Issue 56, 2018

Ultrasensitive fluorescent aptasensor for MUC1 detection based on deoxyribonuclease I-aided target recycling signal amplification

Abstract

A novel sensing strategy for sensitive detection of mucin 1 protein (MUC1) based on deoxyribonuclease I-aided target recycling signal amplification was proposed. In this paper, in the absence of MUC1, the MUC1 aptamer is absorbed on the surface of graphene oxide (GO) via π-stacking interactions. This results in quenching of the fluorescent label and no fluorescence signal is observed. Upon adding MUC1, the probe sequences could be specifically recognized by MUC1, leading to an increase in the fluorescence intensity. The detection limit is as low as 10 pg mL−1, and a linear range from 50 pg mL−1 to 100 ng mL−1. The assay is specific and sensitive, and successfully applied to the determination of MUC1 in spiked human serum, urine and saliva. Importantly, the proposed aptasensing strategy has great potential in detecting various protein and even cancer cells.

Graphical abstract: Ultrasensitive fluorescent aptasensor for MUC1 detection based on deoxyribonuclease I-aided target recycling signal amplification

Supplementary files

Article information

Article type
Paper
Submitted
02 ágú. 2018
Accepted
09 sep. 2018
First published
14 sep. 2018
This article is Open Access
Creative Commons BY license

RSC Adv., 2018,8, 32009-32015

Ultrasensitive fluorescent aptasensor for MUC1 detection based on deoxyribonuclease I-aided target recycling signal amplification

J. Zhang, F. Ran, W. Zhou, B. Shang, F. Yu, L. Wu, W. Hu, X. He and Q. Chen, RSC Adv., 2018, 8, 32009 DOI: 10.1039/C8RA06498A

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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