Issue 4, 2020

A simple fluorescence aptasensor for gastric cancer exosome detection based on branched rolling circle amplification

Abstract

Exosomes are membrane nanovesicles carrying molecular information that may reflect the biological and genetic characteristics of their parent cells. Numerous studies have demonstrated the potential of exosomes as noninvasive cancer biomarkers. Hence, specific detection of cancer cell-derived exosomes is of significant importance. Here, we developed a fluorescence assay for the determination of gastric cancer exosomes based on branched rolling circle amplification (BRCA) and an aptamer to target specific exosomes. The designed padlock probe was cyclized after incubation with an aptamer binding with the target exosome. BRCA was triggered by adding a second primer and the resulting long tandem double-stranded DNA product was detected using SYBR Green I as the fluorescent dye. This method demonstrated a high specificity for target exosomes with a detection limit of 4.27 × 104 exosomes per mL. Moreover, plasma from gastric cancer patients was tested to verify the clinical applicability of this assay. Our results demonstrated that this aptamer-based biosensor may show potential for the early diagnosis of gastric cancer.

Graphical abstract: A simple fluorescence aptasensor for gastric cancer exosome detection based on branched rolling circle amplification

Article information

Article type
Paper
Submitted
11 अक्तूबर 2019
Accepted
02 दिसम्बर 2019
First published
03 दिसम्बर 2019

Nanoscale, 2020,12, 2445-2451

A simple fluorescence aptasensor for gastric cancer exosome detection based on branched rolling circle amplification

R. Huang, L. He, S. Li, H. Liu, L. Jin, Z. Chen, Y. Zhao, Z. Li, Y. Deng and N. He, Nanoscale, 2020, 12, 2445 DOI: 10.1039/C9NR08747H

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