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Issue 6, 2013
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Competitive stem cell recruitment by multiple cytotactic cues

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A multitude of cytotactic cues direct cell migration in development, cancer metastasis and wound healing. However, our understanding of cell motility remains fragmented partially because current migration devices only allow the study of independent factors. We developed a cell motility assay that allows competitive recruitment of a given cell population simultaneously by gradients of multiple cytotactic cues, observable under real-time imaging. Well-defined uniform gradients of cytotactic cues can be independently generated and sustained in each channel. As a case study, bone marrow mesenchymal stem/stromal cells (MSCs) were exposed to 15 cytokines that are commonly present in arthritis. Cytokines that induced robust recruitment of MSCs in multiple groups were selected to ‘compete’ in a final round to yield the most chemotactic factor(s) based on cell migration numbers, distances, migration indices and motility over time. The potency of a given cytokine in competition frequently differed from its individual action, substantiating the need to test multiple cytokines concurrently due to synergistic or antagonistic effects. This new device has the rare capacity to screen molecules that induce cell migration in cancer therapy, drug development and tissue regeneration.

Graphical abstract: Competitive stem cell recruitment by multiple cytotactic cues

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The article was received on 19 apr. 2012, accepted on 03 jan. 2013 and first published on 31 jan. 2013

Article type: Paper
DOI: 10.1039/C2LC41219E
Lab Chip, 2013,13, 1156-1164

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    Competitive stem cell recruitment by multiple cytotactic cues

    A. Mendelson, Y. K. Cheung, K. Paluch, M. Chen, K. Kong, J. Tan, Z. Dong, S. K. Sia and J. J. Mao, Lab Chip, 2013, 13, 1156
    DOI: 10.1039/C2LC41219E

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