Luis E. Valencia, Zhicheng Zhang, Alexis J. Cepeda and Adrian T. Keatinge-Clay
Org. Biomol. Chem., 2019,17, 1375-1378
DOI:
10.1039/C8OB02858C,
Communication
Economical and environmentally-friendly routes to convert feedstock chemicals like acetate into valuable chiral products such as (R)-3-hydroxybutyrate are in demand. Here, seven enzymes (CoaA, CoaD, CoaE, ACS, BktB, PhaB, and GDH) are employed in a one-pot, in vitro, biocatalytic synthesis of (3R)-3-hydroxybutyryl-CoA, which was readily isolated. This platform generates not only chiral diketide building blocks but also desirable CoA derivatives.
