biosensors with tunable dynamic range

  • This article is part of the themed collection: Biosensors

Supplementary files

Article information

Article type
Paper
Submitted
28 okt. 2019
Accepted
17 des. 2019
First published
20 des. 2019

J. Mater. Chem. B, 2020,8, 3623-3630

Graphene–nucleic acid biointerface-engineered biosensors with tunable dynamic range

Z. Zhao, H. Yang, W. Zhao, S. Deng, K. Zhang, R. Deng, Q. He, H. Gao and J. Li, J. Mater. Chem. B, 2020, 8, 3623 DOI: 10.1039/C9TB02388G

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