Issue 15, 2024

A chemiluminescence immunoassay for type IV collagen as a promising marker for liver fibrosis and cirrhosis

Abstract

Herein, a magnetic bead-based chemiluminescence assay is reported to detect type IV collagen (col-IV) in serum samples. Magnetic beads (MBs) exhibit biocompatibility. Taking advantage of this property, they were conjugated with the col-IV antibody. For the determination of col-IV, the interaction of the col-IV sample, anti-(col-IV)-alkaline phosphatase (anti-(col-IV)-ALP) and anti-col-IV-magnetic beads (anti-(col-IV)-MBs) was performed to generate chemiluminescence. Under the optimized conditions, the developed method displayed good linearity in the concentration range of 20–2000 ng mL−1 with the limit of 0.79 ng mL−1. The repeatability coefficient of variation (CV) for col-IV detection ranged from 3.16% to 7.50%. The col-IV level in samples collected from a hospital was assessed by the chemiluminescence assay. Satisfactory recoveries were obtained ranging from 93.30% to 100.14%. In conclusion, the magnetic bead-based chemiluminescence assay may be used as a routine and efficient tool to detect type IV collagen in clinical diagnosis.

Graphical abstract: A chemiluminescence immunoassay for type IV collagen as a promising marker for liver fibrosis and cirrhosis

Article information

Article type
Paper
Submitted
15 des. 2023
Accepted
27 feb. 2024
First published
26 mar. 2024

Anal. Methods, 2024,16, 2248-2255

A chemiluminescence immunoassay for type IV collagen as a promising marker for liver fibrosis and cirrhosis

X. Fu, F. Zhang, F. Zhen, L. Duan, J. Zhou and J. Ma, Anal. Methods, 2024, 16, 2248 DOI: 10.1039/D3AY02240D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements