SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range

Y. G. Ermakova; V. V. Pak; Y. A. Bogdanova; A. A. Kotlobay; I. V. Yampolsky; A. G. Shokhina; A. S. Panova; R. A. Marygin; D. B. Staroverov; D. S. Bilan; H. Sies; V. V. Belousov

doi:10.1039/C7CC08740C

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SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range

Y. G. Ermakova, V. V. Pak, Y. A. Bogdanova, A. A. Kotlobay, I. V. Yampolsky, A. G. Shokhina, A. S. Panova, R. A. Marygin, D. B. Staroverov, D. S. Bilan, H. Sies and V. V. Belousov
Chem. Commun., 2018,54, 2898-2901
DOI: 10.1039/C7CC08740C, Communication

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Abstract | Cited by

We designed a genetically encoded ratiometric fluorescent probe, SypHer3s, with enhanced brightness and optimized pK_a, which responds to pH changes in different cellular compartments. SypHer3s was successfully utilized for imaging the pH dynamics in mitochondria of living neurons and in quantitative pH measurement in zebrafish embryos.

Graphical abstract: SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range

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