Sample preparation and detection methods in point-of-care devices towards future at-home testing

George Adedokun, Morteza Alipanah and Z. Hugh Fan

Lab Chip, 2024, 24, 3626
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Laser-induced graphene-based digital microfluidics (gDMF): a versatile platform with sub-one-dollar cost

Ke Liu, Yu He, Zefan Lu, Qiudi Xu, Lan Wang, Zhongxuan Liu, Jeremy Khou, Jiaming Ye, Chong Liu and Tao Zhang

Lab Chip, 2024, 24, 3125
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A sample-to-answer digital microfluidic multiplexed PCR system for syndromic pathogen detection in respiratory tract infection

Hao Bai, Jie Hu, Tangyuheng Liu, Liang Wan, Cheng Dong, Dasheng Luo, Fei Li, Zhanxin Yuan, Yunmei Tang, Tianlan Chen, Shan Wang, Hongna Gou, Yongzhao Zhou, Binwu Ying, Jin Huang and Wenchuang (Walter) Hu

Lab Chip, 2025, 25, 1552
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A point-of-care testing platform for on-site identification of genetically modified crops

Yangyang Wang, Furui Yang, Yingyi Fu, Xin He, Haowei Tian, Lili Yang, Mengxi Wu, Jijuan Cao and Junshan Liu

Lab Chip, 2024, 24, 2622
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Point-of-care analysis for foodborne pathogens in food samples based on a fully enclosed microfluidic chip cartridge

Dayun Tang, Peng Li, Shuo Qi, Qiong Wu, Ruili Yu, Mei Liu and Zhouping Wang

Lab Chip, 2025, 25, 3537
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Simultaneous detection of multiple spoilage bacteria using a fluorogenic loop-mediated isothermal amplification-based multi-sample microfluidic chip

Yirong Lou, Yuxin Chen, Qianjin Zhou, Jianmei Zhou, Zhongjie Xu, Wenjie Wang, Fangjie Chen, Hongxian Chen, Guizong Xu, Yanjun Zhang, Xizhi Shi and Jiong Chen

LWT, 2024, 193, 115758
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Universal portable colorimetric isothermal nucleic acid amplification equipment for immediate diagnosis

Kaihao Mao, Ye Tao, Wenshang Guo, Changrui Shi, Xiao Zhang, Ruizhe Yang, Rui Xue, Yongjun Sun and Yukun Ren

Sensors and Actuators B: Chemical, 2025, 427, 137218
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Machine learning-assisted image label-free smartphone platform for rapid segmentation and robust multi-urinalysis

Qianfeng Xu, Rongguo Yan, Xinrui Gui, Ruoyu Song and Xiaoli Wang

Anal Bioanal Chem, 2024, 416, 1443
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Open Thermal Control System for Stable Polymerase Chain Reaction on a Digital Microfluidic Chip

Jiajian Ji, Chenxuan Hu, Xinpei Pang, Jiancong Liang, Qi Huang, Siyi Hu, Qian Mei and Hanbin Ma

ACS Omega, 2024, 9, 10937
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A 3D-printed handheld microfluidic device for sensitive and low-cost on-site immunodetection

Fengyun Li, Jiebing Ai, Zhaowei Qu, Jianye Gao, Xiaoqin Ma, Yihuang Chen and Junping Yu

Microchim Acta, 2026, 193
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AIEgens-enhanced rapid sensitive immunofluorescent assay for SARS-CoV-2 with digital microfluidics

Yuping Zeng, Xiangyu Gan, Zhourui Xu, Xiaoxiang Hu, Chenxuan Hu, Hanbin Ma, Hangjia Tu, Bao Chai, Chengbin Yang, Siyi Hu and Yujuan Chai

Analytica Chimica Acta, 2024, 1298, 342398
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Integrated Microfluidic Chip for Neutrophil Extracellular Vesicle Analysis and Gastric Cancer Diagnosis

Dan Yu, Jianmei Gu, Jiahui Zhang, Maoye Wang, Runbi Ji, Chunlai Feng, Hélder A. Santos, Hongbo Zhang and Xu Zhang

ACS Nano, 2025, 19, 10078
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Multifunctional Edged‐Satellite AuAg Nanoparticles‐Based Integration Platform for Screening, Validation, and Elimination of Vibrio Bacteria

Yixuan Wu, Jiadong Chen, Liyan Bi, Zhiyang Zhang, Xiaoyan Wang, Longwen Fu, Qian Yang, Jaebum Choo and Lingxin Chen

Advanced Science, 2026, 13
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A phosphorylated guanidine chitosan and UiO-66-NH2 modified magnetic nanoparticle platform for enrichment and detection of multiple bacteria

Kexin Xu, Jinhui Zhu, Tong Zhang and Guodong Sui

Talanta, 2024, 278, 126435
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A portable all-in-one microfluidic device with real-time colorimetric LAMP for HPV16 and HPV18 DNA point-of-care testing

Hao Bai, Yuqing Liu, Linbo Gao, Tao Wang, Xiaoli Zhang, Jie Hu, Lisha Ding, Yueting Zhang, Qian Wang, Lei Wang, Jianlong Li, Zhifeng Zhang, Yang Wang, Chenlan Shen, Binwu Ying, Xiaoyu Niu and Wenchuang Hu

Biosensors and Bioelectronics, 2024, 248, 115968
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An integrated pH “Pen” for visual nucleic acid detection based on colorimetrical LAMP at point-of-care testing

Lei Wang, Zhifeng Wu, Jing Niu, Shangzhi Ji, Lulu Zhang, Michael G. Mauk, Fang Li and Xianbo Qiu

Anal Bioanal Chem, 2025, 417, 6611
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Nucleic acid amplification tests in digital microfluidics: the promise of next-generation point-of-care diagnostics

Duc Anh Thai and Yuguang Liu

Microsyst Nanoeng, 2025, 11
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AM‐DMF‐ddRPA: an All‐in‐One Digital Microfluidic Platform for Rapid and Automatic Digital Nucleic Acid Analysis

Jiajian Ji, Xinpei Pang, Chunyu Chang, Dongping Wang, Siyi Hu, Zhixin Fang, Chao Yu, Qian Mei and Hanbin Ma

Angewandte Chemie, 2025, 137
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Unveiling the potential of loop-mediated isothermal amplification (LAMP) for plant-parasitic nematode identification: a review

Gustavo Braga Babilônia, Everaldo Antônio Lopes, Maria Luiza Araújo Londe, Lara Bertoldo Ribeiro, Cláudio Marcelo Gonçalves Oliveira and Dalila Sêni Buonicontro

J Plant Dis Prot, 2024, 131, 665
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Rapid DNA extraction and microfluidic LAMP system in portable equipment for GM crops detection

Bin Xiao, Mengyu Wang, Juan Zhang, Nan Wang, Wei Fu, Hong Chen, Haoqian Wang, Liang Li, Xiangyi Pang, Chuan Liu, Fengchun Huang and Ailiang Chen

Sensors and Actuators B: Chemical, 2024, 411, 135716
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A power-free sample-loading microfluidic chip integrated with a colorimetric loop-mediated isothermal amplification and a chromatic analysis via image processing for multiplex detection of parasitic diseases

Wasin Panich, Chokchai Puttharugsa, Thanawan Tejangkura and Thapana Chontananarth

Analytica Chimica Acta, 2026, 1403, 345419
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MVPCR: Multiplex Visual Detection Strategy Based on Ultrafast PCR for Point-of-Care Pathogens Detection Within 10 Min

Ziqin Zhang, Cui Wu, Linlin Bai, Kaiming Guo, Ting Wang, Dianwei Liu, Mingrui Zhu, Feng Qian, Fang Zhang and Rui Wang

Phenomics, 2025, 5, 239
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Research on real-time nucleic acid detection device based on microfluidic technology

Shuo Wu, Jianxin Cheng, Xiaohua Cao, Jingdong Bo, Shilun Feng and Chuanjin Cui

International Journal on Smart Sensing and Intelligent Systems, 2025, 18
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An All-in-One Platform for On-Site Multiplex Foodborne Pathogen Detection Based on Channel-Digital Hybrid Microfluidics

Mei Xie, Tianlan Chen, Zongwei Cai, Bo Lei and Cheng Dong

Biosensors, 2024, 14, 50
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Recent advances and applications in integrating nucleic acid amplification technologies and CRISPR systems by spatial / temporal separation strategies for one-pot diagnosis

Di Huang, Yichen He, Chutian Xu, Peijie Shen, Shanshan Shi, Qiang Shu, Xiangming Fang and Zhinan Xu

TrAC Trends in Analytical Chemistry, 2025, 193, 118456
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Recent Advances in Loop-Mediated Isothermal Amplification (LAMP) for On-Site Virus Detection

Dain Kang, Sohyun Choi, Seokbeom Roh, Taeha Lee and Gyudo Lee

KSS, 2025, 34, 432
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Multi-Sample Detection of Soil Nitrate Nitrogen Using a Digital Microfluidic Platform

Yan Hong, Zhihao Xia, Jingming Su, Rujing Wang, Yongjia Chang, Qing Huang, Liman Wei and Xiangyu Chen

Agriculture, 2023, 13, 2226
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AM‐DMF‐ddRPA: an All‐in‐One Digital Microfluidic Platform for Rapid and Automatic Digital Nucleic Acid Analysis

Jiajian Ji, Xinpei Pang, Chunyu Chang, Dongping Wang, Siyi Hu, Zhixin Fang, Chao Yu, Qian Mei and Hanbin Ma

Angew Chem Int Ed, 2025, 64
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A cost-effective and field-deployable sensing system for chip-integrated detection of bacteria with the naked eye

Ren Shen, Wenhao Hui, Wenguang Wu, Ning Yang, Xiaodong Lin, Pui-In Mak, Rui P. Martins, Aiqun Liu and Yanwei Jia

Sensors and Actuators B: Chemical, 2024, 410, 135668
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Fundamentals and applications of digital microfluidics based on electrowetting-on-dielectric

Yuhan Chen, Xiaodong Zeng, Yingmei Shi, Haoqiang Feng, Mingliang Jin, Shuailong Zhang, Jieping Cao and Lingling Shui

Sensors and Actuators A: Physical, 2026, 401, 117623
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Integrated microfluidic cartridge for rapid and colorimetric detection of Mycoplasma pneumoniae

Jinling Cui, Binghui Shi, Mengnan Chai, Yanling Wang, Jiansen Du, Xiaoguang Lu, Chao Shi, Cuiping Ma and Yong Li

Microchim Acta, 2025, 192
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An Ultra-Compact and Low-Cost LAMP-Based Virus Detection Device

Dong Guo, Zhengrong Ling, Yifeng Tang, Gen Li, Tieshan Zhang, Haoxiang Zhao, Hao Ren, Yajing Shen and Xiong Yang

Sensors, 2024, 24, 4912
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In Situ Live Monitoring of Extracellular Acidosis near Cancer Cells Using Digital Microfluidics with an Integrated Optical pH Sensor Film

Wenting Qiu, Xuyan Lin and Stefan Nagl

Anal. Chem., 2024, 96, 14456
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Recent Advances in Nanomaterials for Enhanced Colorimetric Detection of Viruses and Bacteria

Caroline R. Basso, Marcos V. B. Filho, Victoria D. Gavioli, Joao P. R. L. L. Parra, Gustavo R. Castro and Valber A. Pedrosa

Chemosensors, 2025, 13, 112
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Bioinspired microfluidic chip for fluorescent detection of ultrafast and stable PCR

Long Huang, Bokai Luo, Jia Zhou and Lin Du

Eng. Res. Express, 2025, 7, 035573
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Lift-CM: An integrated lift-heater centrifugal microfluidic platform for point-of-care pathogen nucleic acid detection using isothermal amplification and CRISPR/Cas12a

Taowei Shu, Xueer Yin, Qiangyuan Xiong, Changyi Hua, Junjie Bu, Ke Yang, Jun Zhao, Yong Liu, Ling Zhu and Cancan Zhu

Biosensors and Bioelectronics, 2025, 274, 117178
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