Issue 12, 2024

Determination of trace morphine and its metabolites in mouse urine using a TpBD functionalized bivalve magnetic nano-adsorbent

Abstract

In this study, a new type of covalent organic framework (TpBD) functionalized bivalved magnetic microsphere (TpBD-DS MNS) adsorbent was applied for the enrichment and detection of trace morphine and its metabolites in mouse urine. The main factors affecting the efficiency of magnetic solid phase extraction were optimized, and the optimal MSPE conditions were obtained. Combined with the UPLC-MS/MS technique, a new method for determining trace morphine and its metabolites in urine was established. The detection (LOD) and quantification (LOQ) limits for morphine and its metabolites ranged from 0.16 pg mL−1 to 0.53 pg mL−1 and 0.26 pg mL−1 to 1.25 pg mL−1, respectively. The recovery of the methods ranged from 87.4–97.3%, and the RSD was less than 5%. By employing this methodology, we successfully obtained the temporal change curve of morphine and its metabolites in mouse urine through collection and measurement post intravenous administration of morphine. This approach not only presents a novel means for investigating pharmacokinetics and drug monitoring but also demonstrates significant potential in the fields of forensic toxicology and drug abuse surveillance.

Graphical abstract: Determination of trace morphine and its metabolites in mouse urine using a TpBD functionalized bivalve magnetic nano-adsorbent

Article information

Article type
Paper
Submitted
13 jan. 2024
Accepted
14 feb. 2024
First published
15 feb. 2024

Anal. Methods, 2024,16, 1748-1755

Determination of trace morphine and its metabolites in mouse urine using a TpBD functionalized bivalve magnetic nano-adsorbent

Y. Wang, X. Chen, W. Qian, T. Chen, S. Zhang and W. Zhang, Anal. Methods, 2024, 16, 1748 DOI: 10.1039/D4AY00080C

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