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A tool for the selective sequestration of ATP and PPi to aid in-solution phosphopeptide detection assays†

Eugenia Duodu,‡a   Dziyana Kraskouskaya,‡a   Rodolfo F. Gómez-Biagia  and  Patrick T. Gunning*a  
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* Corresponding authors

a Department of Chemistry and Department of Chemical and Physical Sciences, University of Toronto, Mississauga, 3359 Mississauga Road North, Mississauga, Ontario, Canada
E-mail: patrick.gunning@utoronto.ca

Abstract

The presence of small phospho-anions, such as PPi and ATP in protein samples often complicates the robust detection of phosphoproteins by metal-based chemosensors and receptors. We herein report the development of a bis(Zn2+-cyclen)-triethylbenzene scaffold which can selectively sequester PPi and ATP without affecting the detection of a di-phosphorylated peptide by a ProxyPhos chemosensor.

Graphical abstract: A tool for the selective sequestration of ATP and PPi to aid in-solution phosphopeptide detection assays

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Article information

https://doi.org/10.1039/C5AN01414J
Submitted
14 júl. 2015
Accepted
02 des. 2015
First published
02 des. 2015
Analyst, 2016,141, 820-822
Article type
Communication
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A tool for the selective sequestration of ATP and PPi to aid in-solution phosphopeptide detection assays

E. Duodu, D. Kraskouskaya, R. F. Gómez-Biagi and P. T. Gunning, Analyst, 2016, 141, 820
DOI: 10.1039/C5AN01414J

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