Issue 8, 2021

Comparative evaluation and design of a G-triplex/thioflavin T-based molecular beacon

Abstract

Both G-quadruplex (G4) and G-triplex (G3) can bind thioflavin T (ThT) to light up the fluorescence of ThT. G4/ThT and G3/ThT can be used as fluorescent indicators to construct a label-free molecular beacon (MB). In this work, we present a comparative perspective of G3/ThT-based MB and G4/ThT-based MB. The results showed that the G3/ThT-based MB had higher sensitivity and faster response speed than the G4/ThT-based MB. Furthermore, we systematically studied the effect of stem length and varying pairs on the response of the G3/ThT-based MB, and then proposed one rational design of the G3/ThT-based MB. This work demonstrates that the shorter G3 is more suitable for constructing the MB stem. This present work opens a promising way to develop a sensitive, simple and homogeneous biosensing method.

Graphical abstract: Comparative evaluation and design of a G-triplex/thioflavin T-based molecular beacon

Supplementary files

Article information

Article type
Paper
Submitted
09 Փտվ 2021
Accepted
08 Մրտ 2021
First published
12 Մրտ 2021

Analyst, 2021,146, 2567-2573

Comparative evaluation and design of a G-triplex/thioflavin T-based molecular beacon

J. Gao, Q. Liu, W. Liu, Y. Jin and B. Li, Analyst, 2021, 146, 2567 DOI: 10.1039/D1AN00252J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements