Issue 2, 2017

Alkene hydrogenation activity of enoate reductases for an environmentally benign biosynthesis of adipic acid

Abstract

Adipic acid, a precursor for Nylon-6,6 polymer, is one of the most important commodity chemicals, which is currently produced from petroleum. The biosynthesis of adipic acid from glucose still remains challenging due to the absence of biocatalysts required for the hydrogenation of unsaturated six-carbon dicarboxylic acids to adipic acid. Here, we demonstrate the first enzymatic hydrogenation of 2-hexenedioic acid and muconic acid to adipic acid using enoate reductases (ERs). ERs can hydrogenate 2-hexenedioic acid and muconic acid producing adipic acid with a high conversion rate and yield in vivo and in vitro. Purified ERs exhibit a broad substrate spectrum including aromatic and aliphatic 2-enoates and a significant oxygen tolerance. The discovery of the hydrogenation activity of ERs contributes to an understanding of the catalytic mechanism of these poorly characterized enzymes and enables the environmentally benign biosynthesis of adipic acid and other chemicals from renewable resources.

Graphical abstract: Alkene hydrogenation activity of enoate reductases for an environmentally benign biosynthesis of adipic acid

Supplementary files

Article information

Article type
Edge Article
Submitted
27 jún. 2016
Accepted
08 okt. 2016
First published
11 okt. 2016
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2017,8, 1406-1413

Alkene hydrogenation activity of enoate reductases for an environmentally benign biosynthesis of adipic acid

J. C. Joo, A. N. Khusnutdinova, R. Flick, T. Kim, U. T. Bornscheuer, A. F. Yakunin and R. Mahadevan, Chem. Sci., 2017, 8, 1406 DOI: 10.1039/C6SC02842J

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