SlipChip: from principle to applications

Yang Luo, Weijie Yuan, Sujin Jung and Feng Shen

Lab Chip, 2026, 26, 1250
DOI: 10.1039/D5LC01069A

A point-of-care testing platform for on-site identification of genetically modified crops

Yangyang Wang, Furui Yang, Yingyi Fu, Xin He, Haowei Tian, Lili Yang, Mengxi Wu, Jijuan Cao and Junshan Liu

Lab Chip, 2024, 24, 2622
DOI: 10.1039/D4LC00040D

Cleavable energy transfer labeled oligonucleotide probe for enhanced isothermal amplification detection and nano digital chip-based readout

Li Liu, Stephen J. Dollery, Gregory J. Tobin, Guoyu Lu and Ke Du

Nanoscale, 2025, 17, 1381
DOI: 10.1039/D4NR03142C

An all-in-one microfluidic SlipChip for power-free and rapid biosensing of pathogenic bacteria

Li Xue, Ming Liao and Jianhan Lin

Lab Chip, 2024, 24, 4039
DOI: 10.1039/D4LC00366G

Microfluidic platforms for CRISPR-based biosensing advancing molecular diagnostics from benchtop to point-of-care

Yanping Wang, Huimin Jiang, Yanyin Zhang, Qingran Yang, Yujun Song and Yanfeng Gao

Sens. Diagn., 2026, 5, 116
DOI: 10.1039/D5SD00176E

Recent progress in nucleic acid detection with CRISPR

Frank X. Liu, Johnson Q. Cui, Zhihao Wu and Shuhuai Yao

Lab Chip, 2023, 23, 1467
DOI: 10.1039/D2LC00928E

Opto-digital molecular analytics

Chelsea Violita Stanley, Yi Xiao, Tong Ling, Dong-Sheng Li and Peng Chen

Chem. Soc. Rev., 2025, 54, 3557
DOI: 10.1039/D5CS00023H

Droplet Digital CRISPR for Nucleic Acid Detection

Yang Zhang, Roy S. K. Walker, Anwar Sunna, Tracie J. Barber and Ming Li

Advanced Science, 2026, 13
DOI: 10.1002/advs.202517470

Loop-Mediated Isothermal Amplification-Integrated CRISPR Methods for Infectious Disease Diagnosis at Point of Care

Defne Yigci, Nazente Atçeken, Ali K. Yetisen and Savas Tasoglu

ACS Omega, 2023, 8, 43357
DOI: 10.1021/acsomega.3c04422

Digital Recombinase Polymerase Amplification, Digital Loop‐Mediated Isothermal Amplification, and Digital CRISPR‐Cas Assisted Assay: Current Status, Challenges, and Perspectives

Weihong Yin, Jianjian Zhuang, Jiale Li, Liping Xia, Kai Hu, Juxin Yin and Ying Mu

Small, 2023, 19
DOI: 10.1002/smll.202303398

Nucleic Acid Target Sensing Using a Vibrating Sharp-Tip Capillary and Digital Droplet Loop-Mediated Isothermal Amplification (ddLAMP)

Bethany J. Fike, Kathrine Curtin and Peng Li

Sensors, 2024, 24, 4266
DOI: 10.3390/s24134266

Recent Advances in Digital Biosensing Technology

Kathrine Curtin, Bethany J. Fike, Brandi Binkley, Toktam Godary and Peng Li

Biosensors, 2022, 12, 673
DOI: 10.3390/bios12090673

Digital single-molecule telomere length analysis via SlipChip-based real-time digital PCR platform

Yang Luo, Lei Xu, Weiyuan Lyu, Qi Wang, Xiao Zhang, Kai Qin and Feng Shen

Sensors and Actuators B: Chemical, 2026, 458, 139840
DOI: 10.1016/j.snb.2026.139840

CRISPR‐Cas Biochemistry and CRISPR‐Based Molecular Diagnostics

Zhengyan Weng, Zheng You, Jie Yang, Noor Mohammad, Mengshi Lin, Qingshan Wei, Xue Gao and Yi Zhang

Angewandte Chemie, 2023, 135
DOI: 10.1002/ange.202214987

Multiplex Digital Nucleic Acid Analysis by a LAMP–Argonaute Coupling Assay via a Parallel Droplet Fusion SlipChip

Yang Luo, Xingyu Ye, Nan Shen, Lei Xu, Jiajie Zhang, Zheyi Sheng, Qian Liu, Yan Feng and Feng Shen

Anal. Chem., 2025, 97, 731
DOI: 10.1021/acs.analchem.4c05145

CRISPR‐Cas Biochemistry and CRISPR‐Based Molecular Diagnostics

Zhengyan Weng, Zheng You, Jie Yang, Noor Mohammad, Mengshi Lin, Qingshan Wei, Xue Gao and Yi Zhang

Angew Chem Int Ed, 2023, 62
DOI: 10.1002/anie.202214987

Combination-Lock SlipChip Integrating Nucleic Acid Sample Preparation and Isothermal LAMP Amplification for the Detection of SARS-CoV-2

Jiajie Zhang, Lei Xu, Zheyi Sheng, Jiayi Zheng, Weiyu Chen, Qixin Hu and Feng Shen

ACS Sens., 2024, 9, 646
DOI: 10.1021/acssensors.3c01727

Ultrasensitive Nucleic Acid Testing: From Foundational Research to Clinical Translation

Bao Li, Xuanyu Gu, Wu Zeng, Baobao Lin, Xinhe Liu, Chwee Teck Lim and Peng Liu

ACS Nano, 2025, 19, 40304
DOI: 10.1021/acsnano.5c15324

Multiplex Digital Immunoassays with Reduced Pre-partition Reaction via Massively Parallel Reagent Delivery on a Bead-Based SlipChip

Weiyuan Lyu, Jingwei Yi, Qingsheng Guo, Yao Wang, Jiajie Zhang, Tong Sun, Haoji Ma, Tingting Tang, Xueling Quan, Hong Xu, Feng Shen and Hongchen Gu

ACS Nano, 2023, 17, 12641
DOI: 10.1021/acsnano.3c03044

Digital CRISPR systems for the next generation of nucleic acid quantification

Anthony J. Politza, Reza Nouri and Weihua Guan

TrAC Trends in Analytical Chemistry, 2023, 159, 116917
DOI: 10.1016/j.trac.2023.116917

Progress in Biosensors for the Point-of-Care Diagnosis of COVID-19

Miroslav Pohanka

Sensors, 2022, 22, 7423
DOI: 10.3390/s22197423

The Fantastic Single-Molecule Techniques

Huang Tang, Shuting Liu, Chenyue Kang, Xiang Wang, Xi Zhang, Kun Li, Gege Duan, Zheng Li, Boyang Hua and Xing-Hua Xia

Chemical & Biomedical Imaging, 2026, 4, 256
DOI: 10.1021/cbmi.5c00112

Digital CRISPR-based diagnostics for quantification of Candida auris and resistance mutations

Justin C. Rolando, Anton Thieme, Nicole E. Weckman, Nayoung Kim, Helena de Puig, Xiao Tan, Emily Cotnoir, Vishnu Chaturvedi, James J. Collins and David R. Walt

Nat. Biomed. Eng, 2026
DOI: 10.1038/s41551-025-01597-0

Digital Bioassays on the Slipchip Microfluidic Devices

Qingqing Luo, Ziqing Yu, Weiyuan Lyu, Yang Luo, Lei Xu and Feng Shen

Advanced Sensor Research, 2025, 4
DOI: 10.1002/adsr.202500030

A Microfluidic Chip-Based Integrated Device Combining Aerosol Sampling and LAMP–CRISPR Detection for Airborne Virus Surveillance

Anlan Zhang, Yuqing Chang, Wen Li, Yuanbao Zhang, Yuqian Wang, Haohan Xie, Tao Zuo, Yu Zhang, Jiyu Xi, Xin Wu, Zewen Wei and Rui Chen

Biosensors, 2025, 15, 475
DOI: 10.3390/bios15080475

From droplets to diagnosis: AI-driven imaging and system integration in digital nucleic acid amplification testing

Yuanyuan Wei, Xianxian Liu, Yao Mu, Changran Xu, Guoxun Zhang, Tianhao Li, Zida Li, Wu Yuan, Ho-Pui Ho and Mingkun Xu

Biosensors and Bioelectronics, 2025, 288, 117741
DOI: 10.1016/j.bios.2025.117741

Programmable droplet microfluidics for complex multistep bioassays

Enqi Huang, Yu Wang, Dongyang Cai and Dayu Liu

Interdisciplinary Medicine, 2024, 2
DOI: 10.1002/INMD.20230026

SlipChip Enables the Integration of CRISPR-Cas12a and RPA for Fast and Stand-Alone HPV Detection

Yin Zhao, Zheyu Li, Tao Li, Ruotong Rao, Jiang Zhu, Rui Hu, Guoyong Xu, Ying Li and Yunhuang Yang

Anal. Chem., 2024, 96, 20602
DOI: 10.1021/acs.analchem.4c05290

Challenges and perspectives of CRISPR-based technology for diagnostic applications

Stefano Del Giovane, Neda Bagheri, Andrea Celeste Di Pede, Alejandro Chamorro, Simona Ranallo, Davide Migliorelli, Loïc Burr, Samantha Paoletti, Hatice Altug and Alessandro Porchetta

TrAC Trends in Analytical Chemistry, 2024, 172, 117594
DOI: 10.1016/j.trac.2024.117594

CRISPR/Cas12a-Assisted isothermal amplification for rapid and specific diagnosis of respiratory virus on an microfluidic platform

Jienan Shen, Zhi Chen, Ruibin Xie, Jingfeng Li, Chunyan Liu, Yaqing He, Xiaopeng Ma, Hui Yang and Zhongjian Xie

Biosensors and Bioelectronics, 2023, 237, 115523
DOI: 10.1016/j.bios.2023.115523

Experimental investigation of confinement effect in single molecule amplification via real-time digital PCR on a multivolume droplet array SlipChip

Yang Luo, Qixin Hu, Yan Yu, Weiyuan Lyu and Feng Shen

Analytica Chimica Acta, 2024, 1304, 342541
DOI: 10.1016/j.aca.2024.342541

STAMP-Based Digital CRISPR-Cas13a for Amplification-Free Quantification of HIV-1 Plasma Viral Loads

Reza Nouri, Yuqian Jiang, Anthony J. Politza, Tianyi Liu, Wallace H. Greene, Yusheng Zhu, Jonathan J. Nunez, Xiaojun Lian and Weihua Guan

ACS Nano, 2023, 17, 10701
DOI: 10.1021/acsnano.3c01917

The nucleic acid detection using CRISPR/Cas biosensing system with micro-nano modality for point-of-care applications

Liang Zhao, Zihao Zhao, Ning Li and Xiayan Wang

Talanta, 2025, 286, 127457
DOI: 10.1016/j.talanta.2024.127457

An automated, dead-volume reduced, isothermal digital nucleic acid quantification system based on recombinase polymerase amplification

Zhongping Zhang, Rui Zhu, Tianwei Li, Nan Wang, Lulu Zhang, Michael G. Mauk, Guijun Miao and Xianbo Qiu

Microsyst Technol, 2025, 31, 2221
DOI: 10.1007/s00542-025-05850-z

Rapid Bacteriophage Quantification by Digital Biosensing on a SlipChip Microfluidic Device

Xiang Li, Qixin Hu, Xu Liu, Jianfang Liu, Nannan Wu and Feng Shen

Anal. Chem., 2023, 95, 8632
DOI: 10.1021/acs.analchem.3c01066

Development of chamber-based digital polymerase chain reaction: From chip to device

Jizhi Tong, Fangfang Yin, Mengda Liang, Wen Zong, Yu Wang, Duo Xiao, Juxin Yin and Ying Mu

TrAC Trends in Analytical Chemistry, 2025, 191, 118299
DOI: 10.1016/j.trac.2025.118299

Digitally fabricated 3D slippery architectures for multifunctional liquid manipulation

Woo Young Kim, Seong Min Yoon, Seo Rim Park, Myung Seo Kim, Sang Hoon Lee, Su Hyun Choi, Seungwoo Shin, Sin Kwon, Chang Jong Kim, Kwang Min Lee, Sang-Hoon Nam, Soochan Bae, Peter M. Kang, Nicholas X. Fang, Seok Kim and Young Tae Cho

Nat Commun, 2025, 16
DOI: 10.1038/s41467-025-64078-7