Issue 19, 2019

High resolution tracking of macrophage cells in deep organs and lymphatics using fluorescent polymer dots

Abstract

In vivo cell tracking can provide information on cell migration and accumulation in the organs. Here, both folate and amino modified polymer dots were synthesized and screened for in vitro and in vivo tracking of macrophage Ana-1 cells. Flow cytometry analysis demonstrated that prepared polymer dots showed cellular uptake of approximately 98% within a short incubation time of 2 h, and these polymer dots maintained a cell labeling rate over 97% after 2 d. Moreover, a CCK-8 assay suggested that these polymer dots increased Ana-1 cell viabilities up to 110% at concentrations from 5 to 50 μg mL−1. Furthermore, the in vivo real time imaging of labelled Ana-1 cells in the alveolus of lung and lymph nodes were clearly detected by probe-based confocal laser endomicroscopy (pCLE). This study demonstrates a unique approach using polymer dots for real-time high resolution tracking of macrophage cells in deep organs and the lymphatic system.

Graphical abstract: High resolution tracking of macrophage cells in deep organs and lymphatics using fluorescent polymer dots

Supplementary files

Article information

Article type
Paper
Submitted
05 feb. 2019
Accepted
03 apr. 2019
First published
09 apr. 2019
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2019,9, 10966-10975

High resolution tracking of macrophage cells in deep organs and lymphatics using fluorescent polymer dots

S. Tang, Y. Guo, Y. Yang, Y. Li, Y. Gao, C. Zhang and L. Xiong, RSC Adv., 2019, 9, 10966 DOI: 10.1039/C9RA00954J

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