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Issue 40, 2013
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Surface imprinting of pepsin viaminiemulsion polymerization

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Abstract

Surface imprinted polymers allow accessibility of the selective binding sites to large molecules such as proteins. In this work, small polymer particles offering a large surface area were prepared via miniemulsion polymerization in the presence of pepsin serving as a template molecule. The influence of four different functional monomers and of the amount of the template on the imprinting effect of pepsin was investigated. After the miniemulsion polymerization and a washing step, stable polymer suspensions with an average particle diameter of 400–600 nm and a specific surface area of 30–65 m2 g−1 were obtained. The results of detailed rebinding experiments revealed that the highest imprinting effect was achieved with (3-acrylamidopropyl)trimethylammonium chloride as a functional monomer and a high amount of the template. These polymer particles also showed selectivity for pepsin against various proteins. This approach provides a fundamental step towards the development of synthetic protein receptors and protein scavenger materials useful in biomimetic assays and for clean-up in biotechnology.

Graphical abstract: Surface imprinting of pepsin via miniemulsion polymerization

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Article information


Submitted
31 maí 2013
Accepted
06 ágú. 2013
First published
07 ágú. 2013

J. Mater. Chem. B, 2013,1, 5489-5495
Article type
Paper

Surface imprinting of pepsin via miniemulsion polymerization

B. Pluhar, U. Ziener and B. Mizaikoff, J. Mater. Chem. B, 2013, 1, 5489
DOI: 10.1039/C3TB20773K

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