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Issue 19, 2013
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Determination of uric acid in human plasma and urine by microemulsion electrokinetic chromatography

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Abstract

A novel nonionic microemulsion electrokinetic chromatography (MEEKC) method has been developed and validated for the determination of uric acid. The optimum microemulsion consisted of 80 mmol L−1 Brij-35, 2% (v/v) n-heptane, 14% (v/v) 1-butanol, 2.5% (v/v) acetonitrile and 10 mmol L−1 sodium borate buffer (pH 9.25). Under the optimum conditions, the method had a good linear relationship (correlation coefficient: 0.9996) with a range of 5–200 μg mL−1 for uric acid. The limit of detection for uric acid was 0.50 μg mL−1. The intra-day RSD values for migration time and peak area ratio were less than 0.71% and 0.78% (n = 5), and inter-day values were less than 1.64% and 1.82% (n = 5), separately. The method was applied for the quantitation of uric acid in gout patients' plasma and urine with recoveries in the range of 99.84–119.36% and 97.15–108.48%, respectively.

Graphical abstract: Determination of uric acid in human plasma and urine by microemulsion electrokinetic chromatography

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Article information


Submitted
17 mar. 2013
Accepted
30 júl. 2013
First published
31 júl. 2013

Anal. Methods, 2013,5, 5201-5207
Article type
Paper

Determination of uric acid in human plasma and urine by microemulsion electrokinetic chromatography

X. Lin, Y. Wang, J. Sun, D. Xiao, W. Luo and H. Li, Anal. Methods, 2013, 5, 5201
DOI: 10.1039/C3AY40439K

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