Regulating CRISPR/Cas12a trans-cleavage on the hairpin DNA–MB nanointerface for enhanced multiplexed sensing application

Chenxi Zhao, Lijie Du, Dike Jiang, Jing Hu and Xiandeng Hou

Chem. Sci., 2025, 16, 11456
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Intracellular biosensors by functional nanomaterial-integrated CRISPR technologies for real-time molecular sensing

Min Yu Choi, Chenzhong Li, Jin-Ha Choi and Jeong-Woo Choi

Chem. Commun., 2026, 62, 58
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A collagen-immobilized nanodevice for in situ ratiometric imaging of cancer biomarkers in the tumor microenvironment

Fengyu Tian, Shurui Zhou, Shiyi Xie, Zhenhua Zhang, Ling Peng, Ling Jiang, Zeyuan Wang, Zhou Nie and Yan Huang

Chem. Sci., 2023, 14, 12182
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Live-cell RNA imaging using the CRISPR-dCas13 system with modified sgRNAs appended with fluorescent RNA aptamers

Heng Tang, Junran Peng, Shuang Peng, Qi Wang, Xin Jiang, Xiaocheng Xue, Yanxin Tao, Limin Xiang, Quanjiang Ji, Song-Mei Liu, Xiaocheng Weng and Xiang Zhou

Chem. Sci., 2022, 13, 14032
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Amplification-free detection of telomerase activity at the single-cell level via Cas12a-lighting-up single microbeads (Cas12a-LSMBs)

Honghong Wang, Shuhui Wang, Hui Wang, Fu Tang, Desheng Chen, Yuanwen Liang and Zhengping Li

Lab Chip, 2023, 23, 4674
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A high-fidelity DNAzyme-assisted CRISPR/Cas13a system with single-nucleotide resolved specificity

Yunping Wu, Ruigang Jin, Yangyang Chang and Meng Liu

Chem. Sci., 2024, 15, 6934
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PAM-free CRISPR/Cas12a biosensor for PNAs-assisted isothermal amplification detection of S. typhimurium

He Sun, Shengjun Bu, Jiahong Wang, Zebin Zhang, Chenxi Li, Yue Wan, Yukun Zhao, Hongtao Zhou, Zhuo Hao, Xiuli Feng, Chang Li and Jiayu Wan

International Journal of Biological Macromolecules, 2025, 328, 147721
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Unlocking the Full Potential of Cas12a: Exploring the Effects of Substrate and Reaction Conditions on Trans-Cleavage Activity

Jie Xu, Zhujun Liu, Zhen Zhang and Tongbo Wu

Anal. Chem., 2023, 95, 10664
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PAM-free cascaded strand displacement coupled with CRISPR-Cas12a for amplified electrochemical detection of SARS-CoV-2 RNA

Kai Shi, Zhigang Yi, Yaoxia Han, Jiaxuan Chen, Yu Hu, Ying Cheng, Sujun Liu, Wei Wang and Jiuhua Song

Analytical Biochemistry, 2023, 664, 115046
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Exploring the Effect of Steric Hindrance on Trans-cleavage Activity of CRISPR-cas12a for Ultrasensitive SERS Detection of P53 DNA

Shixin Zhou, Jinzhuo Ran, Shanyou Man, Jiale Zhang, Ruo Yuan and Xia Yang

Anal. Chem., 2024, 96, 10654
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Dual miRNAs Imaging Platform Based on HRCA-Cas12a by Replacing PAM with Bubble to Reduce False Positive

Xiaojuan Wang, Chenxi Zhao, Nana Yin, Xue Wang, Yang Shu and Jianhua Wang

Anal. Chem., 2025, 97, 3053
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Photocontrolled Programmable Enzymatic Cascade for Robust CRISPR Diagnostics

Menglu Hu, Yihui Wang, Weiwei Qi, Yuqing Zhang, Jiachen Sun, Meng Cheng and Xiaoming Zhou

J. Am. Chem. Soc., 2025, 147, 31004
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Photoactivatable CRISPR/Cas12a Sensors for Biomarkers Imaging and Point-of-Care Diagnostics

Qing-Nan Li, Dong-Xia Wang, Dan-Ye Chen, Jia-Ao Lyu, Ya-Xin Wang, Shun-Li Wu, Hong-Xin Jiang and De-Ming Kong

Anal. Chem., 2024, 96, 2692
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Binding-driven forward tearing protospacer activated CRISPR-Cas12a system and applications for microRNA detection

Lina Zhao, Xiangyu Deng, Yuqing Li, Qing Zhao, Lizhu Xiao, Jianjiang Xue, Anyi Chen, Wei Cheng and Min Zhao

J Nanobiotechnol, 2024, 22
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CRISPR-Hg: Rapid and visual detection of Hg2+ based on PCR coupled with CRISPR/Cas12a

Fange Kong, Chunxia Wang, Shichao Peng, Zhengrui Chen, Yibing Huang, Jicheng Zhang, Jiasi Wang and Di Wang

Talanta, 2024, 277, 126379
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In vivo imaging of mitochondrial DNA mutations using an integrated nano Cas12a sensor

Yanan Li, Yonghua Wu, Ru Xu, Jialing Guo, Fenglei Quan, Yongyuan Zhang, Di Huang, Yiran Pei, Hua Gao, Wei Liu, Junjie Liu, Zhenzhong Zhang, Ruijie Deng, Jinjin Shi and Kaixiang Zhang

Nat Commun, 2023, 14
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miR-Cabiner: A Universal microRNA Sensing Platform Based on Self-Stacking Cascaded Bicyclic DNA Circuit-Mediated CRISPR/Cas12a

Ruijia Deng, Jing Sheng, Zuowei Xie, Hongzhao Yang, Sha Yang, Shuang Xie, Xiaoqi Tang, Shuang Zhao, Haohao Dong, Ming Chen and Kai Chang

Anal. Chem., 2025, 97, 799
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Novel non-nucleic acid targets detection strategies based on CRISPR/Cas toolboxes: A review

Xinkuan Cheng, Yaru Li, Jun Kou, Dan Liao, Wenlu Zhang, Lijuan Yin, Shuli Man and Long Ma

Biosensors and Bioelectronics, 2022, 215, 114559
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Telomerase-Responsive CRISPR System-Regulated Nanobomb for Triggering Research on Telomerase “Self-Detonation”

Wenyue Zhang, Ziyi Li, Xiaoli Lun and Yingshu Guo

ACS Appl. Mater. Interfaces, 2025, 17, 725
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Spatially blocked split CRISPR-Cas12a system for ultra-sensitive and versatile small molecule activation and detection

Hao Hu, Songcheng Guo, Yiyuan Li, Kejun Dong, Yan Lu, Keyi Ye, Longjie Li, Xiaoyu Zhou, Liming Cheng and Xianjin Xiao

Nat Commun, 2025, 16
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Nucleic Acid Enzyme‐Activated CRISPR‐Cas12a With Circular CRISPR RNA for Biosensing

Yunping Wu, Dingran Chang, Yangyang Chang, Qiang Zhang, Yi Liu, John D. Brennan, Yingfu Li and Meng Liu

Small, 2023, 19
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Sensitive detection of piRNAs using PAM-free CRISPR-Cas12a system integrated with primer extension amplification

Xiang Chen, Yan Yu, Zhigang Liang, Lulu Qin, Guanhong Xu, Fangdi Wei, Jing Yang, Qin Hu and Yao Cen

Sensors and Actuators B: Chemical, 2026, 453, 139473
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Engineering Anti‐CRISPR Proteins to Create CRISPR‐Cas Protein Switches for Activatable Genome Editing and Viral Protease Detection

Wenyuan Kang, Fei Xiao, Xi Zhu, Xinyu Ling, Shiyi Xie, Ruimiao Li, Peihang Yu, Linxin Cao, Chunyang Lei, Ye Qiu, Tao Liu and Zhou Nie

Angew Chem Int Ed, 2024, 63
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CRISPR/Cas bioimaging: From whole body biodistribution to single-cell dynamics

Ekaterina Kolesova, Sabina Pulone, Dmitry Kostyushev and Ennio Tasciotti

Advanced Drug Delivery Reviews, 2025, 224, 115619
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PAM-independent ultra-specific activation of CRISPR-Cas12a via sticky-end dsDNA

Wei Zhang, Yaoqin Mu, Kejun Dong, Lei Zhang, Bei Yan, Hao Hu, Yangwei Liao, Rong Zhao, Wan Shu, Zhengxin Ye, Yaping Lu, Chong Wan, Qiangqiang Sun, Longjie Li, Hongbo Wang and Xianjin Xiao

Nucleic Acids Research, 2022, 50, 12674
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Three novel Cas12a orthologs with robust DNA cleavage activity suitable for nucleic acid detection

Xueping Liu, Xiaotong Qiu, Lichao Han, Yuan Yue, Shuai Xu, Fang Li, Jiang Yao, Lina Sun and Zhenjun Li

Gene, 2023, 852, 147055
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Conformational transformation of activator strand directly regulates CRISPR/Cas12a system activity for live cell sensing of multiple biomolecules

Qing-Nan Li, Dong-Xia Wang, Zhi-Qi Dai, Shun-Li Wu, Gui-Mei Han, Sha Lu, Li-Na Zhu, Hong-Xin Jiang and De-Ming Kong

Chemical Engineering Journal, 2024, 494, 153084
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PAM-Independent CRISPR-Cas12a System for Specific Assays of Single Nucleotide Variants

Jinlong Ai, Jinhai Deng, Jingjing Hu, Xingxiang Pu, Tongyan Yuan, Yuling Teng, Han Li, Bojie Chen, Jinlian Du, Ling Jiang, Xiaoyan Chen, Erhu Xiong and Ronghua Yang

JACS Au, 2025, 5, 1392
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Recent advances and challenges of the use of the CRISPR/Cas system as a non-nucleic acid molecular diagnostic

Jian Zhou, Xue-mei Ren, Xin Wang, Zhuo Li and Cory J Xian

Heliyon, 2023, 9, e22767
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A Versatile Integrated Microfluidic Chip Based on Sonic Toothbrush-Assisted Mixing for Analyses of Diverse Biomolecules

Dongliu Xiang, Hui Wen, Hongmei Fang, Yuan Zhou, Lei Luo, Qing Wang, Xueting Yan, Jin Huang, Jianbo Liu, Xiaohai Yang and Kemin Wang

Anal. Chem., 2023, 95, 7416
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Rational design of CRISPR–Cas12a crRNAs for enhanced allelic discrimination and low-abundance variant detection

Wei Wu, Yijie Gong, Mengjun Deng, Manlu Yang, Lifeng Wang, Yuanyuan Wang, Li Yang, Xueping Chen, Guoming Xie and Junjie Li

Chemical Engineering Journal, 2026, 533, 174837
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CRISPR-Based Live Cell Biosensing: Innovations in Signal Labeling Strategies and Delivery Systems

Yao Yin, Zixuan Li, Can Liu, Xiao-Bing Zhang and Qishui Ou

ACS Sens., 2025, 10, 5456
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Kinetics Accelerated CRISPR-Cas12a Enabling Live-Cell Monitoring of Mn2+ Homeostasis

Shiyi Xie, Benfeng Xu, Rui Tang, Siyu Chen, Chunyang Lei and Zhou Nie

Anal. Chem., 2022, 94, 10159
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A switchable Cas12a enabling CRISPR-based direct histone deacetylase activity detection

Wenyuan Kang, Lin Liu, Peihang Yu, Tianyi Zhang, Chunyang Lei and Zhou Nie

Biosensors and Bioelectronics, 2022, 213, 114468
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Development and application of fluorescence RNA imaging tools

Peng YIN, JiaNan WANG, ChunZheng HUANG, Shi KUANG and Zhou NIE

Sci. Sin.-Vitae, 2025, 55, 1591
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CRISPR-Cas-Based Diagnostics in Biomedicine: Principles, Applications, and Future Trajectories

Zhongwu Zhou, Il-Hoon Cho and Ulhas S. Kadam

Biosensors, 2025, 15, 660
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Regulation of CRISPR trans-cleavage activity by an overhanging activator

Na Yin, Hongyan Yu, Li Zhang, Fei Luo, Weitao Wang, Xiaole Han, Yu He, Yiqi Zhang, You Wu, Jiu Pu, Tong Feng, Gang Yang, Tingmei Chen and Guoming Xie

Nucleic Acids Research, 2025, 53
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AND Logic-Gate-Based CRISPR/Cas12a Biosensing Platform for the Sensitive Colorimetric Detection of Dual miRNAs

Shaohua Gong, Xi Wang, Ping Zhou, Wei Pan, Na Li and Bo Tang

Anal. Chem., 2022, 94, 15839
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Exploring the effect of activator topology on CRISPR–Cas12a trans-cleavage activity

Zixuan Zhu, Xiaolong Li, Lin Ding and Tongbo Wu

Nucleic Acids Research, 2025, 53
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Light-activated CRISPR-Cas12a for amplified imaging of microRNA in cell cycle phases at single-cell levels

Hong Liu, Jiantong Dong, Zhijuan Duan, Fan Xia, Itamar Willner and Fujian Huang

Sci. Adv., 2024, 10
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Engineering stimuli-responsive CRISPR-Cas systems for versatile biosensing

Linxin Cao, Wenhui Chen, Wenyuan Kang, Chunyang Lei and Zhou Nie

Anal Bioanal Chem, 2025, 417, 1699
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Engineered RNA Devices for In Vivo Targeted Therapeutics via Advanced Delivery Systems

Wei Luo, Xia Liu, Ying Han, Yijing Duan, Chuao Yu, Na Kong and Tian Xie

Aggregate, 2025, 6
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Direct visualization of living bacterial genotypes using CRISPR/Cas12a-circular reporter nanoprobes

Yanan Li, Yiran Pei, Di Huang, Ru Xu, Yonghua Wu, Yaqiong Li, Ruixue Zhou, Yongbin Wei, Xiao Tan, Hua Gao, Jinjin Shi, Zhenzhong Zhang, Kaixiang Zhang and Jinghong Li

Biosensors and Bioelectronics, 2022, 216, 114641
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Allosteric ribozyme-driven crRNA switch for the amplification-free detection of biomolecules

Mei Su, Hong-Shuai Zhang, Hao Liu, Kai Yang and Zhan-Ming Ying

Biosensors and Bioelectronics, 2025, 280, 117450
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A PAM-free CRISPR/Cas12a ultra-specific activation mode based on toehold-mediated strand displacement and branch migration

You Wu, Wang Luo, Zhi Weng, Yongcan Guo, Hongyan Yu, Rong Zhao, Li Zhang, Jie Zhao, Dan Bai, Xi Zhou, Lin Song, Kena Chen, Junjie Li, Yujun Yang and Guoming Xie

Nucleic Acids Research, 2022, 50, 11727
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Construction of molecular logic gates using heavy metal ions as inputs based on catalytic hairpin assembly and CRISPR-Cas12a

Jiafeng Pan, Fang Deng, Zhi Liu, Lingwen Zeng and Junhua Chen

Talanta, 2023, 255, 124210
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Recent Advances in CRISPR/Cas-Based Biosensors for Protein Detection

Jing Wang, Xifang Yang, Xueliang Wang and Wanhe Wang

Bioengineering, 2022, 9, 512
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Engineering Anti‐CRISPR Proteins to Create CRISPR‐Cas Protein Switches for Activatable Genome Editing and Viral Protease Detection

Wenyuan Kang, Fei Xiao, Xi Zhu, Xinyu Ling, Shiyi Xie, Ruimiao Li, Peihang Yu, Linxin Cao, Chunyang Lei, Ye Qiu, Tao Liu and Zhou Nie

Angewandte Chemie, 2024, 136
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CRISPR/Cas9 in cancer research and therapeutics: Clinical applications, optimization strategies, and future perspectives

Alireza Shariati, Amirreza Homaei, Arya Khezrpour, Nima Azizi, Pouria Khani, Fatemeh Shariati, Maryam Rafiee, Hamed Afkhami, Mohammad Hasan Yousefi, Farzane Saeidi and Mohammad Hossein Modarressi

Current Research in Biotechnology, 2026, 100382
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CRISPR-based non-nucleic acid detection

Shanshan Zhang, Dayong Xu, Feng Li and Jin Wang

Trends in Biotechnology, 2025, 43, 2494
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Exonuclease-assisted Cas12a assay without PAM requirement for visual detection of vibrio species

Kenneth Tsz Chun Ng, Derek Han Zhang, Siddharth Raykar and Jiangwen Zhang

Sensors and Actuators B: Chemical, 2025, 426, 137044
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CRISPR/Cas Precisely Regulated DNA-Templated Silver Nanocluster Fluorescence Sensor for Meat Adulteration Detection

Jiahui Chen, Jianghua Liu, Di Wu, Ruiyuan Pan, Jian Chen, Yongning Wu, Mingquan Huang and Guoliang Li

J. Agric. Food Chem., 2022, 70, 14296
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Nucleic acid-assisted CRISPR-Cas systems for advanced biosensing and bioimaging

Siyu Chen, Bo Gong, Cong Zhu, Chunyang Lei and Zhou Nie

TrAC Trends in Analytical Chemistry, 2023, 159, 116931
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Target-independent hybridization chain reaction-fluorescence resonance energy transfer for sensitive assay of ctDNA based on Cas12a

Xuelan Wu, Ting Ju, Zeyang Li, Jingwen Li, Xingwei Zhai and Kun Han

Analytica Chimica Acta, 2023, 1261, 341170
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Recent Advances in the CRISPR/Cas-Based Nucleic Acid Biosensor for Food Analysis: A Review

Yanan Sun, Tianjian Wen, Ping Zhang, Minglian Wang and Yuancong Xu

Foods, 2024, 13, 3222
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Versatile bioluminescence CRISPR/Cas platform for point-of-care detection of biomarkers in whole blood

Xuhua Zhao, Hanxiao Zhang, Yunhua Wang, Minzhi Liao, Siyu Liu, Baofeng Yu, Xinyao Yi, Mengyi Xiong and Xiao-Bing Zhang

Sci. China Chem., 2026, 69, 994
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Proximity-Inducible CRISPR/Cas12a Activity by Scaffold RNA Assembly for Sensing Applications

Yin Gang Nie, Hong-Shuai Zhang, Mei Su, Cheng Jun Zha, Kai Yang and Zhan Ming Ying

Anal. Chem., 2025, 97, 28088
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PAM‐Engineered Toehold Switches as Input‐Responsive Activators of CRISPR‐Cas12a for Sensing Applications

Neda Bagheri, Alejandro Chamorro, Andrea Idili and Alessandro Porchetta

Angewandte Chemie, 2024, 136
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Low-Background CRISPR/Cas12a Sensors for Versatile Live-Cell Biosensing

Qing-Nan Li, Dong-Xia Wang, Gui-Mei Han, Bo Liu, An-Na Tang and De-Ming Kong

Anal. Chem., 2023, 95, 15725
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CRISPR-Cas12a/Cas13a in cancer molecular diagnosis

Juncheng Lin, Yuyuan Wang, Biyun Zeng, Zhibing Chen, Xiaocong Lin and Tao Zeng

Clinica Chimica Acta, 2026, 587, 120934
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Regulating the trans-Cleavage Activity of CRISPR/Cas12a by Using an Elongation-Caged Single-Stranded DNA Activator and the Biosensing Applications

Xinrui Fei, Chao Lei, Wei Ren, Xiaoling Liu and Chenghui Liu

Anal. Chem., 2023, 95, 12169
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CRISPR/Cas12a Protein Switch Powered Label-Free Electrochemical Biosensor for Sensitive Viral Protease Detection

Tianyi Zhang, Yingying Zhao, Cong Zhu, Xi Zhu, Xiaohua Zhu, Ye Qiu, Zhou Nie and Chunyang Lei

Anal. Chem., 2025, 97, 8039
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CRISPR/Cas12a-mediated entropy-driven electrochemical biosensor for detection of genetically modified maize Mon810

Xia Zhu, Jianfeng Zhang, Ronghui Pan, Kai Zhang and Huaxin Dai

Analytica Chimica Acta, 2024, 1296, 342290
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Nanomaterials driven CRISPR/Cas-based biosensing strategies

Ying Huang, Qian Wen, Ying Xiong, Yanni Chen, Wang Li, Jiali Ren and Haiyan Zhong

Chemical Engineering Journal, 2023, 474, 145615
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Integration of electrochemical interface and cell-free synthetic biology for biosensing

Peihang Yu, Chunyang Lei and Zhou Nie

Journal of Electroanalytical Chemistry, 2022, 911, 116209
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PAM‐Engineered Toehold Switches as Input‐Responsive Activators of CRISPR‐Cas12a for Sensing Applications

Neda Bagheri, Alejandro Chamorro, Andrea Idili and Alessandro Porchetta

Angew Chem Int Ed, 2024, 63
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Signal Amplification by the trans-Cleavage Activity of CRISPR-Cas Systems: Kinetics and Performance

Wei Feng, Hongquan Zhang and X. Chris Le

Anal. Chem., 2023, 95, 206
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Engineered CRISPR/Cas Ribonucleoproteins for Enhanced Biosensing and Bioimaging

Linxin Cao, Zeyuan Wang, Chunyang Lei and Zhou Nie

Anal. Chem., 2025, 97, 5866
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RNA-Activated CRISPR/Cas12a Nanorobots Operating in Living Cells

Aijiao Yuan, Rui Sha, Wenjing Xie, Guangbo Qu, Hongquan Zhang, Hailin Wang, X. Chris Le, Guibin Jiang and Hanyong Peng

J. Am. Chem. Soc., 2024, 146, 26657
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Non-canonical CRISPR/Cas12a-based technology: A novel horizon for biosensing in nucleic acid detection

Xueying Lei, Shengnan Cao, Tao Liu, Yongjun Wu and Songcheng Yu

Talanta, 2024, 271, 125663
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Rapid screening of antimicrobial probiotics using CRISPR cascade

Yunhao Lu, Feng Li, Jinrong Bai, Rodrigo Ledesma-Amaro, Dayu Liu, Qiang He and Ruijie Deng

Biosensors and Bioelectronics, 2022, 216, 114673
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CRISPR-HOLMES-based NAD+ detection

Songkuan Zhuang, Tianshuai Hu, Hongzhong Zhou, Shiping He, Jie Li, Yuehui Zhang, Dayong Gu, Yong Xu, Yijian Chen and Jin Wang

Front. Bioeng. Biotechnol., 2024, 12
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