Issue 23, 2018

SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range

Abstract

We designed a genetically encoded ratiometric fluorescent probe, SypHer3s, with enhanced brightness and optimized pKa, which responds to pH changes in different cellular compartments. SypHer3s was successfully utilized for imaging the pH dynamics in mitochondria of living neurons and in quantitative pH measurement in zebrafish embryos.

Graphical abstract: SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range

Supplementary files

Article information

Article type
Communication
Submitted
15 nóv. 2017
Accepted
20 feb. 2018
First published
20 feb. 2018

Chem. Commun., 2018,54, 2898-2901

SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range

Y. G. Ermakova, V. V. Pak, Y. A. Bogdanova, A. A. Kotlobay, I. V. Yampolsky, A. G. Shokhina, A. S. Panova, R. A. Marygin, D. B. Staroverov, D. S. Bilan, H. Sies and V. V. Belousov, Chem. Commun., 2018, 54, 2898 DOI: 10.1039/C7CC08740C

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