Issue 27, 2020

Enzyme-based optical biosensors for organophosphate class of pesticide detection

Abstract

Organophosphorus pesticides, widely used as insecticides for crop protection, are classified as an extremely toxic class of chemical compounds by the World Health Organization (WHO). Organophosphorus pesticides show marked specificity for the enzyme acetylcholinesterase, and can cause irreversible harm to the nervous system. The excessive use of organophosphorus pesticides, specifically in developing countries, made WHO to impose a ban on certain organophosphorus pesticides being sold to the developing nations. WHO and the Food and Agriculture Organization of United Nations, in joint meetings on pesticide residues, have stressed the need to develop easy, rapid, and sensitive methods to detect the presence of organophosphorus pesticides in food and water. In this regard, a number of review articles have been published explaining a myriad of detection methods for organophosphorus pesticides. Among these, optical detection methods, using biological molecules as the recognition element, provide a number of advantages, such as, high sensitivity and selectivity, simple operation, fast response and cheap instrumentation, and can really prove to be an effective alternative to the time-consuming traditional methods in the current scenario. Therefore, in this review article, we have discussed the recent advances in the field of biosensors employing the optical detection of organophosphorus pesticides. Specifically, various biosensors developed, using enzymes acetylcholinesterase and organophosphate hydrolase, have been categorized on the basis of the material used for their fabrication. A systematic discussion of the working principles, analytical parameters, and advantages and disadvantages of the various biosensors, has been attempted, along with a future perspective, on the challenges and possible improvements in the field of optical detection of organophosphorus pesticides.

Graphical abstract: Enzyme-based optical biosensors for organophosphate class of pesticide detection

Article information

Article type
Perspective
Submitted
27 मार्च 2020
Accepted
10 जून 2020
First published
10 जून 2020

Phys. Chem. Chem. Phys., 2020,22, 15105-15119

Enzyme-based optical biosensors for organophosphate class of pesticide detection

J. Kaur and P. K. Singh, Phys. Chem. Chem. Phys., 2020, 22, 15105 DOI: 10.1039/D0CP01647K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements