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A POCT assay based on commercial HCG strip for miRNA21 detection by integrating with RCA-HCR cascade amplification and CRISPR/Cas12a

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Automated Microfluidic Nucleic Acid Detection Platform-Integrated RPA-T7-Cas13a for Pathogen Diagnosis

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Solid-Phase Collateral Cleavage System Based on CRISPR/Cas12 and Its Application toward Facile One-Pot Multiplex Double-Stranded DNA Detection

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Programmable Multiplexed Nucleic Acid Detection by Harnessing Specificity Defect of CRISPR‐Cas12a

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CaSilico: A versatile CRISPR package for in silico CRISPR RNA designing for Cas12, Cas13, and Cas14

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Engineering Entropy-Driven Nanomachine-Mediated Morphological Evolution of Anisotropic Silver Triangular Nanoplates for Colorimetric and Photothermal Biosensing

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A Novel General Chemistry Experiment for Freshmen: Transgenic Soybean Detection Based on Microfluidic Molecular Fluorescence Spectroscopy Analysis

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Multiplexed Lab-on-a-Chip Bioassays for Testing Antibodies against SARS-CoV-2 and Its Variants in Multiple Individuals

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Synthetic mismatches enable specific CRISPR-Cas12a-based detection of genome-wide SNVs tracked by ARTEMIS

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Review of CRISPR/Cas Systems on Detection of Nucleotide Sequences

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PCR Independent Strategy-Based Biosensors for RNA Detection

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Integrating CRISPR/Cas within isothermal amplification for point-of-Care Assay of nucleic acid

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Unlocking the Full Potential of Cas12a: Exploring the Effects of Substrate and Reaction Conditions on Trans-Cleavage Activity

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CRISPR/Cas Toolbox: Unveiling the secret weapon for SNP detection

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A PAM-free CRISPR/Cas12a ultra-specific activation mode based on toehold-mediated strand displacement and branch migration

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Enzyme-Assisted Nucleic Acid Amplification in Molecular Diagnosis: A Review

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CRISPR-microfluidics nexus: Advancing biomedical applications for understanding and detection

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One-Pot Assay for Rapid Detection of Benzimidazole Resistance in Venturia carpophila by Combining RPA and CRISPR/Cas12a

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From Lab to Home: Ultrasensitive Rapid Detection of SARS-CoV-2 with a Cascade CRISPR/Cas13a-Cas12a System Based Lateral Flow Assay

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Visual single nucleotide polymorphism (SNP) detection for ALDH2 genotyping based on multiplex ligation probe amplification (MLPA) and lateral flow assay

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Rapid and accurate detection of SARS-CoV-2 mutations using a Cas12a-based sensing platform

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Dual toeholds regulated CRISPR-Cas12a sensing platform for ApoE single nucleotide polymorphisms genotyping

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Photoactivatable CRISPR/Cas12a Strategy for One-Pot DETECTR Molecular Diagnosis

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CRISPR-Cas-mediated diagnostics

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PAM-Independent CRISPR-Cas12a System for Specific Assays of Single Nucleotide Variants

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Harnessing the power of clustered regularly interspaced short palindromic repeats (CRISPR) based microfluidics for next-generation molecular diagnostics

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Application and development of CRISPR-Cas12a methods for the molecular diagnosis of cancer: A review

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The application of CRISPR-Cas in disease diagnosis and treatment

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Novel approaches in CRISPR/Cas12a-based sensing for HCC diagnosis - A review (2020–2025)

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Custom-Designed Probes for the Accurate Determination of Epidermal Growth Factor Receptor Mutations and Their Allelic Configuration

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Rapid detection of multiple SARS-CoV-2 variants of concern by PAM-targeting mutations

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Recent advances in CRISPR-based single-nucleotide fidelity diagnostics

K. A. V. Kohabir, E. A. Sistermans and R. M. F. Wolthuis

Commun Med, 2025, 5
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Detection of the Uveal Melanoma-Associated Mutation GNAQ Q209P from Liquid Biopsy Using CRISPR/Cas12a Technology

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Invader assay-induced catalytic assembly of multi-DNAzyme junctions for sensitive detection of single nucleotide polymorphisms

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Background-Free Rolling Circle Amplification for SERS Bioassay Using a Chimeric Hairpin-Integrated CRISPR/Cas12a System

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Detection and Discrimination of Single Nucleotide Polymorphisms by Quantification of CRISPR-Cas Catalytic Efficiency

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Ultrasensitive protein and exosome analysis based on a rolling circle amplification assisted-CRISPR/Cas12a strategy

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CRISPR-powered quantitative keyword search engine in DNA data storage

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ID-CRISPR: A CRISPR/Cas12a platform for label-free and sensitive detection of rare mutant alleles using self-interference DNA hydrogel reporter

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PNA-Pdx: Versatile Peptide Nucleic Acid-Based Detection of Nucleic Acids and SNPs

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Rapid and sensitive detection of nucleic acids using an RAA-CRISPR/Cas12b one-pot detection assay (Rcod)

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Automated Centrifugal Microfluidic Chip Integrating Pretreatment and Molecular Diagnosis for Hepatitis B Virus Genotyping from Whole Blood

Nan Zong, Ying Gao, Yong Chen, Xueqing Luo and Xingyu Jiang

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Miniaturization of CRISPR/Cas12-Based DNA Sensor Array by Non-Contact Printing

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Micromachines, 2024, 15, 144
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CRISPR-microfluidic array for single-copy DNA mini barcoding and rapid field species identification

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Sensors and Actuators B: Chemical, 2022, 359, 131567
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Detection of Antimalarial Resistance-Associated Mutations in Plasmodium falciparum via a Platform of Allele-Specific PCR Combined with a Gold Nanoparticle-Based Lateral Flow Assay

Weijia Cheng, Wei Wang, Huiyin Zhu, Xiaonan Song, Kai Wu, Jian Li and Jennifer L. Guler

Microbiol Spectr, 2022, 10
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CRISPR/Cas12a Collateral Cleavage-Driven Transcription Amplification for Direct Nucleic Acid Detection

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An Optimized CRISPR/Cas12a Assay to Facilitate the BRAF V600E Mutation Detection

Azadeh Etemadzadeh, Pouya Salehipour, Fatemeh Movahedi Motlagh, Masoomeh Khalifeh, Adnan Asadbeigi, Mina Tabrizi, Reza Shirkouhi and Mohammad Hossein Modarressi

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Advances in Clustered, Regularly Interspaced Short Palindromic Repeats (CRISPR)-Based Diagnostic Assays Assisted by Micro/Nanotechnologies

Huahua Yue, Mengqi Huang, Tian Tian, Erhu Xiong and Xiaoming Zhou

ACS Nano, 2021, 15, 7848
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In vivo processing of digital information molecularly with targeted specificity and robust reliability

Yangyi Liu, Yubin Ren, Jingjing Li, Fan Wang, Fei Wang, Chao Ma, Dong Chen, Xingyu Jiang, Chunhai Fan, Hongjie Zhang and Kai Liu

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Electrochemical genosensors on-a-chip: Applications in early diagnosis of pathogens

A. Dehghan, M.J. Kiani, A. Gholizadeh, J. Aminizadeh, A. Rahi, I. Zare, E. Pishbin and H. Heli

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Optimization and Clinical Application Potential of Single Nucleotide Polymorphism Detection Method Based on CRISPR/Cas12a and Recombinase Polymerase Amplification

Xingyue Wang, Ting Yang, Yunling Zhang, Zongyue Zeng, Qiang Wei, Pu Chen, Shuangshuang Yang, Yunfei Huang, Yongqi Zhang, Hongling Lu, Linhong Wu, Dijiao Tang, Ping Yang, Xuechun Wang, Qing Liu, Fan Li, Chao Ling and Shifeng Huang

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Single nucleotide polymorphism discrimination and genotyping based on cascade strand displacement reaction mediated label-free Cas12a system

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Sensors and Actuators B: Chemical, 2025, 423, 136832
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CRISPR-based, genotype-specific detection of yellow head virus genotype 1 with fluorescent, lateral flow and DNAzyme-assisted colorimetric readouts

Praphutson Aiamsa-at, Napawit Nonthakaew, Kornsunee Phiwsaiya, Saengchan Senapin and Thawatchai Chaijarasphong

Aquaculture, 2023, 574, 739696
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Gene point mutation information translation and detection: Leveraging single base extension and CRISPR/Cas12a

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Biosensors and Bioelectronics, 2024, 247, 115936
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A tiny sample rapid visual detection technology for imidacloprid resistance in Aphis gossypii by CRISPR/Cas12a

Rujing Kang, Ren Li, Juma Mjengi, Zohair Abbas, Yihong Song and Lei Zhang

Science of The Total Environment, 2024, 951, 175712
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Challenges and Opportunities for Clustered Regularly Interspaced Short Palindromic Repeats Based Molecular Biosensing

Mengdi Bao, Qun Chen, Zhiheng Xu, Erik C. Jensen, Changyue Liu, Jacob T. Waitkus, Xi Yuan, Qian He, Peiwu Qin and Ke Du

ACS Sens., 2021, 6, 2497
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Designing a CRISPR/Cas12a- and Au-Nanobeacon-Based Diagnostic Biosensor Enabling Direct, Rapid, and Sensitive miRNA Detection

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Detecting Melanocortin 1 Receptor Gene’s SNPs by CRISPR/enAsCas12a

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Genes, 2023, 14, 394
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Naked-eye on-site detection platform for Pasteurella multocida based on the CRISPR-Cas12a system coupled with recombinase polymerase amplification

Jie Hao, Longfei Xie, Tianmu Yang, Zhipeng Huo, Guifang Liu, Yahong Liu, Wenguang Xiong and Zhenling Zeng

Talanta, 2023, 255, 124220
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A Multiplexed Cas13-Based Assay with Point-of-Care Attributes for Simultaneous COVID-19 Diagnosis and Variant Surveillance

Maturada Patchsung, Aimorn Homchan, Kanokpol Aphicho, Surased Suraritdechachai, Thanyapat Wanitchanon, Archiraya Pattama, Khomkrit Sappakhaw, Piyachat Meesawat, Thanakrit Wongsatit, Artittaya Athipanyasilp, Krittapas Jantarug, Niracha Athipanyasilp, Juthamas Buahom, Supapat Visanpattanasin, Nootaree Niljianskul, Pimchai Chaiyen, Ruchanok Tinikul, Nuanjun Wichukchinda, Surakameth Mahasirimongkol, Rujipas Sirijatuphat, Nasikarn Angkasekwinai, Michael A. Crone, Paul S. Freemont, Julia Joung, Alim Ladha, Omar Abudayyeh, Jonathan Gootenberg, Feng Zhang, Claire Chewapreecha, Sittinan Chanarat, Navin Horthongkham, Danaya Pakotiprapha and Chayasith Uttamapinant

The CRISPR Journal, 2023, 6, 99
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One-pot RPA-Cas12a assay for instant and visual detection of Burkholderia pseudomallei

Ling Deng, Xiaoyi He, Ke Liu, Yuanli Li, Han Xia, Hang Qian, Xiaoxue Lu, Xuhu Mao and Yang Xiang

Analytica Chimica Acta, 2023, 1252, 341059
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Coordination chemistry in CRISPR-Cas-based point of care testing: A review of molecular probe development and applications

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Two‐Stage Mixed‐Dye‐Based Isothermal Amplification with Ribonuclease‐Cleavable Enhanced Probes for Dual‐Visualization Detection of SARS‐CoV‐2 Variants of Interest

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Advanced Science, 2024, 11
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CRISPR-Powered Microfluidics in Diagnostics: A Review of Main Applications

Mostafa Azimzadeh, Marziyeh Mousazadeh, Atieh Jahangiri-Manesh, Pouria Khashayar and Patricia Khashayar

Chemosensors, 2021, 10, 3
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CASMART, a one-step CRISPR Cas12a-mediated isothermal amplification for rapid and high-resolution digital detection of rare mutant alleles

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Navigating the CRISPR/Cas Landscape for Enhanced Diagnosis and Treatment of Wilson’s Disease

Woong Choi, Seongkwang Cha and Kyoungmi Kim

Cells, 2024, 13, 1214
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