Volume 207, 2018

Probing the excited state dynamics of Venus: origin of dual-emission in fluorescent proteins

Abstract

Fluorescent proteins exhibit interesting excited state photochemistry, leading to bright fluorescence emission that renders their versatile biological role and wide use as biomarkers. A molecular-level mechanism of the excited state dynamics is desirable to pinpoint the origin of the bright fluorescence of these proteins. Here we present studies on a yellow fluorescent protein variant, Venus, and investigate the photophysics behind the dual fluorescence emission upon UV excitation. Based on our studies, we propose that the unique nature of the potential energy surface is responsible for the observation of minor fluorescence in Venus which is not seen in wild type GFP.

Graphical abstract: Probing the excited state dynamics of Venus: origin of dual-emission in fluorescent proteins

Associated articles

Article information

Article type
Paper
Submitted
04 अक्तूबर 2017
Accepted
06 नवम्बर 2017
First published
06 नवम्बर 2017

Faraday Discuss., 2018,207, 39-54

Probing the excited state dynamics of Venus: origin of dual-emission in fluorescent proteins

S. Dhamija, B. Thakur, P. Guptasarma and A. K. De, Faraday Discuss., 2018, 207, 39 DOI: 10.1039/C7FD00187H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements