Issue 12, 2021

A gold-based strip sensor for the detection of benzo[a]pyrene in edible oils

Abstract

This report describes the development of a sensitive and broadly specific indirect competitive enzyme-linked immunosorbent assay (icELISA) and a gold nanoparticle-based immunochromatographic strip (GNP-ICS) assay for the detection of benzo[a]pyrene (B[a]P), using an anti-B[a]P monoclonal antibody (mAb). A broad-specific anti-B[a]P mAb (4E8) was raised from two types of haptens, with half maximal inhibitory concentrations and limits of detection (LOD) values of 2.51 and 0.54 ng mL−1, respectively. In addition, the cross-reactivity was up to 390% with structurally related compounds. The GNP-ICS assay based on a GNP-labeled mAb showed broad specificity in the detection of B[a]P and its analogues, with cut-off and visual LOD values of 100 and 10 ng mL−1, respectively. Furthermore, the recoveries from the developed icELISA and GNP-ICS assay in edible oil samples spiked with B[a]P were validated by high-performance liquid chromatography–fluorescence detection. The results revealed that the icELISA could reliably detect B[a]P in edible oils.

Graphical abstract: A gold-based strip sensor for the detection of benzo[a]pyrene in edible oils

Supplementary files

Article information

Article type
Paper
Submitted
10 Ապր 2021
Accepted
06 Մյս 2021
First published
06 Մյս 2021

Analyst, 2021,146, 3871-3879

A gold-based strip sensor for the detection of benzo[a]pyrene in edible oils

J. Yao, X. Xu, L. Liu, H. Kuang, Z. Wang and C. Xu, Analyst, 2021, 146, 3871 DOI: 10.1039/D1AN00612F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements