Issue 45, 2017

Immobilized enzymes: understanding enzyme – surface interactions at the molecular level

Abstract

Enzymes immobilized on solid supports have important and industrial and medical applications. However, their uses are limited by the significant reductions in activity and stability that often accompany the immobilization process. Here we review recent advances in our understanding of the molecular level interactions between proteins and supporting surfaces that contribute to changes in stability and activity. This understanding has been facilitated by the application of various surface-sensitive spectroscopic techniques that allow the structure and orientation of enzymes at the solid/liquid interface to be probed, often with monolayer sensitivity. An appreciation of the molecular interactions between enzyme and surface support has allowed the surface chemistry and method of enzyme attachement to be fine-tuned such that activity and stability can be greatly enhanced. These advances suggest that a much wider variety of enzymes may eventually be amenable to immobilization as green catalysts.

Graphical abstract: Immobilized enzymes: understanding enzyme – surface interactions at the molecular level

Article information

Article type
Review Article
Submitted
28 júl. 2017
Accepted
11 sep. 2017
First published
13 sep. 2017

Org. Biomol. Chem., 2017,15, 9539-9551

Immobilized enzymes: understanding enzyme – surface interactions at the molecular level

M. Hoarau, S. Badieyan and E. N. G. Marsh, Org. Biomol. Chem., 2017, 15, 9539 DOI: 10.1039/C7OB01880K

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