Issue 4, 2023

Supersensitive detection of single-histamine molecule on nanoplates by turn-on small molecule fluorescence sandwich immunoassay

Abstract

We develop a supersensitive “turn-on format” fluorescence sandwich immunoassay for detecting small single molecules. Gold nanoplate-based biotin antibodies and streptavidin–fluorophores were used instead of streptavidin–horseradish peroxidase reacting with a biotin tracer in a microplate-based competitive enzyme-linked immunosorbent assay (ELISA). Our platform showed a low detection limit of 5 zeptomolar (5 × 10–21 M), 5.4 × 1010 times higher detection sensitivity than the conventional tune-off format ELISA.

Graphical abstract: Supersensitive detection of single-histamine molecule on nanoplates by turn-on small molecule fluorescence sandwich immunoassay

Supplementary files

Article information

Article type
Communication
Submitted
22 okt. 2022
Accepted
20 jan. 2023
First published
20 jan. 2023

Analyst, 2023,148, 714-718

Supersensitive detection of single-histamine molecule on nanoplates by turn-on small molecule fluorescence sandwich immunoassay

S. Lee, J. Lee and S. H. Kang, Analyst, 2023, 148, 714 DOI: 10.1039/D2AN01734B

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